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Product Description for Mouse IgG (H&L)
Properties for Mouse IgG (H&L)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 2 mg |
| Presentation | Biotin |
| Host | Rabbit |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Immunogen | Mouse IgG whole molecule. |
| Product Type | Secondary antibodies |
| Storage | Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation) |
| Format | Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| State: Lyohilized purified Ig fraction. | |
| Purity: and | |
| Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) | |
| Molar radio: 10-20 BAC molecules per Rabbit IgG molecule | |
| Purification: Immunoaffinity Chromatography. | |
| Buffer System: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium azide as preservative. | |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. |
| Application | Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/300,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |