AR31073PU-N Adiponectin

Search for all "Adiponectin"

25 µg / €220.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Adiponectin

AR31073PU-N

Product Description for Adiponectin

Adiponectin.
Presentation: Purified

Properties for Adiponectin

Product Category Proteins
Target Category
Quantity 25 µg
Synonyms 30 kDa adipocyte complement-related protein, ACDC, ACRP30, ADIPOQ, APM1, Adipocyte, Adipocyte complement-related 30 kDa protein, Adipose most abundant gene transcript 1 protein, C1q and collagen domain-containing protein, GBP28, Gelatin-binding protein
Presentation Purified
Molecular weight 25.8 kDa
Source High-5 Insect cells
Species (Protein) Mouse
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q60994
Purity > 95 % pure by SDS-PAGE and HPLC analyses.
Add. information Centrifuge the vial prior to opening!
Background Adiponectin is an adipose-derived secreted protein containing 236 amino acid residues. It is relatively abundant in humans and rodents, accounting for about 0.01% of total plasma protein. The circulating levels of adiponectin are decreased under conditions of obesity, insulin resistance, and type II diabetes. Disruption of adiponectin in mice causes insulin resistance and neointimal formation. Conversely, administration of recombinant adiponectin suppresses hepatic glucose production, and reverses insulin resistance associated with both lipoatrophy and obesity. The protective role of adiponectin is attributed to its anti-inflammatory properties (e.g. ability to suppress expression of TNF-β and class A scavenger receptor in macrophages).
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Adiponectin is a multimeric glycoprotein containing amino acids Val-21 to Asn-247 of the adiponectin precursor protein fused to an N-terminal histidine tag. Monomeric glycosylated adiponectin migrates at an apparent molecular weight of approximately 35.0 kDa by SDS PAGE analysis under reducing conditions. The calculated molecular weight of Recombinant Murine Adiponectin is 25.8 kDa.
AA Sequence:
RGHHHHHHHH VTTTEELAPA LVPPPKGTCA GWMAGIPGHP GHNGTPGRDG RDGTPGEKGE KGDAGLLGPK GETGDVGMTG AEGPRGFPGT PGRKGEPGEA AYVYRSAFSV GLETRVTVPN VPIRFTKIFY NQQNHYDGST GKFYCNIPGL YYFSYHITVY MKDVKVSLFK KDKAVLFTYD QYQEKNVDQA SGSVLLHLEV GDQVWLQVYG DGDHNGLYAD NVNDSTFTGF LLYHDTN
Biological activity:
Determined by a cytotoxic assay using M1 cells.
The ED50 for this effect is 4.0-6.0 μg/ml.
Format
Buffer System:
20mM Tris, pH 8.5 + 75mM L-Arginine
Endotoxin level:
< 0.1 ng per µg (< 1EU/µg)
Purity:
>95% pure by SDS-PAGE and HPLC analyses.
State:
Lyophilized (sterile filtered) protein
Purified
Reconstitution:
Restore in water to a concentration of 0.1-1.0 mg/ml. 
Note: Allow the reconstituted vial to sit at room temperature for 30 minutes before use. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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