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Product Description for Mouse IgA + IgG + IgM (H&L)
Properties for Mouse IgA + IgG + IgM (H&L)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 2 mg |
| Presentation | HRP |
| Host | Goat |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Immunogen | Mouse IgG, IgA and IgM whole molecules. |
| Product Type | Secondary antibodies |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Nakane, J. Immunol. Methods 47; 129-144. 1981 (Conjugation). |
| Format | Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| State: Lyophilized purified Ig fraction. | |
| Label: Horseradish Peroxidase (HRP). | |
| Purification: Immunoaffinity Chromatography. | |
| Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Thimerosal as preservative. | |
| Specificity | This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. This reagent is suitable for the detection of all mouse immunoglobulin isotypes and chain combinations. |
| Application | Suitable for Immunoblotting (1/500-1/3,000 for Western blot), ELISA (1/100,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/250-1/1,500) as well as other Peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. |
| Caution | Do Not Use Sodium Azide as preservative. |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
