Antibodies to Matrix Metalloproteinases - Product Review 01

Introduction: Matrix metalloproteinases (MMPs) are a family of more than twenty secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins.

In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterize the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies (see also figure on the right).

 

All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer´s, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

 

Antibody Tools for Detection of Human Matrix Metalloproteinases

Antibody products for detection of most of the above listed MMPs are available at Acris Antibodies.

 

A lot of these antibodies are useful for staining paraffin embedded sections (see picture gallery below).

 

In addition many products can be used for Western blotting procedures, but also immunoprecipitation experiments and ELISA applications are possible.



Table 1: Matrix Metalloproteinases – Overview

Enzyme

Synonym

Mol. Weight*

Main substrates

MMP1

Collagenase I

54 kD

Collagen I, II, III, VII, X

MMP2

Gelatinase A

74 kD

Gelatin I, Collagen VI, V, VII, X

MMP3

Stromelysin 1

54 kD

Degrades Fibronectin, Laminin, Gelatins of type I, III, IV, V; Collagens III, IV, X, IX, cartilage proteoglycans.
Activates procollagenase.

MMP7

Martrilysin

30 kD

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin.
Activates procollagenase.

MMP8

Collagenase II

53 kD

Fibrillar type I, II, and III collagens

MMP9

Gelatinase B

78 kD

Gelatin types I, V and Collagen types IV, V

MMP10

Stromelysin 2

54 kD

Fibronectin, Gelatins of type I, III, IV, V

MMP11

Stromelysin 3

55 kD

Serine protease inhibitors (serpins)

MMP12

Macrophage Elastase

54 kD

Elastin

MMP13

Collagenase III

55 kD

Collagen I

MMP14

MT1-MMP

66 kD

Activates Progelatinase A, degrades many ECM components

MMP15

MT2-MMP

76 kD

Fibronectin, Tenascin, Nidogen, Aggrecan, Perlecan, Laminin

MMP16

MT3-MMP

70 kD

Collagen III, Fibronectin and more

MMP17

MT4-MMP

67 kD

Fibrin and some other ECM components

MMP19

RASI

57 kD

Aggrecan, COMP

MMP20

Enamelysin

54 kD

Amelogenin, Aggrecan, COMP

MMP21

 

65 kD

Alpha-1 Antitrypsin

MMP23

Femalysin

44 kD

Not identified so far. Expressed in ovary, testis.

MMP24

MT5-MMP

73 kD

Activates Progelatinase A, degrades Proteoglycans

MMP25

MT6-MMP

63 kD

Activates Progelatinase A

MMP26

Matrilysin 2

30 kD

Activates Progelatinase B, degrades other ECM components

MMP28

Epilysin

59 kD

Casein

* molecular weight of the unprocessed precursor





Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Available antibody panel to Matrix Metalloproteinases

Anti- Matrix Metalloproteinase-1 (MMP-1, Collagenase I), Matrix Metalloproteinase-2 (MMP-2, Gelatinase A), Matrix Metalloproteinase-3 (MMP-3, Stromelysin-1), Matrix Metalloproteinase-7 (MMP-7, Matrilysin) , Matrix Metalloproteinase-8 (MMP-8, Collagenase II), Matrix Metalloproteinase-9 (MMP-9, Gelatinase B), Matrix Metalloproteinase-10 (MMP-10, Stromelysin-2), Matrix Metalloproteinase-11 (MMP-11, Stromelysin-3), Matrix Metalloproteinase-12 (MMP-12, Metalloelastase), Matrix Metalloproteinase-13 (MMP-13, Collagenase III), Matrix Metalloproteinase-14 (MMP-14, MT1-MMP), Matrix Metalloproteinase-15 (MMP-15, MT2-MMP), Matrix Metalloproteinase-16 (MMP-16b, MT3-MMP), Matrix Metalloproteinase-17 (MMP-17, MT4-MMP), Matrix Metalloproteinase-19 (MMP-19, RASI), Matrix Metalloproteinase-20 (MMP-20, Enamelysin), Matrix Metalloproteinase-23 (MMP-23, Femalysin), Matrix Metalloproteinase-23B (MMP-23B), Matrix Metalloproteinase-24 (MMP-24, MT5-MMP), Matrix Metalloproteinase-26 (MMP-26, Matrilysin-2), Matrix Metalloproteinase-28 (MMP-28, Epilysin)



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16 products - FILTER YOUR RESULTS!

Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
H00004312-D01P

MMP-1 antibody

Western Blot analysis of MMP1 expression in transfected 293T cell line (H00004312-T01) by MMP1 MaxPab polyclonal antibody.

Lane 1: MMP1 transfected lysate(54.00 KDa).
Lane 2: Non-transfected lysate.
Rabbit Purified Hu E(detection), P, WB
0.1 mg / €360.00
    Abnova Taiwan Corp.
AP15652PU-N

MMP-1 antibody

Human placenta stained with Anti-MMP-1 antibody Rabbit Purified Hu P, WB
1 ml / €340.00
    Acris Antibodies GmbH
AP15652PU-S

MMP-1 antibody

Human placenta stained with Anti-MMP-1 antibody Rabbit Purified Hu P, WB
0.1 ml / €170.00
    Acris Antibodies GmbH
AF9110

MMP-1 (active) antibody

Staining of FFPE Human prostate cancer (10x and 40x) with MMP-1 Antibody Cat.-No AF9110 (Clone 195-1H6) at 2 µg/ml. Antibody positive in the cytoplasm of epithelial cells and in stroma. Mouse IgG2b 195-1H6 Purified Hu P, WB
0.1 mg / €300.00
    Acris Antibodies GmbH
AM05376PU-N

MMP-1 antibody

Left: Immunohistochemical staining using MMP-1 antibody on colon carcinoma tissue section. Right: Western blot using MMP-1 antibody on recombinant human MMP-1 proenzyme (400 ng/lane). Mouse IgG1 3B6 Purified Hu C, WB
0.2 mg / €300.00
    Acris Antibodies GmbH
AM08197PU-N

MMP-1 antibody

MMP-1 Mouse IgG2b SB12e Purified Hu C, E, P, WB
0.1 mg / €270.00
    Acris Antibodies GmbH
AP00545PU-N

MMP-1 antibody

Formalin-fixed, paraffin-embedded human placenta stained with MMP-1 (AP00545PU-N) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts Rabbit Purified Hu P, WB
0.5 mg / €460.00
    Acris Antibodies GmbH
AP05837PU-N

MMP-1 antibody

MMP-1 Rabbit IgG Purified Hu E, IP, WB
0.1 mg / €400.00
    Acris Antibodies GmbH
AP05838PU-N

MMP-1 antibody

MMP-1 Rabbit IgG Purified Hu E, WB
0.15 mg / €480.00
    Acris Antibodies GmbH
NB600-1192

MMP-1 antibody

Formalin fixed paraffin embedded human placenta stained with MMP-1 antibody. Rabbit IgG Purified Hu P
0.5 ml / €370.00
    Novus Biologicals Inc.
NBP1-28604

MMP-1 antibody

MMP-1 Mouse IgG2b SB12e Purified Hu C, E, P, WB
0.1 mg / €390.00
    Novus Biologicals Inc.
NBP1-28607

MMP-1 antibody

MMP-1 Mouse IgG1 SB13a Purified Hu C, E, P
50 µg / €310.00
    Novus Biologicals Inc.
GTX24043

MMP-1 antibody

MMP-1 Rabbit IgG Purified Hu C, P
0.5 ml / €390.00
    GeneTex Inc.
H00004312-B01P

MMP-1 antibody

Western Blot analysis of MMP1 expression in transfected 293T cell line (H00004312-T01) by MMP1 MaxPab polyclonal antibody.

Lane 1: MMP1 transfected lysate(51.59 KDa).
Lane 2: Non-transfected lysate.
Mouse Purified Hu E(detection), WB
50 µg / €340.00
    Abnova Taiwan Corp.

Proteins

Catalog No. Species Pres. Purity Source  
H00004312-P01-10

MMP-1

MMP-1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €350.00
  Abnova Taiwan Corp.
H00004312-P01-25

MMP-1

MMP-1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €500.00
  Abnova Taiwan Corp.

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