Antibodies to TIMP Protein Family - Product Review 02

Introduction

The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tight-binding inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors.

The TIMP proteins share several structural features. These include the twelve cysteine residues in conserved regions of the molecule that form six disulfide bonds, essential for the formation of native conformations, and the N-terminal region that is necessary for inhibitory activities. The N-terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved off to produce the mature protein. The C-terminal regions are divergent, which may enhance the selectivity of inhibition and binding efficiency. Although the TIMP proteins share high homology, they may either be secreted extracellularly in soluble form (TIMP-1, TIMP-2 and TIMP-4) or bind to extracellular matrix components (TIMP-3). MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL1 beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e., a limited number of cell types can be induced to make these proteins).

Antibody Tools for the Detection of Tissue Inhibitors of Metalloproteinases (TIMPs)

Acris Antibodies offers a selection of monoclonal and polyclonal antibodies for immunological detection of various TIMP proteins. These antibodies can be used in applications like immunohistochemistry, immunoprecipitation or Western blotting.

TIMP-1

TIMP-1 was fully sequenced and clone by Carmichael, et al. It is produced and secreted in soluble form by a variety of cell types and is widely distributed throughout the body. It is an extensively glycosylated protein with a molecular mass of 28.5 kDa. TIMP-1 inhibits the active forms of MMPs, and complexes with the proform of MMP9. Like MMP9, TIMP-1 expression is sensitive to many factors. Increased synthesis of TIMP-1 is caused by a wide variety of reagents that include: TGF beta, EGF, PDGF, FGFb, PMA, alltransretinoic acid (RA), IL1 and IL11. The human TIMP-1 gene, about 0.9 kb, has the chromosomal location of Xp11.2311.4.

TIMP-2

TIMP-2 (also called CSC-21K) is a 21 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-2 shows a preference for MMP-2. TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

TIMP-3

TIMP-3 was first purified from chicken embryo fibroblasts and identified as ChIMP3. The human homologue of TIMP-3, was originally detected as an inducible serum protein in WI-38 fibroblasts. The TIMP-3 localization differs from that of the other three TIMPs, and is thought to be primarily deposited into the extracellular matrix (ECM). TIMP-3 is insoluble, binds to the ECM associated with a variety of cell types, and is widely distributed throughout the body. TIMP-3 shows 30% amino acid homology with TIMP-1 and 38% homology with TIMP-2. TIMP-3 has been shown to promote the detachment of transformed cells from ECM and to accelerate morphological changes associated with cell transformation. Furthermore, up-regulation of TIMP-3 has been associated with a block in the G1 phase of the cell cycle during differentiation of HL-60 leukemia cells. The human TIMP-3 gene has the chromosomal location of 22q12-22q13.

TIMP-4

TIMP-4 was identified by molecular cloning. TIMP-4 shows 37 % amino acid identity with TIMP-1 and 51 % homology with TIMP-2 and TIMP-3. TIMP-4 is secreted extracellularly, predominantly in heart and brain tissue. It may function in a tissue specific fashion in extracellular matrix (ECM) homeostasis. TIMP-4 has a strong inhibitory effect on the invasion of human breast cancer cells across reconstituted basement membranes suggesting that TIMP-4 may have an important role in inhibiting primary tumor growth and progression. The human TIMP-4 gene has the chromosomal location of 3p25.



Fig.1: Staining of human breast cancer for TIMP-1 using DP3131. Note cytplasmic staining of tumor cells.

Fig.1: Staining of human breast cancer for TIMP-1 using AP00544PU-N. Note cytoplasmic staining of tumor cells.

Fig.2: Staining of human breast cancer using SM1558P. Note cytplasmic staining of tumor cells.

Fig.2: Staining of human breast cancer using AM00492PU-N. Note cytoplasmic staining of tumor cells.

Fig.3: Staining of human pancreas cancer for TIMP-2 using DM318P. Note cytplasmic staining of tumor cells.

Fig.3: Staining of human pancreas cancer for TIMP-2 using AM00260PU-N. Note cytoplasmic staining of tumor cells.

Fig.4: Staining of human pancreas cancer for TIMP-2 using DP3132. Note cytplasmic staining of tumor cells.

Fig.4: Staining of human pancreas cancer for TIMP-2 using AP00537PU-N. Note cytoplasmic staining of tumor cells.

Fig.5: Staining of human breast cancer for TIMP-3 using DP3133. Note cytplasmic staining of tumor cells

Fig.5: Staining of human breast cancer for TIMP-3 using AP00548PU-N. Note cytoplasmic staining of tumor cells.

Fig.6: Staining of human breast cancer for TIMP-4 using DP3134. Note cytplasmic staining of tumor cells

Fig.6: Staining of human breast cancer for TIMP-4 using AP00549PU-N. Note cytoplasmic staining of tumor cells.

