Antibodies to NPY Receptors (NPYR) - FocusOn 001

Introduction

Neuropeptide Y (NPY) is a neuropeptide colocalized and abundantly released with the neurotransmitter norepinephrine in the nervous system. It is synthesized by the postnatal olfactory epithelium of sustentacular cells. It promotes the proliferation of postnatal neuronal precursor cells and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion and potent vasoactive effects on the cardiovascular system.

Neuropeptide Y family of peptides signal through a family of G protein-coupled receptors present in the brain and sympathetic neurons. These NPY receptors (Y1-Y6) recognize NPY and related peptides.

Antibody Tools for detections of NPY Receptors Type 1 – 5

Acris Antibodies offers an thoroughly validated antibody panel to NPY receptors. These antibodies are useful for the detection of Neuropeptide Y Receptors by Western blotting; most of them can be used in immunohistochemical methods.

NPY1R

Fig. 1: NPY1R Polyclonal Antibody Cat.-No. AP23327PU-N.

Fig. 2: SP4420P NPY1R antibody immunohistochemical staining of Brain.

NPY2R

Fig. 3: NPY Y2 receptor immunoreactivity in Mouse dorsal root ganglion of wild type (A) and NPY Y2 receptor Knockout (B) animals. Note that unspecific staining is observed in thick, possibly myelinated fibers. Image courtesy of Dr. Tomas Hökfelt, Karolinska Institute (Cat.-No. AP20012PU-N).

Fig. 4: AP16644PU-N NPY2R antibody staining of Rat Brain lysate at 0.1 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NPY4R

Fig. 5: Immunohistochemical staining of Brain using anti- PPYR1 antibody SP4422P.

Fig. 6: Immunohistochemical staining of Brain using anti- PPYR1 antibody SP4421P

NPY5R

Fig. 7: Confocal immunofluorescent analysis of NPY5R Antibody (Center) (Cat.-No. AP52926PU-N) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Fig. 8: AP16644PU-N NPY2R antibody staining of Rat Brain lysate at 0.1 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Available antibodies to NPY Receptor

Neuropeptide Y Receptor 1 antibody (NPY1R), Neuropeptide Y Receptor 2 (NPY2R), Neuropeptide Y Receptor 4 (NPY4R) and Neuropeptide Y Receptor 5 (NPY5R)