AR03033PU-L Superoxide Dismutase 1 / SOD1 (1-154)

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0.5 mg / €750.00
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Superoxide Dismutase 1 / SOD1

Product Description for Superoxide Dismutase 1 / SOD1

Superoxide Dismutase 1 / SOD1.
Presentation: Purified

Properties for Superoxide Dismutase 1 / SOD1

Product Category Proteins, Peptides & Growth Factors
Target Category
Quantity 0.5 mg
Synonyms ALS1, CuZn-SOD, CuZnSOD, IPOA, SOD-1, Superoxide dismutase [Cu-Zn]
Presentation Purified
Molecular weight 15.9 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P00441
Purity > 95 % pure by SDS-PAGE.
Background Superoxide dismutase 1 (SOD1) binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. The encoded protein neutralizes supercharged oxygen molecules, which can damage cells if their levels are not controlled. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS).
Concentration 1.0 mg/ml (determined by Bradford assay)
Protocols Activity Assay
1.Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50 mM potassium phosphate, 0.1 mM ethylendiaminetetraacetic acid, 0.01 mM cytochrom C, 0.05 mM xanthine, 0.005 units xanthine oxidase.
2.Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3.Add 50 ul of recombinant SOD protein in various concentrations (0.5ug, 1ug) in assay buffer.
4.Mix by inversion and record the increase at A550nm for 5 minutes.
General Readings
  1. Conwit R ., et al.,(2006) Journal of the Neurological Sciences 251 (1-2).
  2. Banci L., et al.,(2008) PLoS ONE. Feb 27;3(2):e1677.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant SOD1 was expressed in E.coli and purified by conventional chromatography techniques.
AA Sequence:
MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ
Biological activity:
Specific activity is > 90 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25°C in a 1.5 ml reaction volume (see "Protocols").
Molecular weight:
(154 aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris pH 7.5, 10% Glycerol
Purity:
>95% pure by SDS-PAGE.
State:
Liquid purified protein.
Purified
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