Antibodies to Cytokeratin Proteins - Product Review 09

Introduction

Intermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells. Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells.

Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin 1 (68 kDa) is a secondary type II keratin expressed in cornified epithelia. Cytokeratin 4 (59 kDa) is the secondary type II keratin expressed in non-cornified stratified squamous epithelia.

Cytokeratin 5 (58 kDa) is the primary type II keratin in stratified epithelia while cytokeratin type 8 (52 kDa) is the primary type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a ’hyperproliferation‘ cytokeratin expressed in tissues with natural or pathological high turnover. The deduced human cytokeratin 7 protein contains 469 amino acids and has a calculated molecular mass of 51.4 kD. Cytokeratin 7 shares 82% amino acid identity with mouse cytokeratin 7 and 76% identity with rat kangaroo cytokeratin 7.

Western blot analysis of mouse tissues detected cytokeratin 7 expression in bladder, but not in epidermis. Cytokeratin 7 expression was found to be restricted to ducts and specific glands of simple epithelial tissues; examples included isolated cells in the acini of mammary gland, cells of lung alveoli and bronchiolar epithelium, kidney collecting duct, and Brunner glands of the duodenum.

Cytokeratins 10, 13, and 18 are members of the type I acidic subfamily. Cytokeratin 10 (56 kDa) is the secondary type I keratin expressed in cornified epithelia. Cytokeratin 13 (54 kDa) is the secondary type I keratin expressed in non-cornified stratified squamous epithelia. Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells.

Cytokeratin 19 (40 kDa) is a type I keratin which can be expressed in both simple and in broad cells of stratifying epithelia at specific sites. Cytokeratin 20 has an apparent molecular mass of about 46 kD. Antibodies raised against cytokeratin 20 decorated typical cytokeratin fibril arrays in normal and transformed intestinal cells.

Immunocytochemical analysis of several human and rat tissues showed that cytokeratin 20 is a prominent component of intestinal and gastric foveolar epithelium, urothelial umbrella cells, and Merkel cells of epidermis. Sparse positive epithelial cells were noted in the thymus, bronchus, gall bladder, and prostate gland. Monoclonal anti-cytokeratin antibodies are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification.

Antibody Tools for Detection of Cytokeratins

Acris Antibodies offers one of the worlds most comprehensive antibody product ranges for detection of all important keratins. These antibodies are suitable for immunohistochemical staining procedures (see picture gallery below) and / or other applications like Western blotting.

Picture Gallery

Staining of human skin using Pan Cytokeratin Marker AE1+AE3 cat# BM5001. Note cytoplasmic staining of epithelial cells

Staining of human skin using Pan Cytokeratin Marker AE1+AE3 cat# BM5001. Note cytoplasmic staining of epithelial cells

Staining of squamous cell lung carcinoma using pan Cytokeratin marker antibody Lu-5 cat# BM4068. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using pan Cytokeratin marker antibody Lu-5 cat# BM4068. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 20 antibody Ke20.8 cat# DM123. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 20 antibody Ke20.8 cat# DM123. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using acidic Cytokeratin marker antibody AE1 cat# BM5000 Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using acidic Cytokeratin marker antibody AE1 cat# BM5000 Note cytoplasmic staining of tumor cells.

Staining of human prostata using pan basic Cytokeratin marker antibody DE-SQ cat# DM275. Note staining of basal epithelial cells

Staining of human prostata using pan basic Cytokeratin marker antibody DE-SQ cat# DM275. Note staining of basal epithelial cells

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human tonsil using anti Cytokeratin 4 antibody 6B10 cat# BM559. Note cytoplasmic staining of mucosal epithelial cells

Staining of human tonsil using anti Cytokeratin 4 antibody 6B10 cat# BM559. Note cytoplasmic staining of mucosal epithelial cells

Staining of human mesothelioma using anti Cytokeratin 5, 6, 18 antibody LP34 cat# DM051. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6, 18 antibody LP34 cat# DM051. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6 antibody D5/16B4 cat# DM362. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6 antibody D5/16B4 cat# DM362. Note cytoplasmic staining of tumor cells

Staining of human lung carcinoma using anti Cytokeratin 5, 8 antibody C50 cat# DM182. Note cytoplasmic staining of tumor cells.

