Antibodies to ATPase Enzymes - Product Review 12

Introduction

ATPase proteins are enzymes which are able to supply energy by cleaving ATP into ADP and phosphate. This energy can be used for transport of ions into or out of the cell against a concentration gradient. There are several forms of membrane bound ATPase enzymes responsible for export or import of different ions.

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.

The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium ATPases (SERCA). The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha- subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.

Antibody Tools for Detection of Membrane Bound ATPase Enzymes

Acris Antibodies offers a wide range of antibodies specific for the different types of membrane bound ATPase enzymes and their respective isoforms. Monoclonal and polyclonal antibodies useful for multiple applications are listed in the table below. Species reactivity is broad in most cases due to high conservation of these proteins. Pricing and product informations are available using the links provided.

Available products

ATPase, PMCA, SERCA antibody

18 Item(s)

per page
18 products - FILTER YOUR RESULTS!

Antibodies

Catalog No. Host Clone/Iso. Pres. React. Applications  
SM5109P

ATP1A3 antibody

ATP1A3 Mouse XVIF9-G10 Purified Can, GP, Hu, Prim, Rb, Rt, Sh C, F, ICC/IF, WB
0.1 mg / €380.00
  IgG1   Acris Antibodies GmbH
 
H00000482-M04

ATP1B2 antibody

Detection limit for recombinant GST tagged ATP1B2 is approximately 3ng/ml as a capture antibody. Mouse 4E3 Purified Hu E, WB
0.1 mg / €340.00
  IgG2a   Abnova Taiwan Corp.
 
H00000489-M02

ATP2A3 / SERCA3 antibody

ATP2A3 / SERCA3 Mouse 1B9 Purified Hu E, WB
0.1 mg / €340.00
  IgG2a   Abnova Taiwan Corp.
 
SM5057P

ATP2B4 / PMCA4 antibody

Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/100 with SM5057P or without primary antibody (negative control) overnight at 4°C in a humidifier chamber. Mouse JA9 Purified Hu, Por, Rt C, E, F, ICC/IF, P, WB
0.1 mg / €380.00
  IgG1   Acris Antibodies GmbH
 
H00027032-B01P

ATP2C1 antibody

Western Blot analysis of ATP2C1 expression in transfected 293T cell line (H00027032-T01) by ATP2C1 MaxPab polyclonal antibody.

Lane 1: ATP2C1 transfected lysate(101.09 KDa).
Lane 2: Non-transfected lysate.
Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €340.00
    Abnova Taiwan Corp.
 
H00027032-D01P

ATP2C1 antibody

ATP2C1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ATP2C1 expression in HepG2. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €360.00
    Abnova Taiwan Corp.
 
H00000496-B01P

ATP4B antibody

Western Blot analysis of ATP4B expression in transfected 293T cell line (H00000496-T01) by ATP4B MaxPab polyclonal antibody.

Lane 1: ATP4B transfected lysate(32.12 KDa).
Lane 2: Non-transfected lysate.
Mouse Purified Hu E(detection), WB
50 µg / €340.00
    Abnova Taiwan Corp.
 
H00000496-D01P

ATP4B antibody

Western Blot analysis of ATP4B expression in transfected 293T cell line (H00000496-T03) by ATP4B MaxPab polyclonal antibody.

Lane 1: ATP4B transfected lysate(32.12 KDa).
Lane 2: Non-transfected lysate.
Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €360.00
    Abnova Taiwan Corp.
 
H00000496-M10

ATP4B antibody

Detection limit for recombinant GST tagged ATP4B is approximately 0.1ng/ml as a capture antibody. Mouse 1D10 Purified Hu E, WB
0.1 mg / €340.00
  IgG2a   Abnova Taiwan Corp.
 
GTX72399

Calcium Pump pan PMCA ATPase antibody

Calcium Pump pan PMCA ATPase Mouse 5F10 Purified Hu WB
0.5 ml / €330.00
  IgG1   GeneTex Inc.
 
H00000483-B01P

CD298 / ATP1B3 antibody

ATP1B3 MaxPab polyclonal antibody. Western Blot analysis of ATP1B3 expression in A-431. Mouse Purified Hu E(detection), F, WB
50 µg / €340.00
    Abnova Taiwan Corp.
 
H00000483-D01P

CD298 / ATP1B3 antibody

ATP1B3 MaxPab rabbit polyclonal antibody. Western Blot analysis of ATP1B3 expression in PC-12. Rabbit Purified Hu, Rt E(detection), WB
0.1 mg / €360.00
    Abnova Taiwan Corp.
 
GTX30202

TBK1 antibody

TBK1 Mouse 464.6, alpha-1 subunit, also known as clone 6H Purified Can, Hu, Mky, Rb, Rt, Sh, Xen C, WB
50 µl / €390.00
    GeneTex Inc.
 
GTX30203

TBK1 antibody

TBK1 Mouse 464.8 Purified Bov, Can, Hu, Por, Rb, Rt WB
0.1 ml / €390.00
  IgG2a   GeneTex Inc.
 
BM5518P

TER ATPase / VCP antibody

TER ATPase / VCP Mouse 58.13.3 Purified Hu, Rb, Rt, Xen WB
50 µg / €310.00
  IgG1   Acris Antibodies GmbH
 
H00007415-M03

TER ATPase / VCP antibody

VCP monoclonal antibody (M03), clone 4A8. Western Blot analysis of VCP expression in HepG2 ( Cat # L019V1 ). Mouse 4A8 Purified Hu, Ms, Rt E, P, WB
0.1 mg / €340.00
  IgG2a   Abnova Taiwan Corp.
 
H00007415-M15

TER ATPase / VCP antibody

TER ATPase / VCP Mouse 2H5 Purified Hu E, WB
0.1 mg / €340.00
  IgG2a   Abnova Taiwan Corp.
 
H00007415-M18

TER ATPase / VCP antibody

VCP monoclonal antibody (M18), clone 2B2. Western Blot analysis of VCP expression in A-431(Cat # L015V1 ). Mouse 2B2 Purified Hu E, WB
0.1 mg / €340.00
  IgG1   Abnova Taiwan Corp.
 

18 Item(s)

per page
  • Pinterest
  • LinkedIn