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Product Description for Rabbit IgG (H&L)
Properties for Rabbit IgG (H&L)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 2 mg |
| Presentation | FITC |
| Applications | Flow cytometry, Immunofluorescence |
| Host | Goat |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | Rabbit Immunoglobulin G |
| Immunogen | Rabbit IgG, whole molecule. |
| Product Type | Secondary antibodies |
| Storage | Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent; this product is stable for one month at 2-8°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after restoration and standing at room temperature. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Protect from light. Shelf life: One year from despatch. |
| References | 1. The and Feltkamp, Immunology 18; 865, 1970. (Conjugation) |
| Format | Reconstitution: Restore with 1.0 ml of deionized water or equivalent. |
| State: Lyophilized purified Ig fraction. | |
| Label: Fluorescein isothiocyanate (FITC) Molecular Weight 390 daltons | |
| Absorption emission: 495 nm / 528 nm | |
| Molar radio: 3.7 moles FITC per mole of Goat IgG | |
| Purification: Immunoaffinity Chromatography. | |
| Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer | |
| Specificity | This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum. |
| Application | Suitable for Immunofluorescence Microscopy (1/500-1/2,500) and Flow Cytometry (1/2,000-1/10,000) as well as other antibody based fluorescent assays like FLISA or fluorescent Western blotting. |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |