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Product Description for Apolipoprotein E (Apo E)
Properties for Apolipoprotein E (Apo E)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 0.5 mg |
| Reactivity | Human |
| Presentation | Purified |
| Applications | Frozen sections, Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot, Immunoprecipitation |
| Host | Goat |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | ApoE, Apo-E, FocusOn036 |
| Swiss Prot Num | P02649 |
| Immunogen | Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification. |
| Product Type | Antibodies |
| Storage | Store the antibody (undiluted) at 2-8°C for up to one month. For extended storage, mix with an equal volume of glycerol, aliquots contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. Shelf life one year from despatch. |
| Format | State: Liquid (sterile filtered) purified Ig fraction |
| Purification: Immunoaffinity chromatography | |
| Buffer System: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0, containing 0.01% Sodium Azide as preservtive | |
| Specificity | This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other Apolipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human Apolipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J Apolipoproteins. Specific cross reaction of anti-Apolipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-Apolipoprotein antibodies with other human serum proteins is negligible. |
| Application | Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Recommended dilutions: ELISA: 1/4,000-1/8,000 Western blot: 1/500-1/2,000 Immunohistochemistry: 1/50-1/200 |
| Background | Apolipoprotein E is essential for the normal catabolism of triglyceride rich lipoprotein constituents. The apolipoprotein E gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in Apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Mutations in the APOE gene confer susceptibility to Alzheimer's disease by affecting amyloid-beta deposition. |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Focuson | 036 Antibodies to Apolipoprotein |
Secondary Antibodies for Apolipoprotein E (Apo E)
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1317AP | Goat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 180 Euro | |||||
| R1317B | Goat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170 Euro | |||||
| R1317F | Goat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160 Euro | |||||
| R1317HRP | Goat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 170 Euro | |||||
| R1317T | Goat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160 Euro | |||||
| R1416R | Goat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1435C2 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy2 | 1 mg / 250 Euro | |||||
| R1435C3 | Goat IgG (H&L) | ||||||
| Donkey | Cy3 | 1 mg / 250 Euro | |||||
| R1435C5 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy5 | 1 mg / 250 Euro | |||||
