DA3535S VEGFR-3 / Flt-4 (His-tagged)

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5 µg / €160.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

VEGFR-3 / Flt-4

DA3535S

Product Description for VEGFR-3 / Flt-4

VEGFR-3 / Flt-4.
Presentation: Purified

Properties for VEGFR-3 / Flt-4

Product Category Proteins
Target Category
Quantity 5 µg
Synonyms FLT4, Tyrosine-protein kinase receptor FLT4, VEGF Receptor 3, VEGFR3, Vascular endothelial growth factor receptor 3
Presentation Purified
Molecular weight 120 kDa
Source Insect cells
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P35916
Purity > 90 % by SDS-PAGE and visualised by silver stain
Add. information Centrifuge vials before opening!
Background Recombinant human soluble Vascular Endothelial Growth Factor Receptor-3 (sVEGFR-3/FLT-4) was fused with a carboxy-terminal 6X histidine-tag. All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin-like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The FLT-4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751 aa residue extracellular domain, a 22 aa transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4. The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stages of development. It is important for lymphangiogenesis.
General Readings
  1. Joukov et al., EMBO J 15:290, 1996.
  2. Kukk et al., Development 122:3829, 1996.
Storage Prior to and following reconstitution store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
The recombinant mature sVEGFR-3/FLT-4 is a glycosylated monomeric protein with a mass of approximately 120 kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Glu774).
Biological activity:
Measured by its ability to bind recombinant rat VEGF-C in a functional solid phase binding assay.
Immobilised recombinant human sVEGFR-3/FLT-4 at 5 µg/ml can bind recombinant rat VEGF-C in a linear range of 8-500 ng/ml.
Format
Endotoxin level:
< 0.1 ng per µg of sVEGFR-3
Purity:
>90% by SDS-PAGE and visualised by silver stain
State:
Lyophilized without buffer and stabilizer.
Purified
Reconstitution:
Restore in PBS or medium to a concentration not lower than 100 µg/ml.
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