Product Description for Superoxide Dismutase 1 / SOD1
Properties for Superoxide Dismutase 1 / SOD1
|Synonyms||ALS1, CuZn-SOD, CuZnSOD, IPOA, SOD-1, Superoxide dismutase [Cu-Zn]|
|Molecular weight||15.9 kDa|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Proteins (de)|
|Purity||> 95 % pure by SDS-PAGE.|
|Background||Superoxide dismutase 1 (SOD1) binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. The encoded protein neutralizes supercharged oxygen molecules, which can damage cells if their levels are not controlled. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS).|
|Concentration||1.0 mg/ml (determined by Bradford assay)|
1.Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50 mM potassium phosphate, 0.1 mM ethylendiaminetetraacetic acid, 0.01 mM cytochrom C, 0.05 mM xanthine, 0.005 units xanthine oxidase.
2.Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3.Add 50 ul of recombinant SOD protein in various concentrations (0.5ug, 1ug) in assay buffer.
4.Mix by inversion and record the increase at A550nm for 5 minutes.
|Storage||Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
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