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Antibody Panel for the Detection of Aurora Kinase Family: Aurora A / STK15 / ARK1, Aurora B / STK12 / ARK2 and Aurora C / STK13 / AIK3

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes.

Zhou et al. (1998) found that STK15 is involved in the induction of centrosome duplication-distribution abnormalities and aneuploidy in mammalian cells. Centrosomes appear to maintain genomic stability through the establishment of bipolar spindles during cell division, ensuring equal segregation of replicated chromosomes to 2 daughter cells. Deregulated duplication and distribution of centrosomes are implicated in chromosome segregation abnormalities, leading to aneuploidy seen in many cancer cell types. In addition amplification of STK15 could be demonstrated in approximately 12% of primary breast tumors, as well as in breast, ovarian, colon, prostate, neuroblastoma, and cervical cancer cell lines. High expression of STK15 mRNA was detected in tumor cell lines without evidence of gene amplification. Ectopic expression of STK15 in mouse NIH 3T3 cells led to the appearance of abnormal centrosome number (amplification) and transformation in vitro. Finally, overexpression of STK15 in near-diploid human breast epithelial cells revealed similar centrosome abnormality, as well as induction of aneuploidy. Aurora kinase A is a key regulatory component of the p53 pathway and overexpression of aurora kinase A leads to increased degradation of p53, causing downregulation of checkpoint-response pathways and facilitating oncogenic transformation of cells. These findings suggested that STK15 is a critical kinase-encoding gene, whose overexpression leads to centrosome amplification, chromosomal instability, and transformation in mammalian cells.

Aurora Kinase B is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

Figure. Figure shows a model of Aurora B, INCENP, and Survivin interactions and regulation. Survivin binds the N-terminus of INCENP, whereas the N terminus of Aurora B binds the C-terminus of INCENP
Figure shows a model of Aurora B, INCENP, and Survivin interactions and regulation. Survivin binds the N-terminus of INCENP, whereas the N terminus of Aurora B binds the C-terminus of INCENP

Human Aurora Kinase C (AURKC, STK13) acts as a chromosomal passenger protein and forms complexes with AURKB and INCENP. AURKC phosphorylated histone H3 in vitro. INCENP bound and activated AURKC in vivo and in vitro, and AURKC coexpressed with INCENP elicited histone H3 phosphorylation of interphase cells. Using an in vitro kinase assay, Yan et al. (2005) found that AURKC-SV could phosphorylate a test substrate protein, and its activity depended on thr179. During cell mitosis, AURKC-SV associated with chromosomes in prophase and metaphase, and then transferred to the central spindle midzone and the cortex where the contracting ring formed during the transition from anaphase to telophase. It remained in the midbody during cytokinesis.

Antibody Panel to Aurora Kinase Subfamily (Aurora A / STK15 antibody, Aurora B / STK12 antibody, Aurora C / STK13 antibody)

Acris Antibodies offers a collection of polyclonal and monoclonal antibodies to members of the Aurora kinase family (Aurora A / STK15 antibody, Aurora B / STK12 antibody, Aurora C / STK13 antibody). These antibodies are usefull for detection of Aurora Kinases. These antibodies are suitable for Western blotting and immunohistochemistry methods, but also other immunological applications, like immunocytochemistry and immunoprecipitation.



Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with a pan-Aurora A monoclonal antibody (clone 35C1), resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against Thr288 phosphorylated Aurora A. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Fig.1: Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with a pan-Aurora A monoclonal antibody (clone 35C1, NB100-1641), resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against Thr288 phosphorylated Aurora A NB600-1157. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Panel B. The blot shown in Panel A was stripped and re-probed with pan-Aurora specific polyclonal antibody to verify equal loading of Aurora A in the extracts. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Fig.2: Panel B. The blot shown in Panel A was stripped and re-probed with pan-Aurora specific polyclonal antibody to verify equal loading of Aurora A in the extracts. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

<b>Detection of Human Phospho-Aurora A (T288) by  Immunocytochemistry.</b> <i>Samples:</i> NBF-fixed asynchronous  HeLa cells. <i>Antibody:</i> Affinity purified rabbit anti-Phospho-  Aurora A (T288) (NB 100-2371) used at a dilution of  1:250. <i>Detection:</I> Anti-rabbit IgG-FITC conjugated (NB 7159) used at a dilution of 1:100.

