Antibodies to Cytokeratin Proteins - Product Review 09

Introduction

Intermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells. Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells.

Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin 1 (68 kDa) is a secondary type II keratin expressed in cornified epithelia. Cytokeratin 4 (59 kDa) is the secondary type II keratin expressed in non-cornified stratified squamous epithelia.

Cytokeratin 5 (58 kDa) is the primary type II keratin in stratified epithelia while cytokeratin type 8 (52 kDa) is the primary type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a ’hyperproliferation‘ cytokeratin expressed in tissues with natural or pathological high turnover. The deduced human cytokeratin 7 protein contains 469 amino acids and has a calculated molecular mass of 51.4 kD. Cytokeratin 7 shares 82% amino acid identity with mouse cytokeratin 7 and 76% identity with rat kangaroo cytokeratin 7.

Western blot analysis of mouse tissues detected cytokeratin 7 expression in bladder, but not in epidermis. Cytokeratin 7 expression was found to be restricted to ducts and specific glands of simple epithelial tissues; examples included isolated cells in the acini of mammary gland, cells of lung alveoli and bronchiolar epithelium, kidney collecting duct, and Brunner glands of the duodenum.

Cytokeratins 10, 13, and 18 are members of the type I acidic subfamily. Cytokeratin 10 (56 kDa) is the secondary type I keratin expressed in cornified epithelia. Cytokeratin 13 (54 kDa) is the secondary type I keratin expressed in non-cornified stratified squamous epithelia. Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells.

Cytokeratin 19 (40 kDa) is a type I keratin which can be expressed in both simple and in broad cells of stratifying epithelia at specific sites. Cytokeratin 20 has an apparent molecular mass of about 46 kD. Antibodies raised against cytokeratin 20 decorated typical cytokeratin fibril arrays in normal and transformed intestinal cells.

Immunocytochemical analysis of several human and rat tissues showed that cytokeratin 20 is a prominent component of intestinal and gastric foveolar epithelium, urothelial umbrella cells, and Merkel cells of epidermis. Sparse positive epithelial cells were noted in the thymus, bronchus, gall bladder, and prostate gland. Monoclonal anti-cytokeratin antibodies are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification.

Antibody Tools for Detection of Cytokeratins

Acris Antibodies offers one of the worlds most comprehensive antibody product ranges for detection of all important keratins. These antibodies are suitable for immunohistochemical staining procedures (see picture gallery below) and / or other applications like Western blotting.

Picture Gallery

Staining of human skin using Pan Cytokeratin Marker AE1+AE3 cat# BM5001. Note cytoplasmic staining of epithelial cells

Staining of human skin using Pan Cytokeratin Marker AE1+AE3 cat# BM5001. Note cytoplasmic staining of epithelial cells

Staining of squamous cell lung carcinoma using pan Cytokeratin marker antibody Lu-5 cat# BM4068. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using pan Cytokeratin marker antibody Lu-5 cat# BM4068. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 20 antibody Ke20.8 cat# DM123. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 20 antibody Ke20.8 cat# DM123. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using acidic Cytokeratin marker antibody AE1 cat# BM5000 Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using acidic Cytokeratin marker antibody AE1 cat# BM5000 Note cytoplasmic staining of tumor cells.

Staining of human prostata using pan basic Cytokeratin marker antibody DE-SQ cat# DM275. Note staining of basal epithelial cells

Staining of human prostata using pan basic Cytokeratin marker antibody DE-SQ cat# DM275. Note staining of basal epithelial cells

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human tonsil using anti Cytokeratin 4 antibody 6B10 cat# BM559. Note cytoplasmic staining of mucosal epithelial cells

Staining of human tonsil using anti Cytokeratin 4 antibody 6B10 cat# BM559. Note cytoplasmic staining of mucosal epithelial cells

Staining of human mesothelioma using anti Cytokeratin 5, 6, 18 antibody LP34 cat# DM051. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6, 18 antibody LP34 cat# DM051. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6 antibody D5/16B4 cat# DM362. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6 antibody D5/16B4 cat# DM362. Note cytoplasmic staining of tumor cells

Staining of human lung carcinoma using anti Cytokeratin 5, 8 antibody C50 cat# DM182. Note cytoplasmic staining of tumor cells.

Staining of human lung carcinoma using anti Cytokeratin 5, 8 antibody C50 cat# DM182. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 6 antibody LHK6B cat# DM271. Note cytoplasmic staining of epiderrmal cells.

Staining of human skin using anti Cytokeratin 6 antibody LHK6B cat# DM271. Note cytoplasmic staining of epiderrmal cells.

