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Antibodies to Prolactin Receptor - FocusOn 048

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Introduction: Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions.

PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Tools for the Detection of Prolactin Receptor

Acris Antibodies offers a panel of antibodies to PRL receptor, suitable for immunological detection methods like immunohistochemistry, immunofluorescence microscopy or Western blotting. Recombinant Prolactin receptor is also available.



Fig. 1 illustrates immunohistochemical staining of PRL receptor in rat Islets of Langerhans using antibody SM5033P.

Fig. 1 illustrates immunohistochemical staining of PRL receptor in rat Islets of Langerhans using antibody SM5033P.

Fig. 2 illustrates immunohistochemical staining of PRL receptor in NB2 cells using antibody SM5034P.

Fig. 2 illustrates immunohistochemical staining of PRL receptor in NB2 cells using antibody SM5034P.

Available antibody panel to Prolactin Receptor

Prolactin receptor (PRL receptor, PRLR) antibody

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Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
AM00543PU-N

Prolactin receptor antibody

Formalin-fixed, paraffin-embedded human breast cancer stained with Prolactin Receptor (AM00543PU-N) using peroxidase-conjugate and AEC chromogen. Note cell membrane and cytoplasmic staining of tumor cells. Mouse B6.2 Purified Hu P
0.1 mg / €450.00
  IgG1   Acris Antibodies GmbH
 
BP774

Prolactin receptor antibody

Prolactin receptor Sheep Purified Hu, Ms, Rt E, WB
1 ml / €270.00
    Acris Antibodies GmbH
 
SM5033P

Prolactin receptor antibody

Immunohistochemistry with anti-Prolactin Receptor Monoclonal Antibody SM5033P:Mammary gland tissue from lactating light horse mare (equine) was incubated in SM5033P at 1/100 for 1 hour at 23°C and for 24 hours at 4°C. Second antibody was Biotinylated Goat anti-Mouse IgG at 23°C for 1 hour. Development using avidin-biotin complex 23°C for 1 hour plus 3,3-diaminobenzadine for 15 min and counterstained with haematoxylin. Notes: Antigen retrieval was facilitated using steaming at 95°C for 20 min in Trizma® buffer (pH 10.0) . Blocking endogenous peroxidase performed with 0.3% H2O2 and MeOH (20 min at 23°C). To decrease non-specific staining, tissue was pre-incubated in normal goat serum for 30 min at 23°C. Mouse U5 Purified Eq, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €380.00
  IgG1   Acris Antibodies GmbH
+4 additional images
 
SM5034P

Prolactin receptor antibody

Figure 1. Immunofluorescent analysis of Prolactin Receptor in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin Receptor monoclonal antibody SM5034P at a dilution of 1/200 overnight at 4°C, washed with PBS and incubated with DyLight-488 conjugated secondary antibody. Prolactin Receptor staining (Green), F-Actin staining with Phalloidin (Red) and nuclei with DAPI (Blue) is shown. Images were taken at 60X magnification. Mouse T6 Purified Rt C, ICC/IF, P, WB
0.1 mg / €370.00
  IgG2a   Acris Antibodies GmbH
+5 additional images
 
NB100-62862

Prolactin receptor antibody

Prolactin receptor Sheep Purified Hu E, WB
1 ml / €330.00
  IgG   Novus Biologicals Inc.
 
NB300-531

Prolactin receptor antibody

Immunohistochemistry: Prolactin Receptor Antibody (T6) [NB300-531] - Figure 1 shows immunohistochemical staining of PRL receptor in NB2 cells using NB300-531. Mouse T6 Ig Fraction Rt ICC/IF, P, WB
0.1 mg / €370.00
  IgG2a   Novus Biologicals Inc.
 
NB300-561

Prolactin receptor antibody

Immunohistochemistry: Prolactin Receptor Antibody (U5) [NB300-561] - Figure 1 shows immunohistochemical staining of PRL receptor in rat Islets of Langerhans using NB300-561. Mouse U5 Ig Fraction Eq, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB

Regular Price: 0.1 mg / €370.00

Special Price: 0.1 mg / €300.00

  IgG1   Novus Biologicals Inc.
 

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