Available antibody panel to TIMP

TIMP-1 (TIMP1) antibody, TIMP-2 (TIMP2) antibody, TIMP-3 (TIMP3) antibody, TIMP-4 (TIMP4) antibody



  1. Quick-Filters:
  2. Cited (1)
  3. Highly Validated (1)
  4. Overnight Delivery (2)

18 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
AF2310

TIMP1 antibody

Staining of Human prostatic adenocarcinoma with TIMP1 Antibody Cat.-No AF2310 (Clone 7-6C1) at 2 µg/ml. Antibody positive in cytoplasma and in lumen secreted. 10x Mouse IgG1 7-6C1 Purified Bov, Hu E, P, WB
0.1 mg / €150.00
  Acris Antibodies GmbH
10753-1-AP

TIMP1 antibody

Immunohistochemistry of paraffin-embedded Ovary tumor using 10753-1-AP(TIMP1 Antibody) at Dilution 1:100 (under 10x lens) Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P
0.15 ml / €250.00
  Proteintech Group, Inc.
AP15821PU-N

TIMP1 antibody

Human colon ca. stained with Anti-TIMP-1 antibody (Cat#AP15821PU). Rabbit Purified Hu P, WB
1 ml / €340.00
  Acris Antibodies GmbH
AP15821PU-S

TIMP1 antibody

Human colon ca. stained with Anti-TIMP-1 antibody (Cat#AP15821PU). Rabbit Purified Hu P, WB
0.1 ml / €170.00
  Acris Antibodies GmbH
AF2610

TIMP1 antibody

Staining of FFPE Human prostatic cancer (20x and 40x) with TIMP1 Antibody Cat.-No AF2610 (Clone 147-6D11) at 2 µg/ml. Antibody positive in the cytoplasm of neoplastic epithelial cell. Mouse IgG1 147-6D11 Purified Hu P, WB
0.1 mg / €190.00
  Acris Antibodies GmbH
AM00259PU-N

TIMP1 antibody

TIMP1 Mouse IgG1 102B1 Purified Hu, Rt WB
0.1 mg / €410.00
  Acris Antibodies GmbH
AM00492PU-N

TIMP1 antibody

Formalin-fixed, paraffin-embedded human breast carcinoma stained with AM00492PU-N using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells. Mouse IgG1 102D1 Purified Hu P, WB
0.1 mg / €460.00
  Acris Antibodies GmbH
AM05381PU-N

TIMP1 antibody

Left: Immunohistochemical staining of human colon carcinoma tissue using TIMP-1 antibody. Right: Western blot using TIMP-1 antibody against recombinant human TIMP-1 proenzyme (400 ng/lane). Mouse IgG1 2A5 Purified Hu, Mky C, E, WB
0.2 mg / €300.00
  Acris Antibodies GmbH
AP17789PU-N

TIMP1 (N-term) antibody

Western blot analysis of TIMP1 Antibody (N-term) (AP17789PU-N) in CEM cell line lysates (35ug/lane). TIMP1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €340.00
  Acris Antibodies GmbH
BM741

TIMP1 antibody

TIMP1 Mouse IgG1 T2 Ig Fraction Hu C, E, FN, WB
0.1 mg / €650.00
  Acris Antibodies GmbH
BM782

TIMP1 antibody

TIMP1 Mouse IgG1 T3 Ig Fraction Hu C, E, WB
0.1 mg / €480.00
  Acris Antibodies GmbH
BP858

TIMP1 antibody

TIMP1 Rabbit Purified Bov, Eq, Ft, Hu, Rb, Rt, Sh C, E, P, WB
0.1 mg / €1,190.00
  Acris Antibodies GmbH
NB100-283

TIMP1 antibody

TIMP1 Rabbit Aff - Purified Bov, Hu, Sh WB
0.1 ml / €390.00
  Novus Biologicals Inc.
NB100-1916

TIMP1 antibody

Immunohistochemistry-Paraffin: TIMP1 Antibody [NB100-1916] - Immunohistochemical analysis of human colon carcinoma. Rabbit Aff - Purified Hu C, P
0.5 ml / €310.00
  Novus Biologicals Inc.
NB100-74551

TIMP1 antibody

TIMP1 Mouse IgG1 F31 P2 A5 Purified Hu P, WB
0.1 mg / €370.00
  Novus Biologicals Inc.
NB110-61000

TIMP1 antibody

Formalin fixed and paraffin embedded normal human placenta tissue is subjected to mouse anti human TIMP-1 Mouse IgG1 MM0034-4A16 Purified Hu P, WB
0.1 mg / €430.00
  Novus Biologicals Inc.
NBP1-42375

TIMP1 antibody

TIMP1 Mouse IgG1 3A3 Hu, Ms, Rb, Rt P, WB
0.2 ml / €310.00
  Novus Biologicals Inc.
NBP1-42376

TIMP1 antibody

TIMP1 Mouse IgG1 2A5 Hu P, WB
0.2 ml / €310.00
  Novus Biologicals Inc.

18 Item(s)

per page
  • Pinterest
  • LinkedIn