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R1370HRP Rat IgG (H+L chain)

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1 mg / €200.00
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Rat IgG

Product Description for Rat IgG

Properties: (H+L chain)
Presentation: HRP

Properties for Rat IgG

Product Category Secondary Antibodies
Quantity 1 mg
Presentation HRP
Host Chicken
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Immunogen:
Rat IgG whole molecules.
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:12,000 of the reconstitution concentration is suggested for this product.
Concentration 1.0 mg/ml
References 1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA IgG and Protease free) and 0.01% (w/v) Gentamicin Sulfate as preservative.
Purity:
and
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Rat IgG and Rat Serum.