Staining of human breast carcinoma using anti Cytokeratin 18 antibody DA-7 cat# SM3044. Note cytoplasmic staining of tumor cells.

Staining of human breast carcinoma using anti Cytokeratin 18 antibody DA-7 cat# SM3044. Note cytoplasmic staining of tumor cells.

Staining of human ovarian carcinoma using anti Cytokeratin 7 antibody OVTL12/30 cat# DM057. Note cytoplasmic staining of tumor cells.

Staining of human ovarian carcinoma using anti Cytokeratin 7 antibody OVTL12/30 cat# DM057. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 8, 18 antibody 5D3 cat# DM189. Note cytoplasmic staining of epithelial cells in sweat glands

Staining of human skin using anti Cytokeratin 8, 18 antibody 5D3 cat# DM189. Note cytoplasmic staining of epithelial cells in sweat glands

Staing of human lung using anti Cytokeratin 8 antibody M20 cat# BM5045. Note cytoplasmic staining of bronchial epithelial cells

Staing of human lung using anti Cytokeratin 8 antibody M20 cat# BM5045. Note cytoplasmic staining of bronchial epithelial cells

Staining of human colon carcinoma using anti Cytokeratin 8 antibody 35betaH11 cat# DM054. Note cytoplasmic staining of tumor cells

Staining of human colon carcinoma using anti Cytokeratin 8 antibody 35betaH11 cat# DM054. Note cytoplasmic staining of tumor cells

Staining of human tonsil using anti Cytokeratin 10, 13 antibody DE-K13 cat# DM056. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human tonsil using anti Cytokeratin 10, 13 antibody DE-K13 cat# DM056. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human skin using anti Cytokeratin 10 antibody LHP1 cat# DM185. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody LHP1 cat# DM185. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody DE-K10 cat# DM055. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody DE-K10 cat# DM055. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human tonsil using anti Cytokeratin 13 antibody 1C7 cat# BM5047. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human tonsil using anti Cytokeratin 13 antibody 1C7 cat# BM5047. Note cytoplasmic staining of mucosal epithelial cells.

Staining of rat breast carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staining of rat breast carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using anti Cytokeratin 14 antibody LL002 cat# SM1359. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using anti Cytokeratin 14 antibody LL002 cat# SM1359. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 15 antibody LHK15 cat# DM305. Note cytoplasmic staining of epithelial cells in external rod sheets of hair follicle

Staining of human skin using anti Cytokeratin 15 antibody LHK15 cat# DM305. Note cytoplasmic staining of epithelial cells in external rod sheets of hair follicle

Staing of human lung carcinoma using anti Cytokeratin 16 antibody LL025 cat# SM1471. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 16 antibody LL025 cat# SM1471. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

 

Available Antibody Panel to Cytokeratins

Anti- Cytokeratin (pan), anti- Cytokeratin-1, -3, -4, -5, -6, -7, -8, -9, -10, -13, -14, -15, -16, -17, -18, -19, and -20 antibody

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49 products - FILTER YOUR RESULTS!

Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
AM10029PU-N

acidic Cytokeratin antibody

acidic Cytokeratin Mouse IgG1 AE1 Purified Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt C, ICC/IF, P

Regular Price: 1 ml / €280.00

Special Price: 1 ml / €182.00

    Acris Antibodies GmbH
AM10029PU-S

acidic Cytokeratin antibody

acidic Cytokeratin Mouse IgG1 AE1 Purified Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt C, ICC/IF, P
0.5 ml / €220.00
    Acris Antibodies GmbH
AM10030PU-N

basic Cytokeratin antibody

basic Cytokeratin Mouse IgG1 AE3 Purified Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt C, ICC/IF, P
1 ml / €280.00
    Acris Antibodies GmbH
AM10030PU-S

basic Cytokeratin antibody

basic Cytokeratin Mouse IgG1 AE3 Purified Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt C, ICC/IF, P
0.5 ml / €220.00
    Acris Antibodies GmbH
BM5152P

basic Cytokeratin antibody

basic Cytokeratin Mouse IgG2a Ks pan 1-8 Purified Amph, Bov, Hu, Rt C, ICC/IF, WB
50 µg / €290.00
    Acris Antibodies GmbH
NB120-9287

basic Cytokeratin antibody

basic Cytokeratin Mouse IgG AE3 Aff - Purified Bov, Chk, Hu, Mky, Ms, Rb, Rt C, ICC/IF, P, WB
0.1 mg / €250.00
    Novus Biologicals Inc.
GTX23117

