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Product Description for Goat IgG (H&L)
Properties for Goat IgG (H&L)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 2 mg |
| Presentation | Biotin |
| Host | Rabbit |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Immunogen | Goat IgG, whole molecule. |
| Product Type | Secondary antibodies |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation) |
| Format | Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| State: Lyophilized purified Ig fraction. | |
| Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). | |
| Molar radio: 10-20 BAC molecules per Rabbit IgG molecule. | |
| Purification: Immunoaffinity Chromatography. | |
| Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. | |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG and Goat Serum. |
| Application | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other assays using streptavidin/avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/1,000,000. Western Blot: 1/20,000-1/100,000. Immunohistochemistry: 1/10,000-1/50,000. |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
