Antibodies to Cytokeratin Proteins - Product Review 09

Introduction

Intermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells. Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells.

Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin 1 (68 kDa) is a secondary type II keratin expressed in cornified epithelia. Cytokeratin 4 (59 kDa) is the secondary type II keratin expressed in non-cornified stratified squamous epithelia.

Cytokeratin 5 (58 kDa) is the primary type II keratin in stratified epithelia while cytokeratin type 8 (52 kDa) is the primary type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a ’hyperproliferation‘ cytokeratin expressed in tissues with natural or pathological high turnover. The deduced human cytokeratin 7 protein contains 469 amino acids and has a calculated molecular mass of 51.4 kD. Cytokeratin 7 shares 82% amino acid identity with mouse cytokeratin 7 and 76% identity with rat kangaroo cytokeratin 7.

Western blot analysis of mouse tissues detected cytokeratin 7 expression in bladder, but not in epidermis. Cytokeratin 7 expression was found to be restricted to ducts and specific glands of simple epithelial tissues; examples included isolated cells in the acini of mammary gland, cells of lung alveoli and bronchiolar epithelium, kidney collecting duct, and Brunner glands of the duodenum.

Cytokeratins 10, 13, and 18 are members of the type I acidic subfamily. Cytokeratin 10 (56 kDa) is the secondary type I keratin expressed in cornified epithelia. Cytokeratin 13 (54 kDa) is the secondary type I keratin expressed in non-cornified stratified squamous epithelia. Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells.

Cytokeratin 19 (40 kDa) is a type I keratin which can be expressed in both simple and in broad cells of stratifying epithelia at specific sites. Cytokeratin 20 has an apparent molecular mass of about 46 kD. Antibodies raised against cytokeratin 20 decorated typical cytokeratin fibril arrays in normal and transformed intestinal cells.

Immunocytochemical analysis of several human and rat tissues showed that cytokeratin 20 is a prominent component of intestinal and gastric foveolar epithelium, urothelial umbrella cells, and Merkel cells of epidermis. Sparse positive epithelial cells were noted in the thymus, bronchus, gall bladder, and prostate gland. Monoclonal anti-cytokeratin antibodies are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification.

Antibody Tools for Detection of Cytokeratins

Acris Antibodies offers one of the worlds most comprehensive antibody product ranges for detection of all important keratins. These antibodies are suitable for immunohistochemical staining procedures (see picture gallery below) and / or other applications like Western blotting.

Picture Gallery

Staining of human skin using Pan Cytokeratin Marker AE1+AE3 cat# BM5001. Note cytoplasmic staining of epithelial cells

Staining of human skin using Pan Cytokeratin Marker AE1+AE3 cat# BM5001. Note cytoplasmic staining of epithelial cells

Staining of squamous cell lung carcinoma using pan Cytokeratin marker antibody Lu-5 cat# BM4068. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using pan Cytokeratin marker antibody Lu-5 cat# BM4068. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 20 antibody Ke20.8 cat# DM123. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 20 antibody Ke20.8 cat# DM123. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using acidic Cytokeratin marker antibody AE1 cat# BM5000 Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using acidic Cytokeratin marker antibody AE1 cat# BM5000 Note cytoplasmic staining of tumor cells.

Staining of human prostata using pan basic Cytokeratin marker antibody DE-SQ cat# DM275. Note staining of basal epithelial cells

Staining of human prostata using pan basic Cytokeratin marker antibody DE-SQ cat# DM275. Note staining of basal epithelial cells

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human tonsil using anti Cytokeratin 4 antibody 6B10 cat# BM559. Note cytoplasmic staining of mucosal epithelial cells

Staining of human tonsil using anti Cytokeratin 4 antibody 6B10 cat# BM559. Note cytoplasmic staining of mucosal epithelial cells

Staining of human mesothelioma using anti Cytokeratin 5, 6, 18 antibody LP34 cat# DM051. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6, 18 antibody LP34 cat# DM051. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6 antibody D5/16B4 cat# DM362. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6 antibody D5/16B4 cat# DM362. Note cytoplasmic staining of tumor cells

Staining of human lung carcinoma using anti Cytokeratin 5, 8 antibody C50 cat# DM182. Note cytoplasmic staining of tumor cells.

Staining of human lung carcinoma using anti Cytokeratin 5, 8 antibody C50 cat# DM182. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 6 antibody LHK6B cat# DM271. Note cytoplasmic staining of epiderrmal cells.

Staining of human skin using anti Cytokeratin 6 antibody LHK6B cat# DM271. Note cytoplasmic staining of epiderrmal cells.

Staining of human breast carcinoma using anti Cytokeratin 18 antibody DA-7 cat# SM3044. Note cytoplasmic staining of tumor cells.

Staining of human breast carcinoma using anti Cytokeratin 18 antibody DA-7 cat# SM3044. Note cytoplasmic staining of tumor cells.

Staining of human ovarian carcinoma using anti Cytokeratin 7 antibody OVTL12/30 cat# DM057. Note cytoplasmic staining of tumor cells.

Staining of human ovarian carcinoma using anti Cytokeratin 7 antibody OVTL12/30 cat# DM057. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 8, 18 antibody 5D3 cat# DM189. Note cytoplasmic staining of epithelial cells in sweat glands

Staining of human skin using anti Cytokeratin 8, 18 antibody 5D3 cat# DM189. Note cytoplasmic staining of epithelial cells in sweat glands

Staing of human lung using anti Cytokeratin 8 antibody M20 cat# BM5045. Note cytoplasmic staining of bronchial epithelial cells

Staing of human lung using anti Cytokeratin 8 antibody M20 cat# BM5045. Note cytoplasmic staining of bronchial epithelial cells

Staining of human colon carcinoma using anti Cytokeratin 8 antibody 35betaH11 cat# DM054. Note cytoplasmic staining of tumor cells

Staining of human colon carcinoma using anti Cytokeratin 8 antibody 35betaH11 cat# DM054. Note cytoplasmic staining of tumor cells

Staining of human tonsil using anti Cytokeratin 10, 13 antibody DE-K13 cat# DM056. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human tonsil using anti Cytokeratin 10, 13 antibody DE-K13 cat# DM056. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human skin using anti Cytokeratin 10 antibody LHP1 cat# DM185. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody LHP1 cat# DM185. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody DE-K10 cat# DM055. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody DE-K10 cat# DM055. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human tonsil using anti Cytokeratin 13 antibody 1C7 cat# BM5047. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human tonsil using anti Cytokeratin 13 antibody 1C7 cat# BM5047. Note cytoplasmic staining of mucosal epithelial cells.

Staining of rat breast carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staining of rat breast carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using anti Cytokeratin 14 antibody LL002 cat# SM1359. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using anti Cytokeratin 14 antibody LL002 cat# SM1359. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 15 antibody LHK15 cat# DM305. Note cytoplasmic staining of epithelial cells in external rod sheets of hair follicle

Staining of human skin using anti Cytokeratin 15 antibody LHK15 cat# DM305. Note cytoplasmic staining of epithelial cells in external rod sheets of hair follicle

Staing of human lung carcinoma using anti Cytokeratin 16 antibody LL025 cat# SM1471. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 16 antibody LL025 cat# SM1471. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

 

Available Antibody Panel to Cytokeratins

Anti- Cytokeratin (pan), anti- Cytokeratin-1, -3, -4, -5, -6, -7, -8, -9, -10, -13, -14, -15, -16, -17, -18, -19, and -20 antibody

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
DM182P

Cytokeratin 5 (+ KRT18) antibody

Immunohistochemistry staining of human prostate (paraffin section) using anti-cytokeratin 5+18 (clone C-50). Mouse IgG1 C-50 Purified Mam C, ICC/IF, IP, P, WB
0.1 mg / €200.00
  Acris Antibodies GmbH
NB500-348

Cytokeratin 5 (+ KRT18) antibody

Western Blot: Cytokeratin 5+18 Antibody (C-50) [NB500-348] - Fig. 1. Western Blotting analysis of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. Lane 1: MW standard Lane 2: immunostaining with anti-Cytokeratin 5+18 (C-50) Lane 3: immunostaining with Isotype mouse IgG1 control (PPV-06). Mouse IgG1 C-50 Aff - Purified Bov, Can, Hst, Hu, Ms, Por, Rt, Sh ICC/IF, IP, P, WB
0.1 mg / €310.00
  Novus Biologicals Inc.
MAB0853

Cytokeratin 5 (+ KRT18) antibody

Western blotting analysis of whole cell lysate of MCF - 7 human breast adenocarcinoma cell line. Lane 1 : MW standard. Lane 2 : Immunostaining with KRT5 / KRT18 monoclonal antibody, clone C - 50 ( Cat # MAB0853 ) . Lane 3 : Immunostaining with Isotype mouse IgG1 control. Mouse IgG1 C-50 Hst, Hu, Ms, Rt, Sh ICC/IF, IP, P, WB
0.1 mg / €340.00
  Abnova Taiwan Corp.
SM3046P

Cytokeratin 7 (+ KRT17) antibody

Detection of Cytokeratin 7+17 in HeLa cell lysate with monoclonal  anti-Cytokeratin 7+17  Antibody Cat.-No SM3046P (Clone C-46). Mouse IgG1 C-46 Purified Bov, Hu C, ICC/IF, IP, P, WB
0.1 mg / €200.00
  Acris Antibodies GmbH
NBP1-42574

