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Antibodies to bcl-2 Protein Family - Product Review 04

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Introduction

Bcl-2 and related cytoplasmic proteins are key regulators of apoptosis, the cell suicide program critical for development, tissue homeostasis, and protection against pathogens.

Those most similar to Bcl-2 promote cell survival by inhibiting adapters needed for activation of the proteases (caspases) that dismantle the cell. More distant relatives instead promote apoptosis, apparently through mechanisms that include displacing the adapters from the pro-survival proteins. Thus, for many but not all apoptotic signals, the balance between these competing activities determines cell fate. Bcl-2 family members are essential for maintenance of major organ systems, and mutations affecting them are implicated in cancer.

At least 15 Bcl-2 family members have been identified in mammalian cells and several others in viruses. All members possess at least one of four conserved motifs known as Bcl-2 homology domains (BH1 to BH4). Most pro-survival members, which can inhibit apoptosis in the face of a wide variety of cytotoxic insults, contain at least BH1 and BH2, and those most similar to Bcl-2 have all four BH domains. The two pro-apoptotic subfamilies differ markedly in their relatedness to Bcl-2. Bax, Bak, and Bok (also called Mtd), which contain BH1, BH2, and BH3, resemble Bcl-2 fairly closely. In contrast, the seven other known mammalian "killers" possess only the central short (9 to 16 residue) BH3 domain; they are otherwise unrelated to any known protein, and only Bik and Blk are similar to each other. These "BH3 domain" proteins may well represent the physiological antagonists of the pro-survival proteins.

The Bcl2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with Bcl2 resulting in the enhancement of the anti-apoptotic effect of Bcl2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and cell death in a caspase-independent manner. Bad plays a critical role in the Bax-mediated apoptosis pathway by dimerizing with BclxL, causing the displacment of Bax. The displacement of Bax allows apoptosis to proceed. BclxS, a shorter version of BclxL (lacking amino acids 126-188), apparently utilizes a different pathway than Bax to induce cell death. Some research suggests that BclxS uses a novel mechanism for regulating caspase or it may use an alternate cell death effector pathway.





Antibody Tools for Detection of bcl-2 Family Members

Antibody products for detection of many of the above listed bcl-2 family members are available at Acris Antibodies. All antibodies are thoroughly tested for different immunological detection methods. For more informations like host, isotype, species cross-reactivity etc. see table 1 below. For pricing info and datasheet download use the links provided.



Figure 1: Staining of human tonsil using bcl-2 antibody DM130. Note cytoplasmic and memebrane staining of follicular cells. No staining of reactive cells in the germinal center

Figure 1: Staining of human tonsil using bcl-2 antibody DM130. Note cytoplasmic and memebrane staining of follicular cells. No staining of reactive cells in the germinal center

Figure 2: Staining of Hodgkin lymphoma using bcl-x antibody AM00285PU-N. Note staining of RS cells

Figure 2: Staining of Hodgkin lymphoma using bcl-x antibody AM00285PU-N. Note staining of RS cells

Figure 3: Staining of Hodgkin lymphoma using bcl-x antibody AP00535PU-N. Note staining of RS cells.

Figure 3: Staining of Hodgkin lymphoma using bcl-x antibody AP00535PU-N. Note staining of RS cells.

Figure 4: Staining of human tonsil using Mcl-1 antibody AM00277PU-N. Note nuclear staining of cells.

Figure 4: Staining of human tonsil using Mcl-1 antibody AM00277PU-N. Note nuclear staining of cells.

Figure 5: Staining of breast cancer using Bag-1 antibody DM335. Note nuclear and cytoplasmic staining of tumor cells

Figure 5: Staining of breast cancer using Bag-1 antibody DM335. Note nuclear and cytoplasmic staining of tumor cells

Figure 6: Staining of Hodgkin lymphoma using bax antibody DM246. Note membrane staining of RS cells

Figure 6: Staining of Hodgkin lymphoma using bax antibody DM246. Note membrane staining of RS cells

Figure 7: Staining of human tonsil using Bak antibody AP00539PU-N. Note cytoplasmic staining of mucosal epithelial cells.

Figure 7: Staining of human tonsil using Bak antibody AP00539PU-N. Note cytoplasmic staining of mucosal epithelial cells.

Figure 8: Western blot detection of human bim using SP7027P in K562 (left) and A549 cell lysates (right)

Figure 8: Western blot detection of human bim using SP7027P in K562 (left) and A549 cell lysates (right)

Figure 9: Western blot detection of human bmf using SP1414P in HepG2 (left) and 293 cell lysates (right)

Figure 9: Western blot detection of human bmf using SP1414P in HepG2 (left) and 293 cell lysates (right)

Available antibody panel to bcl-2 Protein Family

Anti- bcl-2, bcl-x, Bcl-xL , Mcl-1, Bag-1, bax, bak, Blk, Bim, Bad, Bmf, NOXA, PUMA-alpha, PUMA beta*



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434 products - FILTER YOUR RESULTS!

Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
AP05585PU-N

BAG1 (C-term) antibody

Western blot analysis in whole cell lysate from PC-3 human prostate cancer cells probed with Rabbit anti Human BAG1 (AP05585PU-N) at 1(A) and 2(B) µg/ml. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €380.00
  IgG   Acris Antibodies GmbH
 
AP05585PU-S

BAG1 (C-term) antibody

Western blot analysis in whole cell lysate from PC-3 human prostate cancer cells probed with Rabbit anti Human BAG1 (AP05585PU-N) at 1(A) and 2(B) µg/ml. Rabbit Purified Hu, Ms, Rt WB
50 µg / €250.00
  IgG   Acris Antibodies GmbH
 
AP05586PU-N

BAG1 (N-term) antibody

Western blot analysis of whole cell lysate from PC-3 human prostate cancer cells probed with Rabbit anti Human BAG1 (AP05586PU-N) at 1(A) and 2(B) µg/ml Rabbit Purified Hu, Ms WB
0.1 mg / €300.00
  IgG   Acris Antibodies GmbH
+1 additional image
 
AP05586PU-S

BAG1 (N-term) antibody

Western blot analysis of whole cell lysate from PC-3 human prostate cancer cells probed with Rabbit anti Human BAG1 (AP05586PU-N) at 1(A) and 2(B) µg/ml Rabbit Purified Hu, Ms WB
50 µg / €250.00
  IgG   Acris Antibodies GmbH
+1 additional image
 
AP07275PU-N

BAG1 antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €380.00
    Acris Antibodies GmbH
 
AP07658PU-N

BAG1 (N-term) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Adrenal. Rabbit Purified Hu, Ms P, WB
50 µg / €380.00
    Acris Antibodies GmbH
 
AP17147PU-N

BAG1 (C-term) antibody

Western blot analysis of BAG1 Antibody (C-term) (AP17147PU-N) in MDA-MB231 cell line lysates (35 µg/lane). BAG1 (arrow) was detected using the purified Pab. Rabbit Hu E, WB
0.1 mg / €330.00
    Acris Antibodies GmbH
 
DM335

BAG1 antibody

Formalin fixed paraffin embedded human breast carcinoma stained with BAG-1 antibody (DM335, DM335-05) Mouse 3.10G3E2 Purified Hu C, IP, P, WB
1 ml / €510.00
  IgG1   Acris Antibodies GmbH
+1 additional image
 
DM335-05

BAG1 antibody

Formalin fixed paraffin embedded human breast carcinoma stained with BAG-1 antibody (DM335, DM335-05) Mouse 3.10G3E2 Purified Hu C, IP, P, WB
0.5 ml / €350.00
  IgG1   Acris Antibodies GmbH
+1 additional image
 
GTX17855

BAG1 antibody

BAG1 Mouse SPM232 Purified Hu P
7 ml / €330.00
  IgG1   GeneTex Inc.
 
GTX22355

BAG1 antibody

BAG1 Mouse 3.10G3E2 Purified Hu C, IP, P, WB
0.5 ml / €420.00
  IgG1   GeneTex Inc.
 
GTX27976

BAG1 antibody

BAG1 Rabbit Purified Hu, Ms, Rt C, P, WB
1 ml / €390.00
  IgG   GeneTex Inc.
 
GTX102433

BAG1 antibody

BAG1 Rabbit Aff - Purified Hu ICC/IF, WB
0.1 ml / €300.00
    GeneTex Inc.
 
GTX112873

BAG1 antibody

BAG1 Rabbit Aff - Purified Hu P, WB
0.1 ml / €300.00
    GeneTex Inc.
 
H00000573-D01P

BAG1 antibody

Western Blot analysis of BAG1 expression in transfected 293T cell line (H00000573-T01) by BAG1 MaxPab polyclonal antibody. Lane 1: BAG1 transfected lysate(31.10 KDa). Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €360.00
    Abnova Taiwan Corp.
+1 additional image
 
PAB0328

BAG1 antibody

Formalin-fixed, paraffin-embedded section of human colon stained for Bag1. Using Bag1 polyclonal antibody ( Cat # PAB0327 ) at 1 : 2000. Hematoxylin-eosin counterstain. Rabbit Bov, Can, Hu, Ms, Rt IP, P
50 µl / €330.00
    Abnova Taiwan Corp.
+1 additional image
 
PAB0809

BAG1 antibody

BAG1 Rabbit Hu, Ms, Rt C, WB
0.2 mg / €330.00
    Abnova Taiwan Corp.
 
DB004P

BAG1 Control Peptide

BAG1 Purified
0.1 mg / €100.00
    Delta Biolabs LLC
 
AP15913PU-N

BAG2 (C-term) antibody

AP15913PU-N (0.3 µg/ml) staining of Hela lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €270.00
    Acris Antibodies GmbH
 
SP7411

BAG2 antibody

Immunocytochemistry/Immunofluorescence using BAG2 antibody (SP7411). Immunofluorescence confocal microscopy of BAG-2 using SP7411 at 1/2000. HeLa cells stably transfected with Myc-CHIP (carboxyl terminus of Hsp70-interacting protein) and growing on coverslips were left untreated (A) or heat shocked at 42 degrees for 30 min (B). Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG-2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock. Please refer to Dai et al (2005) for additional information on HeLa cells stably transfected with Myc-CHIP. Rabbit Serum Hu C, IP, P, WB
50 µl / €270.00
    Acris Antibodies GmbH
+1 additional image
 

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