AP22130PU-S RAD17 pSer645 antibody

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20 µg / €330.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Bovine, Equine, Human, Monkey, Mouse RAD17

AP22130PU-S

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  • AP22130PU-S

Product Description for RAD17

Rabbit anti Bovine, Equine, Human, Monkey, Mouse RAD17.
Properties: pSer645
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for RAD17

Product Category Primary Antibodies
Target Category
Quantity 20 µg
Synonyms Cell cycle checkpoint protein RAD17, R24L, RF-C/activator 1 homolog
Presentation Aff - Purified
Reactivity Bov, Eq, Hu, Mky, Ms
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O75943
Immunogen:
Human Rad17 protein containing a phosphorylated serine residue at position 645
Genename:
5884
Remarks:
The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, CRA_f and CRA_d), rhesus monkey, chimpanzee and orangutan, 92% homologous in horse, cow and mouse and 85% homologous in dog and rat.
Property pSer645
Application Immunohistochemistry on paraffin sections: 5 µg/ml.
Western blot: 0.025-1 µg/ml.
Background Rad17, also known as 'cell cycle checkpoint protein RAD17,' is a nuclear protein belonging to the Rad17/RAD24 family and necessary for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Rad17 is known to have a weak ATPase activity and is required for binding to chromatin and also participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. It may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination. Rad17 is a part of a DNA-binding complex containing RFC2, RFC3, RFC4 and RFC5. Four isoforms have been reported by X-ray irradiation. Overexpression of this protein is seen in various cancer cell lines and in colon carcinoma.
Concentration 0.2 mg/ml
General Readings
  1. Bao, S. et al. Cancer Res. 59:2023-2028 (1999).
  2. Parker, AE. et al. J. Biol. Chem. 273:18340-18346 (1998). Note: Erratum: J. Biol. Chem. 274:24438-24439 (1999).
  3. Bao, S. et al. Nature. 411:969-974 (2001).
Storage Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Antigen affinity chromatography
Buffer System:
PBS containing 0.05% BSA and 0.05% sodium azide
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Cow, Dog, Horse, Rat
Species reactivity (tested):
Chimpanzee, Human, Mouse, Orangutan, Rhesus Monkey
Specificity
Specificity:
This antibody detects RAD17 pSer645.
Gene ID 5884

Accessory Products

Proteins and/or Positive Controls

Proteins for RAD17 (3 products)

Catalog No. Species Pres. Purity   Source  
H00005884-P01

RAD17

RAD17 Human Purified
2 µg / €250.00
  Abnova Taiwan Corp.
H00005884-Q01-10

RAD17

RAD17 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00005884-Q01-25

RAD17

RAD17 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for RAD17 (3 products)

Catalog No. Species Pres. Purity   Source  
H00005884-T01

RAD17 293T Cell Transient Overexpression Lysate(Denatured)

RAD17 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.
NBL1-15111

Rad17 Lysate

Western Blot: Rad17 Lysate [NBL1-15111] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for RAD17
0.1 mg / €370.00
  Novus Biologicals Inc.
NBL1-15112

Rad17 Lysate

Western Blot: Rad17 Lysate [NBL1-15112] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for RAD17
0.1 mg / €370.00
  Novus Biologicals Inc.
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