PA040 Interleukin-6 (IL6)

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2 µg / €130.00
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Interleukin-6 (IL6)

PA040

Product Description for Interleukin-6 (IL6)

Interleukin-6 (IL6).

Properties for Interleukin-6 (IL6)

Product Category Proteins
Quantity 2 µg
Synonyms B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFNB2, IL-6, Interferon beta-2
Molecular weight 21.7 kDa
Source E. coli
Species (Protein) Rat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P20607
Purity > 95 %
Add. information Centrifuge the vial prior to opening!
Background IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R alpha and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells.
General Readings
  • Yu XW, Chen Q, Kennedy RH, Liu SJ. Inhibition of sarcoplasmic reticular function by chronic interleukin-6 exposure via iNOS in adult ventricular myocytes. J Physiol. 2005 Jul 15;566(Pt 2):327-40. Epub 2005 Apr 21. PubMed PMID: 15845578.
  • Yu XW, Liu MY, Kennedy RH, Liu SJ. Both cGMP and peroxynitrite mediate chronic interleukin-6-induced negative inotropy in adult rat ventricular myocytes. J Physiol. 2005 Jul 15;566(Pt 2):341-53. Epub 2005 May 5. PubMed PMID: 15878941.
  • Bruce CR, Dyck DJ. Cytokine regulation of skeletal muscle fatty acid metabolism: effect of interleukin-6 and tumor necrosis factor-alpha. Am J Physiol Endocrinol Metab. 2004 Oct;287(4):E616-21. Epub 2004 May 18. PubMed PMID: 15149950.
  • Rühl A, Franzke S, Collins SM, Stremmel W. Interleukin-6 expression and regulation in rat enteric glial cells. Am J Physiol Gastrointest Liver Physiol. 2001 Jun;280(6):G1163-71. PubMed PMID: 11352809.
  • Iwai M, Cui TX, Kitamura H, Saito M, Shimazu T. Increased secretion of tumour necrosis factor and interleukin 6 from isolated, perfused liver of rats after partial hepatectomy. Cytokine. 2001 Jan 7;13(1):60-64. PubMed PMID: 11145844.
Storage Store the lyophilized protein at -20°C.
Reconstituted rat IL-6 should be stored in working aliquots
with a carrier protein at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Source E.coli
Description
AA Sequence:
MFPTSQVRRG DFTEDTTHNR PVYTTSQVGG LITYVLREIF EMRKELCNGN SDCMNSDDAL SENNLKLPEI QRNDGCFQTG YNQEICLLKI CSGLLEFRFY LEFVKNNLQD NKKDKARVIQ SNTETLVHIF KQEIKDSYKI VLPTPTSNAL LMEKLESQKE WLRTKTIQLI LKALEEFLKV TMRSTRQT
Biological activity:
The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is < 0.01 ng/ml.
Specific activity:
> 1 x 10e8 units/mg
Format
Buffer System:
10 mM Tris, pH 8.0 + 0.1% SDS
Endotoxin level:
< 0.1 ng per µg (1EU/µg)
Purity:
> 98% pure by SDS-PAGE and HPLC analyses.
State:
Lyophilized (sterile filtered) purified fraction.
Reconstitution:
Restore in water to a concentration of 0.1-1.0 mg/ml.
This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or
-20°C for future use.
Targets