Product Description for AKR1B1 / ALDR1
AKR1B1 / ALDR1.
Presentation: Purified
Presentation: Purified
Properties for AKR1B1 / ALDR1
| Product Category | Proteins |
|---|---|
| Quantity | 0.5 mg |
| Synonyms | Aldose reductase |
| Presentation | Purified |
| Molecular weight | 35.8 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 95 % |
| Background | Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay 1. Prepare a 1 ml reaction mix into a suitable container : The final concentrations are 0.1M sodium phosphate (pH7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR1B1 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and incubate at 25°C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase in A340nm for 3 minutes. |
| General Readings | Ruiz FX., et al: (2009) Chem Biol Interact.178(1-3). Donaqhue KC., et al: Diabet Med. 22(10):1315-20. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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