GST-Tag (1-224) - AR03011PU-N | acris-antibodies.com

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Product Description for GST-Tag

GST-Tag.
Presentation: Purified

Properties for GST-Tag

Product Category	Proteins
Quantity	0.1 mg
Synonyms	GST26-Tag, Glutathione S-transferase Tag
Presentation	Purified
Molecular weight	26 kDa
Source	E. coli
Species (Protein)	Schistosoma
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P08515
Purity	> 95 %
Background	Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E.coli source.
Concentration	1.0 mg/ml
General Readings	Hayes JD., et al. (1995). Crit Rev Biochem Mol Biol. 30(6):445-600. Bekett GJ., et al. (1987). J. Clin Biochem Nutr. 2, 1-24. Smith DB., et al. (1988). Mol Biochem Parasitol. 27(2-3):249-56.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	AA Sequence: `MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR` Biological activity: 0.5-2.5 units/mg (please enquire for specific batch value). A unit is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C. Activity Assay 1.Prepare a 1 ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2.Equilibrate to 25°C and monitor at A340nm until the value is constant using a spectrophotometer. 3.Add 50 ul of GST protein with various concentrations (1ug, 2ug, 5ug) in 950 ul reaction buffer. 4.Mix by inversion and record the increase at A340nm for 5 minutes. Molecular weight: (224 aa)
Format	Buffer System: Phosphate Buffered Saline pH 7.4 Endotoxin level: < 1.0 EU per 1 ug of protein (determined by LAL method) Purity: by SDS PAGE State: Liquid purified protein Purified
Targets	GST-Tag antibody

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