Antibodies to Matrix Metalloproteinases - Product Review 01
Introduction: Matrix metalloproteinases (MMPs) are a family of more than twenty secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins.
In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterize the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies (see also figure on the right).
All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer´s, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.
Antibody Tools for Detection of Human Matrix Metalloproteinases
Antibody products for detection of most of the above listed MMPs are available at Acris Antibodies.
A lot of these antibodies are useful for staining paraffin embedded sections (see picture gallery below).
In addition many products can be used for Western blotting procedures, but also immunoprecipitation experiments and ELISA applications are possible.
Table 1: Matrix Metalloproteinases – Overview
Enzyme | Synonym | Mol. Weight* | Main substrates |
MMP1 | Collagenase I | 54 kD | Collagen I, II, III, VII, X |
MMP2 | Gelatinase A | 74 kD | Gelatin I, Collagen VI, V, VII, X |
MMP3 | Stromelysin 1 | 54 kD | Degrades Fibronectin, Laminin, Gelatins of type I, III, IV, V; Collagens III, IV, X, IX, cartilage proteoglycans. |
MMP7 | Martrilysin | 30 kD | Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. |
MMP8 | Collagenase II | 53 kD | Fibrillar type I, II, and III collagens |
MMP9 | Gelatinase B | 78 kD | Gelatin types I, V and Collagen types IV, V |
MMP10 | Stromelysin 2 | 54 kD | Fibronectin, Gelatins of type I, III, IV, V |
MMP11 | Stromelysin 3 | 55 kD | Serine protease inhibitors (serpins) |
MMP12 | Macrophage Elastase | 54 kD | Elastin |
MMP13 | Collagenase III | 55 kD | Collagen I |
MMP14 | MT1-MMP | 66 kD | Activates Progelatinase A, degrades many ECM components |
MMP15 | MT2-MMP | 76 kD | Fibronectin, Tenascin, Nidogen, Aggrecan, Perlecan, Laminin |
MMP16 | MT3-MMP | 70 kD | Collagen III, Fibronectin and more |
MMP17 | MT4-MMP | 67 kD | Fibrin and some other ECM components |
MMP19 | RASI | 57 kD | Aggrecan, COMP |
MMP20 | Enamelysin | 54 kD | Amelogenin, Aggrecan, COMP |
MMP21 |
| 65 kD | Alpha-1 Antitrypsin |
MMP23 | Femalysin | 44 kD | Not identified so far. Expressed in ovary, testis. |
MMP24 | MT5-MMP | 73 kD | Activates Progelatinase A, degrades Proteoglycans |
MMP25 | MT6-MMP | 63 kD | Activates Progelatinase A |
MMP26 | Matrilysin 2 | 30 kD | Activates Progelatinase B, degrades other ECM components |
MMP28 | Epilysin | 59 kD | Casein |
* molecular weight of the unprocessed precursor | |||
Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts. |
Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections |
Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections |
Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections |
Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections |
Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections |
Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014) |
Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020). |
Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045). |
Available antibody panel to Matrix Metalloproteinases
