Antibodies to Progesterone Receptor - FocusOn 049

Introduction

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone.

There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer.

The biological response to progesterone is mediated by two distinct forms of the progesterone receptor (A and B forms). Human PR consists of a 94 kDa A form and a 120 kDa B form. Chicken PR consists of a 78 kDa A form and a 110 kDa B form. In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones. Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor. Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.

Antibody Tools for the detection of Progesterone Receptor

Acris Antibodies offers a panel of antibodies to Progesterone receptor, suitable for immunological detection methods like immunohistochemistry, immunoprecipitation or Western blotting.

Paraffin embedded human breast carcinoma sections stained with DM103 (Fig.1 AEC staining), DM282 (Fig.2 DAB staining) and DRM002 (Fig.3 AEC staining)

Available antibody panel to PR

Progesterone receptor (PR, PGR, NR3C3 = Nuclear receptor subfamily 3 group C member 3) antibody