Fig.3: Detection of Human Phospho-Aurora A (T288) by Immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho- Aurora A (T288) (NB 100-2371) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (NB 7159) used at a dilution of 1:100.

<b>Detection of Human Phospho-Aurora A (T288) by  Immunocytochemistry.</b> <i>Samples:</i> NBF-fixed asynchronous  HeLa cells. Mock phosphatase treated section (left) or calf  intestinal phosphatase-treated section (right) immunostained for  Phospho-Aurora A. <i>Antibody:</i> Affinity purified rabbit anti-  Phospho-Aurora A (T288) (NB 100-2371) used at a  dilution of 1:250. <i>Detection:</i> Anti-rabbit IgG-FITC conjugated  (NB 7159) used at a dilution of 1:100.

Fig.4: Detection of Human Phospho-Aurora A (T288) by Immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-Aurora A. Antibody: Affinity purified rabbit anti- Phospho-Aurora A (T288) (NB 100-2371) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (NB 7159) used at a dilution of 1:100.

Detection of rat Aurora A in mammary gland whole cell tissue lysate (60 ug) using NB 100-635.  6 minute ECL exposure time. *Note: The upper band is believed to be phosphorylated Aurora A.

Fig.5: Detection of rat Aurora A in mammary gland whole cell tissue lysate (60 ug) using NB 100-635. 6 minute ECL exposure time. *Note: The upper band is believed to be phosphorylated Aurora A.

Immunofluorescent localization of mouse Aurora A to centrosomes of a mammary epithelial cell line using NB 100-635.  Detection by Alexa 488 labeled gaot anti-Rabbit IgG (green) and DAPI (blue).

Fig.6: Immunofluorescent localization of mouse Aurora A to centrosomes of a mammary epithelial cell line using NB 100-635. Detection by Alexa 488 labeled gaot anti-Rabbit IgG (green) and DAPI (blue).

<b>Detection of Human Aurora B by Immunohistochemistry.</b> <i>Sample:</i> FFPE section of human colon adenocarcinoma. <i>Antibody:</i> Affinity purified rabbit anti-Aurora B NB 100-1729 used at a dilution of 1:100. <i>Detection:</i> DAB

Fig.7: Detection of Human Aurora B by Immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-Aurora B NB 100-1729 used at a dilution of 1:100. Detection: DAB



Available antibodies to Aurora Kinases

Aurora A antibodies ( STK15 antibody, ARK1 antibody ), Aurora B antibodies ( STK12 antibody, ARK2 antibody ), Aurora C antibodies ( STK13 antibody, AIK3 antibody ) and Aurora A/B antibodies ( STK 12/15 antibody, ARK 1/2 antibody )



 

54 related products - click here to filter product result list

Antibodies


Catalog No. Clone/Isotype Presentation Reactivity Applications  
H00006790-A01 Aurora-A / STK15 / ARK1 antibody  
  - Serum Hu WB 50 µl / ---
Mouse Abnova Taiwan Corp.
 
H00006790-M01 Aurora-A / STK15 / ARK1 antibody  
  5F8 Purified Hu WB 100 µg / ---
Mouse IgG Abnova Taiwan Corp.
 
SM2084B Aurora-A / STK15 / ARK1 antibody  
  35C1 Biotin Hu, Ms IF, WB 0.1 mg / US$ 525
Mouse IgG2b Acris Antibodies GmbH
 
SM2084P Aurora-A / STK15 / ARK1 antibody  
  35C1 Purified Hu, Ms IF, IP, WB 0.1 mg / US$ 495
Mouse IgG2b Acris Antibodies GmbH
 
SP2058P Aurora-A / STK15 / ARK1 antibody  
  - Aff - Purified Hu C, IP 50 µg / US$ 405
Rabbit Acris Antibodies GmbH
 
EB05035 Aurora-A / STK15 / ARK1 antibody  
  - Aff - Purified Hu WB 0.1 mg / ---
Goat Everest Biotech Ltd.
 
GTX12324 Aurora-A / STK15 / ARK1 antibody  
  Aff - Purified Ms, Rt IF, WB 50 µg / ---
Rabbit IgG GeneTex Inc.
 
GTX13408 Aurora-A / STK15 / ARK1 antibody  
  Purified Hu IF, IP, WB 50 µg / ---
Rabbit IgG GeneTex Inc.
 
