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Product Description for Mouse IgA + IgG + IgM (H&L)
Properties for Mouse IgA + IgG + IgM (H&L)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 2 mg |
| Presentation | FITC |
| Host | Goat |
| Shipping to | Worldwide |
Datasheet Extract
| Immunogen | Mouse IgG, IgA and IgM wohle molecules. |
| Product Type | Secondary antibodies |
| Storage | Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. Shelf life: One year from despatch. |
| References | The and Feltkamp, Immunology 18; 865, 1970. (Conjugation) |
| Format | Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| State: Lyophilized purified Ig fraction. | |
| Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons). | |
| Absorption emission: 495 nm / 528 nm | |
| Molar radio: 3.4 moles FITC per mole of Goat IgG. | |
| Purification: Immunoaffinity Chromatography. | |
| Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. | |
| Specificity | This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM. This reagent is suitable for the detection of all mouse isotypes and chain combinations. |
| Application | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended dilutions: FACS: 1/2,000-1/10,000. IF microscopy: 1/500-1/2,500. |
| Concentration | 2.0 mg/ml (by UV absorbance at 280nm) |
