Fibroblast Marker Antibodies - FocusOn 008
The main function of fibroblasts is to maintain the structural integrity of connective tissue by continuously secreting precursors of the extracellular matrix. Fibroblasts make collagens, glycosaminoglycans, reticular and elastic fibers and glycoproteins found in the extracellular matrix. Many researchers specifically stain fibroblasts to detect cell culture contamination. In the scientific literature several recommendations can be found to achieve this using antibodies to marker proteins specifically expressed by fibroblasts.
Prolyl-4-hydroxylase catalyzes the formation of 4-hydroxyproline in collagens by the hydroxylation of proline residues in peptide linkages. The active enzyme is a tetramer, consisting of two different types of inactive monomers with molecular weights of about 64 kDa (alpha subunit) and 60 kDa (beta subunit).
The monoclonal ER-TR7 antibody detects reticular fibroblasts, which constitute the cellular framework of lymphoid and non-lymphoid organs and their products. ER-TR7 reacts with an intracellular component of fibroblasts.
FSP1/S100A4 is a filament-associated calcium-binding protein and is associated with cells of mesenchymal origin or of fibroblastic phenotype. Polyclonal antisera raised to recombinant fibroblast specific protein 1 (FSP1) stain the cytoplasm of fibroblasts, but not epithelium. FSP1 is also expressed by many tumor cells spreading into distant metastases and is therefore also known as Metastasin.
Acris Antibodies presents a full panel of antibodies useful as fibroblast markers in human, mouse and rat tissues, including ER-TR7 antibody, Prolyl-4-hydroxylase antibodies and FSP1/S100A4 antibodies.
AMC staining of frozen mouse spleen section using rat anti mouse reticular fibroblast antibody ER-TR7 Cat.-No BM4018
Staining of rat spleen paraffin section using mouse anti rat prolyl-4-hydroxylase beta antibody 6-9H6 Cat.-No. AF5110-1
Staining of frozen rat skin section using mouse anti rat prolyl-4-hydroxylase beta (fibroblast marker) antibody 6-9H6 Cat.-No. AF5110-1
Staining of human tonsil paraffin section using mouse anti human prolyl-4-hydroxylase beta (fibroblast marker) antibody 3-2B12 Cat.-No. AF0910-1
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- 2, 4
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