GTX13824 Aurora-A / STK15 / ARK1 antibody  
  35C1 Ig Fraction Hu, Ms E, IF, IP, WB 100 µl / ---
Mouse IgG2b GeneTex Inc.
 
GTX16596 Aurora-A / STK15 / ARK1 antibody  
  Hu IP 100 µg / ---
Rabbit IgG GeneTex Inc.
 
GTX21287 Aurora-A / STK15 / ARK1 antibody  
  Aff - Purified Hu IP, P 50 µg / ---
Rabbit IgG GeneTex Inc.
 
GTX29689 Aurora-A / STK15 / ARK1 antibody  
  Ig Fraction Hu WB 100 µg / ---
Goat IgG GeneTex Inc.
 
GTX30367 Aurora-A / STK15 / ARK1 antibody  
  Aff - Purified Hu IF, IP, WB 100 µg / ---
Rabbit GeneTex Inc.
 
GTX77628 Aurora-A / STK15 / ARK1 antibody  
  Purified C, IP 50 µg / ---
Rabbit IgG GeneTex Inc.
 
NB100-1641 Aurora-A / STK15 / ARK1 antibody  
  35C1 Purified Hu, Ms E, IF, IP, WB 50 µl / US$ 485
Mouse IgG2b Novus Biologicals Inc.
 
NB100-1829 Aurora-A / STK15 / ARK1 antibody  
  35C1 Biotin Hu, Ms IF, WB 50 µl / US$ 505
Mouse IgG2b Novus Biologicals Inc.
 
NB100-212 Aurora-A / STK15 / ARK1 antibody  
  - Aff - Purified Hu C, IP 0.1 ml / US$ 495
Rabbit Novus Biologicals Inc.
 
NB100-267 Aurora-A / STK15 / ARK1 antibody  
  - Aff - Purified Hu IF, IP, WB 0.1 mg / US$ 485
Rabbit Novus Biologicals Inc.
 
NB100-635 Aurora-A / STK15 / ARK1 antibody  
  - Aff - Purified Ms, Rt IF, WB 0.1 mg / US$ 505
Rabbit Novus Biologicals Inc.
 
NB100-917 Aurora-A / STK15 / ARK1 antibody  
  Aff - Purified Hu, Ms, Rt WB, IP, IF 3 x 0.1 ml / ask
Rabbit Novus Biologicals Inc.
 
NB600-435 Aurora-A / STK15 / ARK1 antibody  
  - Agarose Hu IP 0.4 ml / US$ 485
Rabbit Novus Biologicals Inc.
 
10297-1-AP Aurora-A / STK15 / ARK1 antibody  
  Aff - Purified Hu, Ms E, P, WB 150 µl / ---
Rabbit IgG ProteinTech Group, Inc.
 
NB110-55479 Aurora-A / STK15 / ARK1 (C-term) antibody  
  EP1008Y Purified Hu IF, IP, WB 0.1 ml / US$ 485
Rabbit IgG Novus Biologicals Inc.
 
13040 Aurora-A / STK15 / ARK1 (exon 5) antibody  
  - Aff - Purified Hu C, IP, WB 0.1 mg / ---
Rabbit QED Bioscience Inc.
 
13042 Aurora-A / STK15 / ARK1 (exons 5, 6) antibody  
  - Aff - Purified Hu IF, IP, WB 0.1 mg / ---
Rabbit QED Bioscience Inc.
 
NB100-2371 Aurora-A / STK15 / ARK1 (pThr288) antibody  
  - Purified Hu IF 0.1 ml / US$ 575
Rabbit Novus Biologicals Inc.
 
NB600-1157 Aurora-A / STK15 / ARK1 (pThr288) antibody  
  - Purified Hu WB 0.1 ml / US$ 515
Rabbit Novus Biologicals Inc.
 
SP7343P Aurora-A/B / STK12/15 / ARK1/2 antibody  
  - Purified Hu, Ms, Rt WB 0.1 mg / US$ 405
Rabbit Acris Antibodies GmbH
 
H00009212-M01 Aurora-B / STK12 / ARK2 antibody  
  6A6 Purified Hu WB 100 µg / ---
Mouse IgG Abnova Taiwan Corp.
 
H00009212-M03 Aurora-B / STK12 / ARK2 antibody  
  6H7 Purified Hu WB 100 µg / ---
Mouse IgG Abnova Taiwan Corp.
 
