Antibodies to Matrix Metalloproteinases - Product Review 01

Introduction: Matrix metalloproteinases (MMPs) are a family of more than twenty secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins.

In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterize the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies (see also figure on the right).

 

All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer´s, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

 

Antibody Tools for Detection of Human Matrix Metalloproteinases

Antibody products for detection of most of the above listed MMPs are available at Acris Antibodies.

 

A lot of these antibodies are useful for staining paraffin embedded sections (see picture gallery below).

 

In addition many products can be used for Western blotting procedures, but also immunoprecipitation experiments and ELISA applications are possible.



Table 1: Matrix Metalloproteinases – Overview

Enzyme

Synonym

Mol. Weight*

Main substrates

MMP1

Collagenase I

54 kD

Collagen I, II, III, VII, X

MMP2

Gelatinase A

74 kD

Gelatin I, Collagen VI, V, VII, X

MMP3

Stromelysin 1

54 kD

Degrades Fibronectin, Laminin, Gelatins of type I, III, IV, V; Collagens III, IV, X, IX, cartilage proteoglycans.
Activates procollagenase.

MMP7

Martrilysin

30 kD

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin.
Activates procollagenase.

MMP8

Collagenase II

53 kD

Fibrillar type I, II, and III collagens

MMP9

Gelatinase B

78 kD

Gelatin types I, V and Collagen types IV, V

MMP10

Stromelysin 2

54 kD

Fibronectin, Gelatins of type I, III, IV, V

MMP11

Stromelysin 3

55 kD

Serine protease inhibitors (serpins)

MMP12

Macrophage Elastase

54 kD

Elastin

MMP13

Collagenase III

55 kD

Collagen I

MMP14

MT1-MMP

66 kD

Activates Progelatinase A, degrades many ECM components

MMP15

MT2-MMP

76 kD

Fibronectin, Tenascin, Nidogen, Aggrecan, Perlecan, Laminin

MMP16

MT3-MMP

70 kD

Collagen III, Fibronectin and more

MMP17

MT4-MMP

67 kD

Fibrin and some other ECM components

MMP19

RASI

57 kD

Aggrecan, COMP

MMP20

Enamelysin

54 kD

Amelogenin, Aggrecan, COMP

MMP21

 

65 kD

Alpha-1 Antitrypsin

MMP23

Femalysin

44 kD

Not identified so far. Expressed in ovary, testis.

MMP24

MT5-MMP

73 kD

Activates Progelatinase A, degrades Proteoglycans

MMP25

MT6-MMP

63 kD

Activates Progelatinase A

MMP26

Matrilysin 2

30 kD

Activates Progelatinase B, degrades other ECM components

MMP28

Epilysin

59 kD

Casein

* molecular weight of the unprocessed precursor





Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Available antibody panel to Matrix Metalloproteinases

Anti- Matrix Metalloproteinase-1 (MMP-1, Collagenase I), Matrix Metalloproteinase-2 (MMP-2, Gelatinase A), Matrix Metalloproteinase-3 (MMP-3, Stromelysin-1), Matrix Metalloproteinase-7 (MMP-7, Matrilysin) , Matrix Metalloproteinase-8 (MMP-8, Collagenase II), Matrix Metalloproteinase-9 (MMP-9, Gelatinase B), Matrix Metalloproteinase-10 (MMP-10, Stromelysin-2), Matrix Metalloproteinase-11 (MMP-11, Stromelysin-3), Matrix Metalloproteinase-12 (MMP-12, Metalloelastase), Matrix Metalloproteinase-13 (MMP-13, Collagenase III), Matrix Metalloproteinase-14 (MMP-14, MT1-MMP), Matrix Metalloproteinase-15 (MMP-15, MT2-MMP), Matrix Metalloproteinase-16 (MMP-16b, MT3-MMP), Matrix Metalloproteinase-17 (MMP-17, MT4-MMP), Matrix Metalloproteinase-19 (MMP-19, RASI), Matrix Metalloproteinase-20 (MMP-20, Enamelysin), Matrix Metalloproteinase-23 (MMP-23, Femalysin), Matrix Metalloproteinase-23B (MMP-23B), Matrix Metalloproteinase-24 (MMP-24, MT5-MMP), Matrix Metalloproteinase-26 (MMP-26, Matrilysin-2), Matrix Metalloproteinase-28 (MMP-28, Epilysin)



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
AF8410

MMP-14 antibody

Recombinant ΔMT1-MMP (lacking the TM domain) (expressed in CHO cells) stained with MMP-14 Antibody Cat.-No AF8410 (Clone 113-5B7) at 10 μg/ml (2 μg protein/Lane). HRP conjugated Goat anti Mouse IgG as Secondary antibody. Mouse IgG3 113-5B7 Purified Hu, Ms, Rb, Rt C, P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH
14552-1-AP

MMP-14 antibody

HepG2 cells were subjected to SDS PAGE followed by western blot with 14552-1-AP(MMP14 antibody) at dilution of 1:500 Rabbit IgG Aff - Purified Hu, Ms, Rt, Ze E, WB
0.15 ml / €250.00
  Proteintech Group, Inc.
AP15654PU-N

MMP-14 antibody

Human placenta stained
with anti-MMP-14 antibody Rabbit Purified Hu P, WB
1 ml / €340.00
  Acris Antibodies GmbH
AP15654PU-S

MMP-14 antibody

Human placenta stained
with anti-MMP-14 antibody Rabbit Purified Hu P, WB
0.1 ml / €170.00
  Acris Antibodies GmbH
AF8010

MMP-14 antibody

MMP-14 Mouse IgG1 114-1F2 Purified Hu WB
0.1 mg / €300.00
  Acris Antibodies GmbH
AF8610

MMP-14 antibody

MMP-14 Mouse IgG1 114-6G6 Purified Hu C, E, P, WB
0.1 mg / €360.00
  Acris Antibodies GmbH
AP06227PU-N

MMP-14 antibody

Western blot (WB) analysis of MMP-14 antibody (Cat.-No.: AP06227PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2:Mouse kidney tissue lysate Lane3:H9C2 whole cell lysate Lane4:SP2/0 whole cell lysate Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
AP07211PU-N

MMP-14 antibody

Lung, respiratory epithelium: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu P, WB
50 µg / €370.00
  Acris Antibodies GmbH
AP13171PU-N

MMP-14 (N-term) antibody

(LEFT)The anti-MMP14 N-term Antibody is used in Western blot to detect MMP14 in A375 lysate. (RIGHT)Western blot analysis of MMP14 (arrow) using rabbit polyclonal MMP14 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP14 gene (Lane 2) Rabbit Ig Purified Hu P, WB
0.4 ml / €340.00
  Acris Antibodies GmbH
BP618

MMP-14 antibody

MMP-14 Rabbit Aff - Purified Hu E, ICC/IF, WB
0.1 ml / €660.00
  Acris Antibodies GmbH
NB100-91872

MMP-14 antibody

Immunohistochemistry: MMP-14 Antibody [NB100-91872] - Immunohistochemistry  (IHC)  analyzes of MMP-14  (P509) antibody in paraffin-embedded human breast  carcinoma tissue Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  Novus Biologicals Inc.
NB110-57216

MMP-14 antibody

Western Blot: MMP-14 Antibody (EP1264Y) [NB110-57216] - Western blot analysis human spleen lysate using anti-MMP-14 (cat. # 110-57216), dilution 1:2000. Rabbit IgG EP1264Y Aff - Purified Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 ml / €430.00
  Novus Biologicals Inc.
NB110-60993

MMP-14 antibody

Immunohistochemistry-Paraffin: MMP14 Antibody (MM0027-9E10) [NB110-60993] - Human placenta (formalin fixed and paraffin embedded) section is subjected to IHC. Mouse IgG1 MM0027-9E10 Purified Hu P, WB
0.1 mg / €430.00
  Novus Biologicals Inc.
GTX11621

MMP-14 antibody

MMP-14 Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €360.00
  GeneTex Inc.
GTX23644

MMP-14 antibody

MMP-14 Rabbit IgG Purified Hu C, ICC/IF, P, WB
0.5 ml / €430.00
  GeneTex Inc.
GTX73117

MMP-14 antibody

MMP-14 Rabbit Aff - Purified Hu P, WB
0.5 ml / €330.00
  GeneTex Inc.
GTX100778

MMP-14 antibody

Sample (30 ug of whole cell lysate)  A: H1299
7.5% SDS PAGE  GTX100778 diluted at 1:1000 Rabbit Aff - Purified Hu P, WB
0.1 ml / €320.00
  GeneTex Inc.
Sample size available for 25µl / 99€ (read more…)
H00004323-D01P

MMP-14 antibody

Western Blot analysis of MMP14 expression in transfected 293T cell line (H00004323-T02) by MMP14 MaxPab polyclonal antibody.

Lane 1: MMP14 transfected lysate(65.90 KDa).
Lane 2: Non-transfected lysate.
Rabbit Purified Hu E(detection), WB
0.1 mg / €360.00
  Abnova Taiwan Corp.
PAB4782

MMP-14 antibody

The MMP14 polyclonal antibody ( Cat # PAB4782 ) is used in Western blot to detect MMP14 in A-375 cell lysate. Rabbit Hu E, P, WB
0.4 ml / €330.00
  Abnova Taiwan Corp.
PAB11703

MMP-14 antibody

Immunohistochemical staining of MMP14 on formalin fixed, paraffin embedded human placenta with MMP14 polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €330.00
  Abnova Taiwan Corp.

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