Antibodies to Matrix Metalloproteinases - Product Review 01

Introduction: Matrix metalloproteinases (MMPs) are a family of more than twenty secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins.

In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterize the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies (see also figure on the right).

 

All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer´s, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

 

Antibody Tools for Detection of Human Matrix Metalloproteinases

Antibody products for detection of most of the above listed MMPs are available at Acris Antibodies.

 

A lot of these antibodies are useful for staining paraffin embedded sections (see picture gallery below).

 

In addition many products can be used for Western blotting procedures, but also immunoprecipitation experiments and ELISA applications are possible.



Table 1: Matrix Metalloproteinases – Overview

Enzyme

Synonym

Mol. Weight*

Main substrates

MMP1

Collagenase I

54 kD

Collagen I, II, III, VII, X

MMP2

Gelatinase A

74 kD

Gelatin I, Collagen VI, V, VII, X

MMP3

Stromelysin 1

54 kD

Degrades Fibronectin, Laminin, Gelatins of type I, III, IV, V; Collagens III, IV, X, IX, cartilage proteoglycans.
Activates procollagenase.

MMP7

Martrilysin

30 kD

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin.
Activates procollagenase.

MMP8

Collagenase II

53 kD

Fibrillar type I, II, and III collagens

MMP9

Gelatinase B

78 kD

Gelatin types I, V and Collagen types IV, V

MMP10

Stromelysin 2

54 kD

Fibronectin, Gelatins of type I, III, IV, V

MMP11

Stromelysin 3

55 kD

Serine protease inhibitors (serpins)

MMP12

Macrophage Elastase

54 kD

Elastin

MMP13

Collagenase III

55 kD

Collagen I

MMP14

MT1-MMP

66 kD

Activates Progelatinase A, degrades many ECM components

MMP15

MT2-MMP

76 kD

Fibronectin, Tenascin, Nidogen, Aggrecan, Perlecan, Laminin

MMP16

MT3-MMP

70 kD

Collagen III, Fibronectin and more

MMP17

MT4-MMP

67 kD

Fibrin and some other ECM components

MMP19

RASI

57 kD

Aggrecan, COMP

MMP20

Enamelysin

54 kD

Amelogenin, Aggrecan, COMP

MMP21

 

65 kD

Alpha-1 Antitrypsin

MMP23

Femalysin

44 kD

Not identified so far. Expressed in ovary, testis.

MMP24

MT5-MMP

73 kD

Activates Progelatinase A, degrades Proteoglycans

MMP25

MT6-MMP

63 kD

Activates Progelatinase A

MMP26

Matrilysin 2

30 kD

Activates Progelatinase B, degrades other ECM components

MMP28

Epilysin

59 kD

Casein

* molecular weight of the unprocessed precursor





Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Available antibody panel to Matrix Metalloproteinases

Anti- Matrix Metalloproteinase-1 (MMP-1, Collagenase I), Matrix Metalloproteinase-2 (MMP-2, Gelatinase A), Matrix Metalloproteinase-3 (MMP-3, Stromelysin-1), Matrix Metalloproteinase-7 (MMP-7, Matrilysin) , Matrix Metalloproteinase-8 (MMP-8, Collagenase II), Matrix Metalloproteinase-9 (MMP-9, Gelatinase B), Matrix Metalloproteinase-10 (MMP-10, Stromelysin-2), Matrix Metalloproteinase-11 (MMP-11, Stromelysin-3), Matrix Metalloproteinase-12 (MMP-12, Metalloelastase), Matrix Metalloproteinase-13 (MMP-13, Collagenase III), Matrix Metalloproteinase-14 (MMP-14, MT1-MMP), Matrix Metalloproteinase-15 (MMP-15, MT2-MMP), Matrix Metalloproteinase-16 (MMP-16b, MT3-MMP), Matrix Metalloproteinase-17 (MMP-17, MT4-MMP), Matrix Metalloproteinase-19 (MMP-19, RASI), Matrix Metalloproteinase-20 (MMP-20, Enamelysin), Matrix Metalloproteinase-23 (MMP-23, Femalysin), Matrix Metalloproteinase-23B (MMP-23B), Matrix Metalloproteinase-24 (MMP-24, MT5-MMP), Matrix Metalloproteinase-26 (MMP-26, Matrilysin-2), Matrix Metalloproteinase-28 (MMP-28, Epilysin)



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48 products - FILTER YOUR RESULTS!

Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
AM05376PU-N

MMP-1 antibody

Left: Immunohistochemical staining using MMP-1 antibody on colon carcinoma tissue section. Right: Western blot using MMP-1 antibody on recombinant human MMP-1 proenzyme (400 ng/lane). Mouse IgG1 3B6 Purified Hu C, WB
0.2 mg / €300.00
    Acris Antibodies GmbH
AM08197PU-N

MMP-1 antibody

MMP-1 Mouse IgG2b SB12e Purified Hu C, E, P, WB
0.1 mg / €270.00
    Acris Antibodies GmbH
NBP1-28604

MMP-1 antibody

MMP-1 Mouse IgG2b SB12e Purified Hu C, E, P, WB
0.1 mg / €390.00
    Novus Biologicals Inc.
NBP1-28607

MMP-1 antibody

MMP-1 Mouse IgG1 SB13a Purified Hu C, E, P
50 µg / €310.00
    Novus Biologicals Inc.
GTX24043

MMP-1 antibody

MMP-1 Rabbit IgG Purified Hu C, P
0.5 ml / €390.00
    GeneTex Inc.
AF6810

MMP-2 antibody

Staining of FFPE Human skin (10x and 40x) with MMP-2 Antibody Cat.-No AF6810 (Clone 42-5D11) at 2 µg/ml. Antibody positive in cytoplasm of interstitial fibroblasts. Mouse IgG1 42-5D11 Purified Hu C, P, WB
0.1 mg / €300.00
    Acris Antibodies GmbH
AM05377PU-N

MMP-2 antibody

Left: Immunohistochemical staining of human oesophageal adenocarcinoma tissue using MMP-2 antibody. Right: Western blot using MMP-2 antibody on recombinant MMP-2 proenzyme (400 ng/lane). Mouse IgG1 4D3 Purified Hu C, E, WB
0.2 mg / €300.00
    Acris Antibodies GmbH
AM08198PU-N

MMP-2 antibody

MMP-2 Mouse IgG1 SB13a Purified Hu C, E, P
0.1 mg / €370.00
    Acris Antibodies GmbH
DM312

MMP-2 antibody

Formalin Fixed Paraffin Embedded Human placenta stained with MMP-2 antibody Cat.-No DM312 Mouse IgG1 A-Gel VC2 Purified Hu C, P
1 ml / €540.00
    Acris Antibodies GmbH
DM312-05

MMP-2 antibody

Formalin Fixed Paraffin Embedded Human placenta stained with MMP-2 antibody Cat.-No DM312 Mouse IgG1 A-Gel VC2 Purified Hu C, P
0.5 ml / €360.00
    Acris Antibodies GmbH
NB200-113

MMP-2 antibody

Western Blot: Metalloproteinase-2 Antibody [NB200-113] - Samples: Lane 1 - Human Recombinant MMP-2. Lane 2 - Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - NB 200-113, secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202. Mouse IgG1 MMP2/2C1 Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.1 ml / €330.00
    Novus Biologicals Inc.
NB200-113SS

MMP-2 antibody

MMP-2 Mouse IgG1 MMP2/2C1 Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
25 µl / €150.00
    Novus Biologicals Inc.
NB200-114

MMP-2 antibody

Immunohistochemistry-paraffin: MMP2 antibody [NB200-114] - Detection of MMP-2 staining in formalin-fixed paraffin-embedded human ovary tissue.  Primary antibody was used at 2.5 ug/ml with the avidin-biotin-peroxidase detection method. Mouse IgG1 MMP2/8B4 Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.1 ml / €340.00
    Novus Biologicals Inc.
NB200-114B

MMP-2 antibody

MMP-2 Mouse IgG1 MMP2/8B4 Purified Hu, Ms, Rt C, E, IP, P, WB
0.1 ml / €410.00
    Novus Biologicals Inc.
NB200-114H

MMP-2 antibody

MMP-2 Mouse IgG1 MMP2/8B4 Purified Hu, Ms, Rt C, E, IP, P, WB
0.1 ml / €410.00
    Novus Biologicals Inc.
NB200-114SS

MMP-2 antibody

MMP-2 Mouse IgG1 MMP2/8B4 Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
25 µl / €170.00
    Novus Biologicals Inc.
GTX21818

MMP-2 antibody

MMP-2 Mouse IgG1 A-Gel VC2 Purified Hu C, P
0.5 ml / €430.00
    GeneTex Inc.
GTX27033

MMP-2 antibody

MMP-2 IgG1 MMP2/2C1 Purified Hu, Ms, Rt C, E, IP, WB
50 µl / €390.00
    GeneTex Inc.
AF6610

MMP-3 antibody

Staining of Human prostatic adenocarcinoma with MMP-3 Antibody Cat.-No AF6610 (Clone 55-2A4) at 4 µg/ml. Antibody positive in cytoplasma and in lumen secreted. 10x Mouse IgG1 55-2A4 Purified Hu C, E, P, WB
0.1 mg / €300.00
    Acris Antibodies GmbH
AF7710

MMP-3 antibody

MMP-3 Mouse IgG1 148-1A3 Purified Rb C, E, P, WB

Regular Price: 0.1 mg / €360.00

Special Price: 0.1 mg / €252.00

    Acris Antibodies GmbH

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