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R1364HRP Rabbit IgG (H&L) antibody

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2 mg / €170.00
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Delivery time: 24 hours
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Quick Overview

Goat anti Rabbit IgG (H&L)

Product Description for Rabbit IgG (H&L)

Properties for Rabbit IgG (H&L)

ManufacturerAcris Antibodies GmbH
Quantity2 mg
PresentationHRP
HostGoat
Shipping toWorldwide

Datasheet Extract

SynonymsRabbit Immunoglobulin G
Immunogen Rabbit IgG, whole molecule.
Product TypeSecondary antibodies
StorageStore vial at 2-8°C prior to restoration.
Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
References1. Farr & Nakane, J. Immunol. Methods 47; 129-144, 1981. (Conjugation)
FormatReconstitution: Restore with 1.0 ml of deionized water (or equivalent).
 State: Lyophilized purified Ig fraction.
 Label: Horseradish Peroxidase (HRP)
 Purification: Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads.
 Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stablizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
Specificity Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
ApplicationSuitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/270,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/5,000.
CautionDo Not Add Sodium Azide.
Concentration2.0 mg/ml (by UV absorbance at 280 nm)