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Product Description for Mouse IgG (H&L)
Properties for Mouse IgG (H&L)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 2 mg |
| Presentation | FITC |
| Applications | Flow cytometry, Immunofluorescence |
| Host | Rabbit |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Immunogen | Mouse IgG whole molecule. |
| Product Type | Secondary antibodies |
| Storage | Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2-8° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. |
| References | 1. The and Feltkamp, Immunology 18; 865, 1970. (Conjugation) |
| Format | Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| State: Lyophilized purified IgG fraction. | |
| Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) | |
| Absorption emission: 495 nm / 528 nm | |
| Molar radio: 3.7 moles FITC per mole of Rabbit IgG | |
| Purification: Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. | |
| Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. | |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum. |
| Application | Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000. |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |