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Product Description for Rabbit IgG (H&L)
Properties for Rabbit IgG (H&L)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 1 mg |
| Presentation | Cy3 |
| Host | Goat |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | Rabbit Immunoglobulin G |
| Immunogen | Rabbit IgG, whole molecule. |
| Product Type | Secondary antibodies |
| Storage | Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after restoration and standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | Conjugation Reference: Modified from GE Healthcare Bio-Sciences Corp., Piscataway, NJ. |
| Format | Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| State: Lyophilized purified IgG fraction | |
| Label: Cy3 (Molecular Weight 949 daltons) <b>Conjugation Chemistry:</b> N-hydoxysuccinimide (NHS) ester | |
| Absorption emission: 552 nm / 565 nm | |
| Molar radio: 10.1 moles Cy3 per mole of Goat IgG (acceptable range: 6-20) | |
| Purification: Immunoaffinity Chromatography. | |
| Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. | |
| Specificity | This product was prepared from monospecific antiserum by Assay by immuno-electrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins. |
| Application | This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000 Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000. |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
