Antibodies to Matrix Metalloproteinases - Product Review 01

Introduction: Matrix metalloproteinases (MMPs) are a family of more than twenty secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins.

In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterize the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies (see also figure on the right).

 

All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer´s, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

 

Antibody Tools for Detection of Human Matrix Metalloproteinases

Antibody products for detection of most of the above listed MMPs are available at Acris Antibodies.

 

A lot of these antibodies are useful for staining paraffin embedded sections (see picture gallery below).

 

In addition many products can be used for Western blotting procedures, but also immunoprecipitation experiments and ELISA applications are possible.



Table 1: Matrix Metalloproteinases – Overview

Enzyme

Synonym

Mol. Weight*

Main substrates

MMP1

Collagenase I

54 kD

Collagen I, II, III, VII, X

MMP2

Gelatinase A

74 kD

Gelatin I, Collagen VI, V, VII, X

MMP3

Stromelysin 1

54 kD

Degrades Fibronectin, Laminin, Gelatins of type I, III, IV, V; Collagens III, IV, X, IX, cartilage proteoglycans.
Activates procollagenase.

MMP7

Martrilysin

30 kD

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin.
Activates procollagenase.

MMP8

Collagenase II

53 kD

Fibrillar type I, II, and III collagens

MMP9

Gelatinase B

78 kD

Gelatin types I, V and Collagen types IV, V

MMP10

Stromelysin 2

54 kD

Fibronectin, Gelatins of type I, III, IV, V

MMP11

Stromelysin 3

55 kD

Serine protease inhibitors (serpins)

MMP12

Macrophage Elastase

54 kD

Elastin

MMP13

Collagenase III

55 kD

Collagen I

MMP14

MT1-MMP

66 kD

Activates Progelatinase A, degrades many ECM components

MMP15

MT2-MMP

76 kD

Fibronectin, Tenascin, Nidogen, Aggrecan, Perlecan, Laminin

MMP16

MT3-MMP

70 kD

Collagen III, Fibronectin and more

MMP17

MT4-MMP

67 kD

Fibrin and some other ECM components

MMP19

RASI

57 kD

Aggrecan, COMP

MMP20

Enamelysin

54 kD

Amelogenin, Aggrecan, COMP

MMP21

 

65 kD

Alpha-1 Antitrypsin

MMP23

Femalysin

44 kD

Not identified so far. Expressed in ovary, testis.

MMP24

MT5-MMP

73 kD

Activates Progelatinase A, degrades Proteoglycans

MMP25

MT6-MMP

63 kD

Activates Progelatinase A

MMP26

Matrilysin 2

30 kD

Activates Progelatinase B, degrades other ECM components

MMP28

Epilysin

59 kD

Casein

* molecular weight of the unprocessed precursor





Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Available antibody panel to Matrix Metalloproteinases

Anti- Matrix Metalloproteinase-1 (MMP-1, Collagenase I), Matrix Metalloproteinase-2 (MMP-2, Gelatinase A), Matrix Metalloproteinase-3 (MMP-3, Stromelysin-1), Matrix Metalloproteinase-7 (MMP-7, Matrilysin) , Matrix Metalloproteinase-8 (MMP-8, Collagenase II), Matrix Metalloproteinase-9 (MMP-9, Gelatinase B), Matrix Metalloproteinase-10 (MMP-10, Stromelysin-2), Matrix Metalloproteinase-11 (MMP-11, Stromelysin-3), Matrix Metalloproteinase-12 (MMP-12, Metalloelastase), Matrix Metalloproteinase-13 (MMP-13, Collagenase III), Matrix Metalloproteinase-14 (MMP-14, MT1-MMP), Matrix Metalloproteinase-15 (MMP-15, MT2-MMP), Matrix Metalloproteinase-16 (MMP-16b, MT3-MMP), Matrix Metalloproteinase-17 (MMP-17, MT4-MMP), Matrix Metalloproteinase-19 (MMP-19, RASI), Matrix Metalloproteinase-20 (MMP-20, Enamelysin), Matrix Metalloproteinase-23 (MMP-23, Femalysin), Matrix Metalloproteinase-23B (MMP-23B), Matrix Metalloproteinase-24 (MMP-24, MT5-MMP), Matrix Metalloproteinase-26 (MMP-26, Matrilysin-2), Matrix Metalloproteinase-28 (MMP-28, Epilysin)



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17 products - FILTER YOUR RESULTS!

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
18165-1-AP

MMP-13 antibody

Immunohistochemical of  paraffin-embedded human breast cancer using 18165-1-AP(MMP13 antibody) at dilution of 1:50 (under 10x lens) Rabbit IgG Aff - Purified Hu, Ms, Rt, Ze E, P, WB
0.15 ml / €250.00
  Proteintech Group, Inc.
AF8910

MMP-13 antibody

MMP-13 Mouse IgG1 181-15A12 Purified Hu C, E, P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH
AP00230PU-N

MMP-13 antibody

Western Blot analysis of MMP13 expression with Jurkat Cell Lysate. Rabbit Aff - Purified Bov, Eq, Hu, Ms, Rb, Rt IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH
AP06226PU-N

MMP-13 antibody

Western blot (WB) analysis of MMP-13 antibody (Cat.-No.: AP06226PU-N) at 1/500 dilution Lane1:Hela whole cell lysate treated with IL-1β Lane2:sp2/0 whole cell lysate treated with IL-1β Lane3:H9C2 whole cell lysate treated with IL-1β Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
AP07212PU-N

MMP-13 antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms P, WB
50 µg / €380.00
  Acris Antibodies GmbH
AP13170PU-N

MMP-13 (C-term) antibody

Western blot analysis of MMP13(arrow) using rabbit polyclonal MMP13 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP13 gene (Lane 2) Rabbit Ig Purified Hu P, WB
0.4 ml / €340.00
  Acris Antibodies GmbH
BP617

MMP-13 antibody

MMP-13 Sheep Ig Fraction Hu, Ms, Rt WB
1 ml / €390.00
  Acris Antibodies GmbH
NB100-66244

MMP-13 antibody

MMP-13 Sheep IgG Aff - Purified Hu WB
1 ml / €500.00
  Novus Biologicals Inc.
NB100-91871

MMP-13 antibody

Western Blot: MMP-13 Antibody [NB100-91871] - Western  blot  (WB)  analysis of MMP-13 (L22)  antibody in extracts from LOVO cells Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €370.00
  Novus Biologicals Inc.
NB110-57215

MMP-13 antibody

Western Blot: MMP-13 Antibody (EP1263Y) [NB110-57215] - Western blot analysis on HeLa cell lysate using anti-MMP-13 (cat. # 110-57215), dilution 1:500. Rabbit IgG EP1263Y Aff - Purified Hu, Ms WB
0.1 ml / €390.00
  Novus Biologicals Inc.
NB110-60992

MMP-13 antibody

Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining using NB110-60992 (Proteinase K pretreatment is required). Mouse IgG1 MM0019-12E10 Purified Hu P, WB
0.1 mg / €430.00
  Novus Biologicals Inc.
GTX100665

MMP-13 antibody

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX100665 diluted at 1:1000 Rabbit Aff - Purified Hu P, WB
0.1 ml / €320.00
  GeneTex Inc.
Sample size available for 25µl / 99€ (read more…)
H00004322-B01P

MMP-13 antibody

Western Blot analysis of MMP13 expression in transfected 293T cell line (H00004322-T01) by MMP13 MaxPab polyclonal antibody.

Lane 1: MMP13 transfected lysate(51.81 KDa).
Lane 2: Non-transfected lysate.
Mouse Purified Hu E(detection), WB
50 µg / €340.00
  Abnova Taiwan Corp.
H00004322-D01P

MMP-13 antibody

Western Blot analysis of MMP13 expression in transfected 293T cell line (H00004322-T01) by MMP13 MaxPab polyclonal antibody.

Lane 1: MMP13 transfected lysate(53.80 KDa).
Lane 2: Non-transfected lysate.
Rabbit Purified Hu E(detection), WB
0.1 mg / €360.00
  Abnova Taiwan Corp.
PAB8846

MMP-13 antibody

Western blot analysis of Mmp13 expression in Jurkat cell lysate. Rabbit Hu, Rt IP, P, WB
0.1 mg / €410.00
  Abnova Taiwan Corp.
PAB12710

MMP-13 antibody

MMP-13 Rabbit Hu, Ms, Rt C, E, IP, WB
0.1 mg / €330.00
  Abnova Taiwan Corp.
29017

MMP-13 (Hinge Region) antibody

MMP-13 Rabbit Aff - Purified Hu C, E, IP, WB
0.15 mg / €420.00
  QED Bioscience Inc.

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