Anti- Matrix Metalloproteinase-1 (MMP-1, Collagenase I), Matrix Metalloproteinase-2 (MMP-2, Gelatinase A), Matrix Metalloproteinase-3 (MMP-3, Stromelysin-1), Matrix Metalloproteinase-7 (MMP-7, Matrilysin) , Matrix Metalloproteinase-8 (MMP-8, Collagenase II), Matrix Metalloproteinase-9 (MMP-9, Gelatinase B), Matrix Metalloproteinase-10 (MMP-10, Stromelysin-2), Matrix Metalloproteinase-11 (MMP-11, Stromelysin-3), Matrix Metalloproteinase-12 (MMP-12, Metalloelastase), Matrix Metalloproteinase-13 (MMP-13, Collagenase III), Matrix Metalloproteinase-14 (MMP-14, MT1-MMP), Matrix Metalloproteinase-15 (MMP-15, MT2-MMP), Matrix Metalloproteinase-16 (MMP-16b, MT3-MMP), Matrix Metalloproteinase-17 (MMP-17, MT4-MMP), Matrix Metalloproteinase-19 (MMP-19, RASI), Matrix Metalloproteinase-20 (MMP-20, Enamelysin), Matrix Metalloproteinase-23 (MMP-23, Femalysin), Matrix Metalloproteinase-23B (MMP-23B), Matrix Metalloproteinase-24 (MMP-24, MT5-MMP), Matrix Metalloproteinase-26 (MMP-26, Matrilysin-2), Matrix Metalloproteinase-28 (MMP-28, Epilysin)
Antibodies
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| ARP42040_T100 |
MMP-1 antibody | ||||||
 |
Rabbit | Aff - Purified | Bov, Hu, Por, Rb | WB |
0.1 mg /
€290.00
|
||
| AVIVA Systems Biology | |||||||
| PY-10174 |
MMP-1 antibody | ||||||
 |
Chicken | Aff - Purified | Chk, Hu | WB |
0.1 mg /
€320.00
|
||
| Genesis Biotech Inc. | |||||||
| 29010 |
MMP-1 (Hinge Region) antibody | ||||||
|
Rabbit | Aff - Purified | Hu | C, E, IP, WB |
0.15 mg /
€420.00
|
||
| QED Bioscience Inc. | |||||||
| 10373-2-AP |
MMP-2 antibody | ||||||
 |
Rabbit | Aff - Purified | Hu | E, WB |
0.15 ml /
€255.00
|
||
| IgG | ProteinTech Group, Inc. | ||||||
 +2 additional images | |||||||
| AF6810 |
MMP-2 antibody | ||||||
|
Mouse | 42-5D11 | Purified | Hu | C, P, WB |
0.1 mg /
€360.00
|
|
| IgG1 | Acris Antibodies GmbH | ||||||
| AM00257AF-N |
MMP-2 antibody | ||||||
 |
Mouse | CA-4001 | Azide Free | Hu, Ms, Rt | FN, P, WB |
0.1 mg /
€500.00
|
|
| IgG1 | Acris Antibodies GmbH | ||||||
| AM00257PU-N |
MMP-2 antibody | ||||||
 |
Mouse | CA-4001 | Purified | Hu, Ms, Rt | IP, P, WB |
0.1 mg /
€450.00
|
|
| IgG1 | Acris Antibodies GmbH | ||||||
| AM05377PU-N |
MMP-2 antibody | ||||||
 |
Mouse | 4D3 | Purified | Hu | C, E, WB |
0.2 mg /
€300.00
|
|
| IgG1 | Acris Antibodies GmbH | ||||||
| AM08198HR-N |
MMP-2 antibody | ||||||
|
Mouse | SB13a | HRP | Hu | C, E, P |
1 ml /
€450.00
|
|
| IgG1 | Acris Antibodies GmbH | ||||||
| AM08198PU-N |
MMP-2 antibody | ||||||
|
Mouse | SB13a | Purified | Hu | C, E, P |
0.1 mg /
€370.00
|
|
| IgG1 | Acris Antibodies GmbH | ||||||
| AP00546PU-N |
MMP-2 antibody | ||||||
|
Rabbit | Purified | Bov, Hu, Ms, Rt | WB |
0.5 mg /
€450.00
|
||
| Acris Antibodies GmbH | |||||||
| AP06231PU-N |
MMP-2 antibody | ||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€320.00
|
||
| Acris Antibodies GmbH | |||||||
| AP07948PU-N |
MMP-2 antibody | ||||||
 |
Rabbit | Purified | Hu | E, P |
50 µg /
€380.00
|
||
| Acris Antibodies GmbH | |||||||
| AP13183PU-N |
MMP-2 (N-term) antibody | ||||||
 |
Rabbit | Purified | Hu | E, P, WB |
0.1 mg /
€330.00
|
||
| Ig | Acris Antibodies GmbH | ||||||
| +1 additional image | |||||||
| AP15657PU-N |
MMP-2 antibody | ||||||
 |
Rabbit | Purified | Hu | P |
1 ml /
€400.00
|
||
| Acris Antibodies GmbH | |||||||
| AP15657PU-S |
MMP-2 antibody | ||||||
|
Rabbit | Purified | Hu | P |
Regular Price: 0.1 ml / €170.00 Special Price: 0.1 ml / €105.00 |
||
| Acris Antibodies GmbH | |||||||
| BP613 |
MMP-2 antibody | ||||||
|
Sheep | Ig Fraction | Hu | P, WB |
1 ml /
€380.00
|
||
| Acris Antibodies GmbH | |||||||
| DM312 |
MMP-2 antibody | ||||||
 |
Mouse | A-Gel VC2 | Supernatant | Hu | C, P |
1 ml /
€510.00
|
|
| IgG1 | Acris Antibodies GmbH | ||||||
| DM312-05 |
MMP-2 antibody | ||||||
|
Mouse | A-Gel VC2 | Supernatant | Hu | C, P |
0.5 ml /
€350.00
|
|
| IgG1 | Acris Antibodies GmbH | ||||||
| NB100-65822 |
MMP-2 antibody | ||||||
|
Sheep | Aff - Purified | Hu | P, WB |
0.5 ml /
€470.00
|
||
| IgG | Novus Biologicals Inc. | ||||||