Staining of human lung carcinoma using anti Cytokeratin 5, 8 antibody C50 cat# DM182. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 6 antibody LHK6B cat# DM271. Note cytoplasmic staining of epiderrmal cells.

Staining of human skin using anti Cytokeratin 6 antibody LHK6B cat# DM271. Note cytoplasmic staining of epiderrmal cells.

Staining of human breast carcinoma using anti Cytokeratin 18 antibody DA-7 cat# SM3044. Note cytoplasmic staining of tumor cells.

Staining of human breast carcinoma using anti Cytokeratin 18 antibody DA-7 cat# SM3044. Note cytoplasmic staining of tumor cells.

Staining of human ovarian carcinoma using anti Cytokeratin 7 antibody OVTL12/30 cat# DM057. Note cytoplasmic staining of tumor cells.

Staining of human ovarian carcinoma using anti Cytokeratin 7 antibody OVTL12/30 cat# DM057. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 8, 18 antibody 5D3 cat# DM189. Note cytoplasmic staining of epithelial cells in sweat glands

Staining of human skin using anti Cytokeratin 8, 18 antibody 5D3 cat# DM189. Note cytoplasmic staining of epithelial cells in sweat glands

Staing of human lung using anti Cytokeratin 8 antibody M20 cat# BM5045. Note cytoplasmic staining of bronchial epithelial cells

Staing of human lung using anti Cytokeratin 8 antibody M20 cat# BM5045. Note cytoplasmic staining of bronchial epithelial cells

Staining of human colon carcinoma using anti Cytokeratin 8 antibody 35betaH11 cat# DM054. Note cytoplasmic staining of tumor cells

Staining of human colon carcinoma using anti Cytokeratin 8 antibody 35betaH11 cat# DM054. Note cytoplasmic staining of tumor cells

Staining of human tonsil using anti Cytokeratin 10, 13 antibody DE-K13 cat# DM056. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human tonsil using anti Cytokeratin 10, 13 antibody DE-K13 cat# DM056. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human skin using anti Cytokeratin 10 antibody LHP1 cat# DM185. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody LHP1 cat# DM185. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody DE-K10 cat# DM055. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody DE-K10 cat# DM055. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human tonsil using anti Cytokeratin 13 antibody 1C7 cat# BM5047. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human tonsil using anti Cytokeratin 13 antibody 1C7 cat# BM5047. Note cytoplasmic staining of mucosal epithelial cells.

Staining of rat breast carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staining of rat breast carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using anti Cytokeratin 14 antibody LL002 cat# SM1359. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using anti Cytokeratin 14 antibody LL002 cat# SM1359. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 15 antibody LHK15 cat# DM305. Note cytoplasmic staining of epithelial cells in external rod sheets of hair follicle

Staining of human skin using anti Cytokeratin 15 antibody LHK15 cat# DM305. Note cytoplasmic staining of epithelial cells in external rod sheets of hair follicle

Staing of human lung carcinoma using anti Cytokeratin 16 antibody LL025 cat# SM1471. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 16 antibody LL025 cat# SM1471. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

 

Available Antibody Panel to Cytokeratins

Anti- Cytokeratin (pan), anti- Cytokeratin-1, -3, -4, -5, -6, -7, -8, -9, -10, -13, -14, -15, -16, -17, -18, -19, and -20 antibody

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Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
NB110-60501

Cytokeratin 1 antibody

Cytokeratin 1 Mouse Ascites Hu C, E, P, WB
0.1 ml / €330.00
    Novus Biologicals Inc.
NB110-89450

Cytokeratin 1 antibody

Western Blot: CK1 Antibody (PMC01) [NB110-89450] - truncated CK1 recombinant protein. Mouse PMC01 Ascites Hu C, E, P, WB
0.1 ml / €330.00
    Novus Biologicals Inc.
16572-1-AP

Cytokeratin 4 antibody

Immunohistochemical of  paraffin-embedded human cervix using 16572-1-AP(KRT4 antibody) at dilution of 1:100 (under 10x lens) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.15 ml / €250.00
    Proteintech Group, Inc.
AP06203PU-N

Cytokeratin 5 antibody

Western blot(WB) analysis of Keratin 5/  antibody (Cat.-No.: AP06203PU-N)  in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
    Acris Antibodies GmbH
BM5042B

Cytokeratin 5 (+ KRT8) antibody

Cytokeratin 5 Mouse IgG1 C22 Biotin Amph, Bov, Hu, Hydra, Ms, Por C, E, F, ICC/IF, P
0.25 ml / €330.00
    Acris Antibodies GmbH
H00003852-M08

Cytokeratin 5 antibody

Western Blot analysis of KRT5 expression in transfected 293T cell line by KRT5 monoclonal antibody (M08), clone 1A12.