Cytokeratin 1 antibody

Cytokeratin 1 Mouse IgG1 AE1 Liquid Bov, Hu, Mky, Ms, Rb, Rt P
0.5 ml / €300.00
    GeneTex Inc.
DM052

Cytokeratin 5+6+8+17+19 antibody

Formalin fixed paraffin embedded human tonsil stained with Cytokeratin Antibody (Clone MNF116). Mouse IgG1 MNF116 Supernatant Bov, Eq, GP, Hu, Ms, Por, Rt P
1 ml / €380.00
    Acris Antibodies GmbH
DM052-05

Cytokeratin 5+6+8+17+19 antibody

Formalin fixed paraffin embedded human tonsil stained with Cytokeratin Antibody (Clone MNF116). Mouse IgG1 MNF116 Supernatant Bov, Eq, GP, Hu, Ms, Por, Rt P
0.5 ml / €270.00
    Acris Antibodies GmbH
DM051

Cytokeratin 5+6+18 antibody

Formalin fixed paraffin embedded human skin stained with Cytokeratin 6/18 antibody (Cat.#DM051). Mouse IgG1 LP34 Supernatant Bov, Hu, Rt P
1 ml / €380.00
    Acris Antibodies GmbH
DM051-05

Cytokeratin 5+6+18 antibody

Formalin fixed paraffin embedded human skin stained with Cytokeratin 6/18 antibody (Cat.#DM051). Mouse IgG1 LP34 Supernatant Bov, Hu, Rt P
0.5 ml / €270.00
    Acris Antibodies GmbH
BM5042

Cytokeratin 5 (+ KRT8) antibody

Cytokeratin 5 Mouse IgG1 C22 Purified Amph, Bov, Hu, Hydra, Ms, Por C, ICC/IF, P, WB
50 µg / €290.00
    Acris Antibodies GmbH
BM5042B

Cytokeratin 5 (+ KRT8) antibody

Cytokeratin 5 Mouse IgG1 C22 Biotin Amph, Bov, Hu, Hydra, Ms, Por C, E, F, ICC/IF, P
0.25 ml / €330.00
    Acris Antibodies GmbH
BM5042F

Cytokeratin 5 (+ KRT8) antibody

Cytokeratin 5 Mouse IgG1 C22 FITC Amph, Bov, Hu, Hydra, Ms, Por C, F, ICC/IF, P
0.25 ml / €330.00
    Acris Antibodies GmbH
NB500-348

Cytokeratin 5 (+ KRT18) antibody

Western Blot: Cytokeratin 5+18 Antibody (C-50) [NB500-348] - Fig. 1. Western Blotting analysis of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. Lane 1: MW standard Lane 2: immunostaining with anti-Cytokeratin 5+18 (C-50) Lane 3: immunostaining with Isotype mouse IgG1 control (PPV-06). Mouse IgG1 C-50 Aff - Purified Bov, Can, Hst, Hu, Ms, Por, Rt, Sh ICC/IF, IP, P, WB
0.1 mg / €310.00
    Novus Biologicals Inc.
BM5154

Cytokeratin 7 antibody

Cytokeratin 7 Mouse IgG1 Ks 7.18 Purified Bov, Hu, Por, Sh C, ICC/IF, P, WB
50 µg / €270.00
    Acris Antibodies GmbH
SM3046P

Cytokeratin 7 (+ KRT17) antibody

Detection of Cytokeratin 7+17 in HeLa cell lysate with monoclonal  anti-Cytokeratin 7+17  Antibody Cat.-No SM3046P (Clone C-46). Mouse IgG1 C-46 Purified Bov, Hu C, ICC/IF, IP, P, WB
0.1 mg / €200.00
    Acris Antibodies GmbH
BM562S

Cytokeratin 8 antibody

Detection of cytokeratin 8 in HeLa cell lysate by mouse monoclonal antibody C-51. Mouse IgG1 C-51 Purified Bov, Hu, Por, Sh C, ICC/IF, IP, P, WB
0.1 mg / €200.00
    Acris Antibodies GmbH
SM3079P

Cytokeratin 8 antibody

Formalin-Fixed, Paraffin-Embedded Human prostate stained with Cytokeratin 8 antibody Cat.-No SM3079P followed by biotinylated Horse anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 C-43 Aff - Purified Bov, Hu, Por, Rb, Sh ICC/IF, IP, P, WB
0.1 mg / €200.00
    Acris Antibodies GmbH
NB500-349

Cytokeratin 8 antibody

Cytokeratin 8 Mouse IgG1 C-51 Aff - Purified Bov, Hu, Por, Sh ICC/IF, IP, P, WB
0.1 mg / €310.00
    Novus Biologicals Inc.

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