Antibodies to the Caveolin Family

Introduction

Caveolins are the principal protein components of caveolae, invaginations of the plasma membrane involved in cell signaling and trafficking.

Caveolins 1, 2 and 3 are a family of integral membrane proteins which are the principal components of caveolae membranes, organizing and concentrating caveolin-interacting proteins and lipids. Caveolins are proteins of about 20 kD. Caveolin-1 spans the plasma membrane twice forming a hairpin structure on the surface and forms homo- and heterooligomers with caveolin-2. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2. Caveolin-3 (Cav-3) or M-caveolin is the muscle-specific isoform of the caveolin family and mutations in the CAV3 gene lead to a large group of neuromuscular disorders.

Caveolins preferentially associate with inactive signalling molecules such as Src and Ras family proteins and have been proposed to act as a scaffold for the assembly of signalling complexes. Caveolin-1 colocalizes and associates with the integrin receptors in vivo. It regulates binding of the Src family kinases to the integrin receptors to promote adh esion and anchorage-dependent growth. Other proposed functions for caveolins include regulation of cell proliferation and tumor suppression.

Antibody Panel to Caveolins

Acris Antibodies offers a range of polyclonal antibodies for the detection of the three currently known caveolins. For caveolin 2 a phospho specific antibody is also available whose immunizing peptide corresponds to amino acid residues 14-25 from mouse phosphocaveolin-2 with tyrosine 19 being phosphorylated.

These antibodies can be used in Western blot, Immunoprecipitation and / or Immunofluorescence.

Fig.1: Western blot of caveolin 1 on rat heart protein extract using SP5142P.

Fig.2: Western blot of caveolin 2 on rat skeletal muscle protein ext ract using SP5143P.

Fig.3: Western blot detection of caveolin 3 from rat cardiac muscle protein extract using SP5144P.

Antibody Panel to Caveolin Family

Caveolin 1-3, Caveolin 2 p-yr19 antibody.

Literature

Traverso M, Gazzerro E, Assereto S, Sotgia F, Biancheri R, Stringara S, Giberti L, Pedemonte M, Wang X, Scapolan S, Pasquini E, Donati MA, Zara F, Lisanti MP, Bruno C, Minetti C. Caveolin-3 T78M and T78K missense mutations lead to different phenotypes in vivo and in vitro. Lab Invest. 2008 Mar;88(3):275-83.

Volonte D, McTiernan CF, Drab M, Kasper M, Galbiati F. Caveolin-1 and caveolin-3 form heterooligomeric complexes in atrial cardiac myocytes that are required for doxorubicin-induced apoptosis. Am J Physiol Heart Circ Physiol. 2008 Jan;294(1):H392-401.

Insel PA, Head BP, Ostrom RS, Patel HH, Swaney JS, Tang CM, Roth DM. Caveolae and lipid rafts: g protein-coupled receptor signalling microdomains in cardiac myocytes. Ann NY Acad Sci. 2005 Jun; 1047: 166-72.

Li XA, Everson WV, Smart EJ. Caveolae, lipid rafts, and vascular disease. Trends Cardiovasc Med. 2005 Apr; 15(3): 92-6.