Nesprin antibody - FocusOn 031

Introduction: Nesprins are produced by alternative mRNA splicing and the initiation of two genes, nesprin-1 and nesprin-2 for "nuclear envelope spectrin repeat".

There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed and tissue-specific. Alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus. These proteins are called nesprin-1 and -2. They are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences, and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM-domain-containing sequence essential for perinuclear localization. Nesprin-1 is developmentally regulated in both smooth and skeletal muscle and is re-localized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as \'dystrophins of the nucleus\' to maintain nuclear organization and structural integrity.

Available Antibody for the Detection of Nesprin

Acris Antibodies offers a polyclonal antibody detecting Nesprin-1. The antibody was made using the synthetic polypeptide GRSTPNRQKTPRGKC (residues 3212-3226) conjugated to KLH as immunogen. The antibody can be used at 1:1000 for Western blotting and at 1:50 for immunohistochemistry.

Literature

1. Zhang, Q. et al, Journal of Cell Science (2001) 114, 4485-4498

Available Antibody
Nesprin 1 antibody