Lane 1: KRT5 transfected lysate(62.4 KDa).
Lane 2: Non-transfected lysate.
Mouse IgG2a 1A12 Purified Hu E, WB
0.1 mg / €340.00
    Abnova Taiwan Corp.
10590-1-AP

Cytokeratin 6 antibody

Immunohistochemical of  paraffin-embedded human oesophagus using 10590-1-AP(KRT6A antibody) at dilution of 1:100 (under 10x lens) Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.15 ml / €250.00
    Proteintech Group, Inc.
15539-1-AP

Cytokeratin 7 antibody

Immunofluorescent analysis of Hela cells, using KRT7 antibody 15539-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.15 ml / €250.00
    Proteintech Group, Inc.
AM08174PU-N

Cytokeratin 8 (C-term) antibody

Cytokeratin 8 Mouse IgG2a SB37b Purified C, E, WB
0.1 mg / €370.00
    Acris Antibodies GmbH
BP5007

Cytokeratin 8 (+ KRT18) antibody

Cytokeratin 8 Guinea Pig Serum Bov, Chk, Hst, Hu, Ms, Rt, Xen C, E, P, WB
0.1 ml / €310.00
    Acris Antibodies GmbH
H00003856-M02

Cytokeratin 8 antibody

Western Blot analysis of KRT8 expression in transfected 293T cell line by KRT8 monoclonal antibody (M02), clone 3E3.

Lane 1: KRT8 transfected lysate(53.7 KDa).
Lane 2: Non-transfected lysate.
Mouse IgG2b 3E3 Purified Hu E, WB
0.1 mg / €340.00
    Abnova Taiwan Corp.
MAB5629

Cytokeratin 8 antibody

Cell Cytospin of the HCC38 breast carcinoma cell line was incubated with KRT8 monoclonal antibody, clone SB37b ( Cat # MAB5629 ). Positive cells were viewed by fluorescence microscopy. Mouse IgG2a SB37b Hu C, E, ICC/IF, WB
0.1 mg / €370.00
    Abnova Taiwan Corp.
10164-2-AP

Cytokeratin 13 antibody

Immunohistochemical of  paraffin-embedded human lung cancer using 10164-2-AP(KRT13 antibody) at dilution of 1:50 (under 10x lens) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.15 ml / €250.00
    Proteintech Group, Inc.
AP16852PU-N

Cytokeratin 13 (Isoform A) (C-term) antibody

Antibody (0.1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €270.00
    Acris Antibodies GmbH
NBP1-06047

Cytokeratin 13 antibody

Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody [NBP1-06047] -  (2.5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €370.00
    Novus Biologicals Inc.
H00003860-M05

Cytokeratin 13 antibody

Cytokeratin 13 Mouse IgG2a 4F5 Purified Hu E, WB
0.1 mg / €340.00
    Abnova Taiwan Corp.
PAB11444

Cytokeratin 13 antibody

KRT13 polyclonal antibody ( Cat # PAB11444 ) ( 0.1 µg/ml ) staining of human lung lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €330.00
    Abnova Taiwan Corp.
10143-1-AP

Cytokeratin 14 antibody

Immunofluorescent analysis of MCF-7 cells, using KRT14 antibody 10143-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.15 ml / €250.00
    Proteintech Group, Inc.
10137-1-AP

Cytokeratin 15 antibody

Immunohistochemical of  paraffin-embedded human breast cancer using 10137-1-AP(KRT15 antibody) at dilution of 1:50 (under 10x lens) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.15 ml / €250.00
    Proteintech Group, Inc.
17265-1-AP

Cytokeratin 16 antibody

A375 cells were subjected to SDS PAGE followed by western blot with 17265-1-AP(KRT16 antibody) at dilution of 1:500 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.15 ml / €250.00
    Proteintech Group, Inc.

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