Product Description for 293 whole cell lysate
Product is tested for SDS-PAGE, Western blot / Immunoblot.
Properties for 293 whole cell lysate
| Product Category | Lysates |
|---|---|
| Quantity | 0.5 mg |
| Synonyms | Lysates |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Add. information | Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95 degrees C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 0.01-0.02 ml depending on the size format of your gel. |
|---|---|
| Application | Western Blot, Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95 degrees C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 0.01-0.02 ml depending on the size format of your gel. , Western Blot |
| Background | This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins. |
| Concentration | 1 mg/ml |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
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