Product Description for AKR1B1 / ALDR1
AKR1B1 / ALDR1.
Properties for AKR1B1 / ALDR1
|Molecular weight||35.8 kDa|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Purity||> 95 %|
|Background||Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.|
|Concentration||1.0 mg/ml (determined by Bradford assay)|
1. Prepare a 1 ml reaction mix into a suitable container :
The final concentrations are 0.1M sodium phosphate (pH7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH.
2. Add 50ul of recombinant AKR1B1 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer.
3. Mix by inversion and incubate at 25°C for 2.5 minutes.
4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.
5. Record the increase in A340nm for 3 minutes.
|General Readings||Ruiz FX., et al: (2009) Chem Biol Interact.178(1-3).
Donaqhue KC., et al: Diabet Med. 22(10):1315-20.
|Storage||Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.