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Product Description for Mouse IgG (H&L)
Properties for Mouse IgG (H&L)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 2 mg |
| Presentation | Texas Red |
| Host | Rabbit |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Immunogen | Mouse IgG whole molecules. |
| Product Type | Secondary antibodies |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol Methods 50; 193, 1982. (Conjugation) |
| Format | Reconstitution: Restore with 1.0 ml of deionized water(or equivalent). |
| State: Lyophilized purified Ig fraction. | |
| Purity: and | |
| Label: Texas Red(TM) Sulfonyl Chloride (Molecular Weight 625 daltons). | |
| Absorption emission: 596 nm / 620 nm | |
| Molar radio: 3.3 moles Texas Red(TM) per mole of Rabbit IgG) | |
| Purification: Immunoaffinity chromatography. | |
| Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide as preservative. | |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. |
| Application | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring high titer binding and lot-to-lot consistency. Recommended dilutions: Flow cytometry: 1:2,000-1:10,000. IF microscopy: 1:500-1:2,500. |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |