Antibodies to Matrix Metalloproteinases - Product Review 01

Introduction: Matrix metalloproteinases (MMPs) are a family of more than twenty secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins.

In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterize the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies (see also figure on the right).

 

All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer´s, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

 

Antibody Tools for Detection of Human Matrix Metalloproteinases

Antibody products for detection of most of the above listed MMPs are available at Acris Antibodies.

 

A lot of these antibodies are useful for staining paraffin embedded sections (see picture gallery below).

 

In addition many products can be used for Western blotting procedures, but also immunoprecipitation experiments and ELISA applications are possible.



Table 1: Matrix Metalloproteinases – Overview

Enzyme

Synonym

Mol. Weight*

Main substrates

MMP1

Collagenase I

54 kD

Collagen I, II, III, VII, X

MMP2

Gelatinase A

74 kD

Gelatin I, Collagen VI, V, VII, X

MMP3

Stromelysin 1

54 kD

Degrades Fibronectin, Laminin, Gelatins of type I, III, IV, V; Collagens III, IV, X, IX, cartilage proteoglycans.
Activates procollagenase.

MMP7

Martrilysin

30 kD

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin.
Activates procollagenase.

MMP8

Collagenase II

53 kD

Fibrillar type I, II, and III collagens

MMP9

Gelatinase B

78 kD

Gelatin types I, V and Collagen types IV, V

MMP10

Stromelysin 2

54 kD

Fibronectin, Gelatins of type I, III, IV, V

MMP11

Stromelysin 3

55 kD

Serine protease inhibitors (serpins)

MMP12

Macrophage Elastase

54 kD

Elastin

MMP13

Collagenase III

55 kD

Collagen I

MMP14

MT1-MMP

66 kD

Activates Progelatinase A, degrades many ECM components

MMP15

MT2-MMP

76 kD

Fibronectin, Tenascin, Nidogen, Aggrecan, Perlecan, Laminin

MMP16

MT3-MMP

70 kD

Collagen III, Fibronectin and more

MMP17

MT4-MMP

67 kD

Fibrin and some other ECM components

MMP19

RASI

57 kD

Aggrecan, COMP

MMP20

Enamelysin

54 kD

Amelogenin, Aggrecan, COMP

MMP21

 

65 kD

Alpha-1 Antitrypsin

MMP23

Femalysin

44 kD

Not identified so far. Expressed in ovary, testis.

MMP24

MT5-MMP

73 kD

Activates Progelatinase A, degrades Proteoglycans

MMP25

MT6-MMP

63 kD

Activates Progelatinase A

MMP26

Matrilysin 2

30 kD

Activates Progelatinase B, degrades other ECM components

MMP28

Epilysin

59 kD

Casein

* molecular weight of the unprocessed precursor





Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Available antibody panel to Matrix Metalloproteinases

Anti- Matrix Metalloproteinase-1 (MMP-1, Collagenase I), Matrix Metalloproteinase-2 (MMP-2, Gelatinase A), Matrix Metalloproteinase-3 (MMP-3, Stromelysin-1), Matrix Metalloproteinase-7 (MMP-7, Matrilysin) , Matrix Metalloproteinase-8 (MMP-8, Collagenase II), Matrix Metalloproteinase-9 (MMP-9, Gelatinase B), Matrix Metalloproteinase-10 (MMP-10, Stromelysin-2), Matrix Metalloproteinase-11 (MMP-11, Stromelysin-3), Matrix Metalloproteinase-12 (MMP-12, Metalloelastase), Matrix Metalloproteinase-13 (MMP-13, Collagenase III), Matrix Metalloproteinase-14 (MMP-14, MT1-MMP), Matrix Metalloproteinase-15 (MMP-15, MT2-MMP), Matrix Metalloproteinase-16 (MMP-16b, MT3-MMP), Matrix Metalloproteinase-17 (MMP-17, MT4-MMP), Matrix Metalloproteinase-19 (MMP-19, RASI), Matrix Metalloproteinase-20 (MMP-20, Enamelysin), Matrix Metalloproteinase-23 (MMP-23, Femalysin), Matrix Metalloproteinase-23B (MMP-23B), Matrix Metalloproteinase-24 (MMP-24, MT5-MMP), Matrix Metalloproteinase-26 (MMP-26, Matrilysin-2), Matrix Metalloproteinase-28 (MMP-28, Epilysin)



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
10371-2-AP

MMP-1 antibody

Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10371-2-AP(MMP1 antibody) at dilution of 1:300 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, P, WB
0.15 ml / €250.00
  Proteintech Group, Inc.
PAB12708

MMP-1 antibody

Human placenta stained with MMP1 polyclonal antibody ( Cat # PAB12708 ) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Rabbit Hu, Ms, Rt E, IP, P, WB
0.1 mg / €330.00
  Abnova Taiwan Corp.
10373-2-AP

MMP-2 antibody

Immunohistochemical of  paraffin-embedded human breast cancer using 10373-2-AP(MMP2 antibody) at dilution of 1:50 (under 10x lens) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.15 ml / €250.00
  Proteintech Group, Inc.
NB200-193

MMP-2 antibody

Immunohistochemistry-frozen: MMP2 antibody [NB200-193] - Detection of MMP2 in a frozen injured rat nerve tissue section.  Stained using NB 200-193. Rabbit Aff - Purified Chk, Hu, Ms, Rt C, F, ICC/IF, P, WB
0.1 ml / €370.00
  Novus Biologicals Inc.
GTX104577

MMP-2 antibody

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX104577  diluted at 1:1000 Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €320.00
  GeneTex Inc.
Sample size available for 25µl / 99€ (read more…)
PAB12382

MMP-2 antibody

Detection of MMP2 in a frozen injured rat nerve tissue section using MMP2 polyclonal antibody ( Cat # PAB12382, 4 µg/ml ). Rabbit Chk, Hu, Ms, Rt C, E, P, WB
0.1 ml / €410.00
  Abnova Taiwan Corp.
AM00257AF-N

MMP-2 antibody

Formalin-fixed, paraffin-embedded human placenta stained with MMP-2 Ab (Cat. AM00257) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts. Mouse IgG1 CA-4001 Azide Free Hu, Ms, Rt FN, P, WB
0.1 mg / €510.00
  Acris Antibodies GmbH
AM00257PU-N

MMP-2 antibody

Formalin-fixed, paraffin-embedded human placenta stained with MMP-2 Ab (Cat. AM00257) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts. Mouse IgG1 CA-4001 Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €460.00
  Acris Antibodies GmbH
AP00546PU-N

MMP-2 antibody

MMP-2 Rabbit Purified Bov, Hu, Ms, Rt WB
0.5 mg / €460.00
  Acris Antibodies GmbH
AP06231PU-N

MMP-2 antibody

Western blot (WB) analysis of MMP-2 antibody (Cat.-No.: AP06231PU-N) in extracts from LOVO cells.
	. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
NB100-91876

MMP-2 antibody

Western Blot: MMP-2 Antibody [NB100-91876] - Western  blot  (WB)  analysis of MMP-2 (L638)  antibody in extracts from LOVO cells Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  Novus Biologicals Inc.
NB110-57218

MMP-2 antibody

Western Blot: MMP-2 Antibody (EP1183Y) [NB110-57218] - Western blot analysis on 1 ng recombinant protein using anti-MMP-2 (cat. # 110-57218), dilution 1:1000. Rabbit IgG EP1183Y Aff - Purified Hu, Ms, Rt WB
0.1 ml / €390.00
  Novus Biologicals Inc.
NB200-113

MMP-2 antibody

Western Blot: Metalloproteinase-2 Antibody [NB200-113] - Samples: Lane 1 - Human Recombinant MMP-2. Lane 2 - Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - NB 200-113, secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202. Mouse IgG1 MMP2/2C1 Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.1 ml / €330.00
  Novus Biologicals Inc.
NB200-113B

MMP-2 antibody

MMP-2 Mouse IgG1 MMP2/2C1 Biotin Hu, Ms, Rt C, E, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.
NB200-113G

MMP-2 antibody

MMP-2 Mouse IgG1 MMP2/2C1 DyLight488 Hu, Ms, Rt C, E, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.
NB200-113H

MMP-2 antibody

MMP-2 Mouse IgG1 MMP2/2C1 HRP Hu, Ms, Rt C, E, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.
NB200-113SS

MMP-2 antibody

MMP-2 Mouse IgG1 MMP2/2C1 Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
25 µl / €150.00
  Novus Biologicals Inc.
NB200-114

MMP-2 antibody

Immunohistochemistry-paraffin: MMP2 antibody [NB200-114] - Detection of MMP-2 staining in formalin-fixed paraffin-embedded human ovary tissue.  Primary antibody was used at 2.5 ug/ml with the avidin-biotin-peroxidase detection method. Mouse IgG1 MMP2/8B4 Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.1 ml / €340.00
  Novus Biologicals Inc.
NB200-114B

MMP-2 antibody

MMP-2 Mouse IgG1 MMP2/8B4 Purified Hu, Ms, Rt C, E, IP, P, WB
0.1 ml / €410.00
  Novus Biologicals Inc.
NB200-114G

MMP-2 antibody

MMP-2 Mouse IgG1 MMP2/8B4 DyLight488 Hu, Ms, Rt C, E, IP, P, WB
0.1 ml / €410.00
  Novus Biologicals Inc.

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