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R1364AP Rabbit IgG (H&L) antibody


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1 mg / €190.00
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Quick Overview

Goat anti Rabbit IgG (H&L)

Product Description for Rabbit IgG (H&L)

Properties for Rabbit IgG (H&L)

ManufacturerAcris Antibodies GmbH
Quantity1 mg
PresentationAP
HostGoat
PDF datasheetview
Shipping toWorldwide

Datasheet Extract

SynonymsRabbit Immunoglobulin G
Immunogen Rabbit IgG, whole molecule.
Product TypeSecondary antibodies
StorageStore the antibody undiluted at 2-8°C. DO NOT FREEZE! Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Shelf life: one year from despatch.
References1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175, 1971. (Conjugation)
FormatState: Liquid (sterile filtered) purified Ig fraction.
 Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
 Purification: Immunoaffinity Chromatography.
 Buffer System: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0 containing 0.09% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
Specificity This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG and Rabbit Serum.
ApplicationSuitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/3,000-1/10,000. Western blot: 1/1,000-1/4,000. Immunohistochemistry: 1/200-1/1,000.
Concentration1.0 mg/ml (by UV absorbance at 280 nm).