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Product Description for Mouse IgG (H&L)
Properties for Mouse IgG (H&L)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 2 mg |
| Presentation | HRP |
| Host | Rabbit |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Immunogen | Mouse IgG whole molecule. |
| Product Type | Secondary antibodies |
| Storage | Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | 1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation) |
| Format | Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| State: Lyophilized purified Ig fraction. | |
| Purity: and | |
| Label: Horseradish Peroxidase (HRP) | |
| Purification: Immunoaffinity Chromatography. | |
| Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer. | |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. |
| Application | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions ELISA: 1/10,000-1/100,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
| Caution | Do Not Add Sodium Azide. |
| Concentration | 2.0 mg/ml (by UV absorbance at 280nm) |