Cytokeratin 7 antibody

Cytokeratin 7 Mouse IgG1 CK-7 Hu C, ICC/IF, IP, P, WB
0.1 mg / €340.00
  Novus Biologicals Inc.
BM562S

Cytokeratin 8 antibody

Detection of cytokeratin 8 in HeLa cell lysate by mouse monoclonal antibody C-51. Mouse IgG1 C-51 Purified Bov, Hu, Por, Sh C, ICC/IF, IP, P, WB
0.1 mg / €200.00
  Acris Antibodies GmbH
SM3079P

Cytokeratin 8 antibody

Formalin-Fixed, Paraffin-Embedded Human prostate stained with Cytokeratin 8 antibody Cat.-No SM3079P followed by biotinylated Horse anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 C-43 Aff - Purified Bov, Hu, Por, Rb, Sh ICC/IF, IP, P, WB
0.1 mg / €200.00
  Acris Antibodies GmbH
NB500-349

Cytokeratin 8 antibody

Cytokeratin 8 Mouse IgG1 C-51 Aff - Purified Bov, Hu, Por, Sh ICC/IF, IP, P, WB
0.1 mg / €310.00
  Novus Biologicals Inc.
NB500-350

Cytokeratin 8 antibody

Cytokeratin 8 Mouse IgG1 C-43 Aff - Purified Bov, Hu, Por, Rb, Sh ICC/IF, IP, P, WB
0.1 mg / €310.00
  Novus Biologicals Inc.
GTX22530

Cytokeratin 8 antibody

Cytokeratin 8 Mouse IgG1 C-43 Purified Bov, Hu, Por, Rb, Sh ICC/IF, IP, WB
0.1 mg / €300.00
  GeneTex Inc.
GTX22531

Cytokeratin 8 antibody

Cytokeratin 8 Mouse IgG1 C-51 Aff - Purified Bov, Hu, Por, Sh ICC/IF, IP, P, WB
0.1 mg / €320.00
  GeneTex Inc.
MAB0856

Cytokeratin 8 antibody

Cytokeratin 8 Mouse IgG1 C-43 Bov, Hu, Rb, Sh ICC/IF, IP, WB
0.1 mg / €340.00
  Abnova Taiwan Corp.
NB500-354

Cytokeratin 10 antibody

Immunohistochemistry: Cytokeratin 10 Antibody (VIK-10) [NB500-354] - Fig. 1. Immunohistochemistry staining of skin basaliom (paraffin-embedded sections) with anti-human Cytokeratin 10 (VIK-10). Mouse IgG1 VIK-10 Aff - Purified Hu ICC/IF, IP, P
0.1 mg / €310.00
  Novus Biologicals Inc.
GTX21421

Cytokeratin 10 antibody

Cytokeratin 10 Mouse IgG1 VIK-10 Purified Hu C, ICC/IF, IP, P, WB
0.1 mg / €320.00
  GeneTex Inc.
MAB3663

Cytokeratin 10 antibody

Immunohistochemistry staining of human skin basaliom ( paraffin-embedded sections ) with KRT10 monoclonal antibody, clone VIK-10 ( Cat # MAB3663 ). Mouse IgG1 VIK-10 Hu ICC/IF, IP, P
0.1 mg / €340.00
  Abnova Taiwan Corp.
SM1360PS

Cytokeratin 18 antibody

Immunohistochemistry staining of human colon (paraffin sections) using Cytokeratin 18 Antibody (clone DC-10). Mouse IgG1 DC-10 Purified Hu E, ICC/IF, IP, P, WB
0.1 mg / €200.00
  Acris Antibodies GmbH
SM3043P

Cytokeratin 18 antibody

Figure 1. Immunohistochemistry staining of kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04). Mouse IgG1 C-04 Purified Mam E, F, ICC/IF, IP, P, WB
0.1 mg / €210.00
  Acris Antibodies GmbH
SM3044P

Cytokeratin 18 antibody

Immunohistochemistry staining of Human colon (Paraffin Sections) using anti-Cytokeratin 18 antibody (clone DA-7). Mouse IgG1 DA-7 Purified Hu E, ICC/IF, IP, P, WB
0.1 mg / €200.00
  Acris Antibodies GmbH
NB120-7797

Cytokeratin 18 antibody

Cytokeratin 18 Mouse IgG1 DC 10 Purified Hu E, F, ICC/IF, IP, P, WB
0.1 mg / €310.00
  Novus Biologicals Inc.
NB500-306

Cytokeratin 18 antibody

Immunohistochemistry: Cytokeratin 18 Antibody (C-04) [NB500-306] - Fig. 1. Immunohistochemistry staining of kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04). Mouse IgG1 C-04 Aff - Purified Hu, Mam, Ms, Rt E, F, ICC/IF, IP, P, WB
0.1 mg / €310.00
  Novus Biologicals Inc.

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