H00009212-M04 Aurora-B / STK12 / ARK2 antibody  
  6G8 Purified Hu WB 100 µg / ---
Mouse IgG Abnova Taiwan Corp.
 
BP4524 Aurora-B / STK12 / ARK2 antibody  
  - Aff - Purified Hu WB 0.2 ml / US$ 515
Sheep Acris Antibodies GmbH
 
R1539P Aurora-B / STK12 / ARK2 antibody  
  - Aff - Purified Hu E, WB 0.1 mg / US$ 465
Rabbit Acris Antibodies GmbH
 
NB100-1729 Aurora-B / STK12 / ARK2 antibody  
  Aff - Purified Hu P 0.1 ml / US$ 565
Rabbit Novus Biologicals Inc.
 
NB100-294 Aurora-B / STK12 / ARK2 antibody  
  - Purified Hu, Ms, Rt, Hst IF, IP, WB 0.1 ml / US$ 435
Rabbit Novus Biologicals Inc.
 
NB500-185 Aurora-B / STK12 / ARK2 antibody  
  - Aff - Purified Hu IF, IP, WB 0.1 mg / ask
Rabbit Novus Biologicals Inc.
 
10792-1-AP Aurora-B / STK12 / ARK2 antibody  
  Aff - Purified Hu, Ms E 150 µl / ---
Rabbit IgG ProteinTech Group, Inc.
 
65031-1-Ig Aurora-B / STK12 / ARK2 antibody  
  Purified Hu E, WB 150 µl / ---
Mouse IgG1 ProteinTech Group, Inc.
 
NB110-55480 Aurora-B / STK12 / ARK2 (N-term) antibody  
  EP1009Y Purified Hu F, IF, IP, WB 0.1 ml / US$ 515
Rabbit IgG Novus Biologicals Inc.
 
A4190-17 Aurora-C / STK13 / AIK3 antibody  
  - Aff - Purified Hu E 40 µg / ---
Rabbit United States Biological
 
NB110-55481 Aurora-C / STK13 / AIK3 (N-Term) antibody  
  EP1011Y Purified Hu WB 0.1 ml / US$ 515
Rabbit IgG Novus Biologicals Inc.
 

Protein


Catalog No. Species Presentation Purity   Source  
H00006790-P01-02 Aurora-A / STK15 / ARK1 antigen  
  Human in vitro transl. 2 µg / ---
      Abnova Taiwan Corp.
 
H00006790-P01-10 Aurora-A / STK15 / ARK1 antigen  
  Human in vitro transl. 10 µg / ---
      Abnova Taiwan Corp.
 
H00006790-P01-25 Aurora-A / STK15 / ARK1 antigen  
  Human in vitro transl. 25 µg / ---
      Abnova Taiwan Corp.
 
H00006790-Q01-10 Aurora-A / STK15 / ARK1 antigen  
  Human in vitro transl. 10 µg / ---
      Abnova Taiwan Corp.
 
H00006790-Q01-25 Aurora-A / STK15 / ARK1 antigen  
  Human in vitro transl. 25 µg / ---
      Abnova Taiwan Corp.
 
H00006790-Q02-10 Aurora-A / STK15 / ARK1 antigen  
  Human in vitro transl. 10 µg / ---
      Abnova Taiwan Corp.
 
H00006790-Q02-25 Aurora-A / STK15 / ARK1 antigen  
  Human in vitro transl. 25 µg / ---
      Abnova Taiwan Corp.
 
H00009212-P01-10 Aurora-B / STK12 / ARK2 antigen  
  Human in vitro transl. 10 µg / ---
      Abnova Taiwan Corp.
 
H00009212-P01-25 Aurora-B / STK12 / ARK2 antigen  
  Human in vitro transl. 25 µg / ---
      Abnova Taiwan Corp.
 
H00009212-Q01-10 Aurora-B / STK12 / ARK2 antigen  
  Human in vitro transl. 10 µg / ---
      Abnova Taiwan Corp.
 
H00009212-Q01-25 Aurora-B / STK12 / ARK2 antigen  
  Human in vitro transl. 25 µg / ---
      Abnova Taiwan Corp.
 
H00006795-Q01-10 Aurora-C / STK13 / AIK3 antigen  
  Human in vitro transl. 10 µg / ---
      Abnova Taiwan Corp.
 
H00006795-Q01-25 Aurora-C / STK13 / AIK3 antigen  
  Human in vitro transl. 25 µg / ---
      Abnova Taiwan Corp.