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Antibody Panel to Actin - Product Review 08

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Introduction

Actin is an abundant cytoskeletal protein found in all cells.

The protein's 42kD peptide chain assumes two physical forms: globular actin (G-actin), which may serve as a cytoplasmic storage pool, and fibrous actin (F-actin) which, in conjunction with myosin, generates muscle contraction. In non-muscle cells, actin appears to be involved in a variety of functions such as cell motility, exocytosis and phagocytosis.

Antibody Tools for Detection of Actin

Acris Antibodies offers a broad panel of antibodies to actin which can be used in various detection methods like immunohistochemistry, ELISA or Western blotting. Table 1 shows a list of all available antibodies to actin.

Picture Gallery

Staining of human muscle using DM002 anti alpha actin monoclonal antibody Staining of human leiomyoma using DM001 anti alpha smooth muscle monoclonal antibody

Fig.1: Staining of human muscle using DM002 anti alpha/gamma actin monoclonal antibody.

Fig.2: Staining of human leiomyoma using DM001 anti alpha smooth muscle monoclonal antibody.

Available Antibody Panel to Actin

Anti- Actin, F-Actin, G-Actin, alpha Actin, beta Actin antibody

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47 products - FILTER YOUR RESULTS!

Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
14395-1-AP

ACTA2 / aortic smooth muscle Actin antibody

K562 cell were subjected to SDS PAGE followed by western blot with 14395-1-AP(ACTA2 Antibody) at dilution of 1:800 Rabbit Aff - Purified Hu, Ms E, P, WB
0.15 ml / €255.00
  IgG   ProteinTech Group, Inc.
+2 additional images
 
AP08847PU-N

ACTA2 / aortic smooth muscle Actin (N-term) antibody

Colon, vessel: Formalin-Fixed Paraffin-Embedded (FFPE) using ACTA2 antibody Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rb, Rt P
50 µl / €380.00
  IgG   Acris Antibodies GmbH
 
AP16262PU-N

ACTA2 / aortic smooth muscle Actin (N-term) antibody

AP16262PU-N staining (0.3 µg/ml) of Human Duodenum lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €270.00
    Acris Antibodies GmbH
 
NB300-978

ACTA2 / aortic smooth muscle Actin antibody

Western Blot: Smooth muscle alpha-actin Antibody [NB300-97]- staining (0.1ug/ml) of Human Duodenum lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Goat Aff - Purified Hu, Ms, Por, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  IgG   Novus Biologicals Inc.
 
NB600-531

ACTA2 / aortic smooth muscle Actin antibody

Formalin-fixed, paraffin-embedded Human Leiomyoma stained with NB 600-531 Rabbit Aff - Purified Hu P
0.5 ml / €310.00
    Novus Biologicals Inc.
 
NB600-1434

ACTA2 / aortic smooth muscle Actin antibody

Formalin-fixed, paraffin-embedded human leiomyoma stained with Actin using peroxidase-conjugate and AEC. Note cytoplasmic staining of tumor cells. Rabbit Aff - Purified Hu, Mky, Ms, Rb, Rt P
7 ml / €370.00
  IgG   Novus Biologicals Inc.
 
NBP1-18879

ACTA2 / aortic smooth muscle Actin antibody

Western Blot and Immunoprecipitation: Alpha Smooth Muscle Actin Antibody [NBP1-18879] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €370.00
  IgG   Novus Biologicals Inc.
 
NBP1-19535

ACTA2 / aortic smooth muscle Actin antibody

Immunohistochemistry-Paraffin: Actin Alpha 2 Smooth Muscle Antibody [NBP1-19535] - Paraffin-embedded human muscle tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
    Novus Biologicals Inc.
+1 additional image
 
GTX100034

ACTA2 / aortic smooth muscle Actin antibody

ACTA2 / aortic smooth muscle Actin Rabbit Aff - Purified Hu ICC/IF, WB
0.1 ml / €300.00
    GeneTex Inc.
 
GTX100095

ACTA2 / aortic smooth muscle Actin antibody

ACTA2 / aortic smooth muscle Actin Rabbit Aff - Purified Hu C, WB
0.1 ml / €300.00
    GeneTex Inc.
 
GTX112862

ACTA2 / aortic smooth muscle Actin antibody

ACTA2 / aortic smooth muscle Actin Rabbit Aff - Purified Hu WB
0.1 ml / €300.00
    GeneTex Inc.
 
NB100-906

Actin (2 vial set) antibody

Western Blot: Actin antibody [NB600-533] - Detection of actin in 3T3 (20ug) lysates using NB 600-532. ECL detection 30 seconds. Lane 1 - 1:15,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - 1:15,000 dilution Rabbit Aff - Purified Hu, Ms IP, WB
2 vials / €460.00
    Novus Biologicals Inc.
+1 additional image
 
10230-1-AP

Actin beta / ACTB antibody

Actin beta / ACTB Rabbit Aff - Purified Hu, Ms E
0.15 ml / €255.00
  IgG   ProteinTech Group, Inc.
 
AP00310PU-N

Actin beta / ACTB (Loading Control) antibody

Western blot analysis of β-Actin in Jurkat cell lysate (left lane) and mouse intestine tissue lysate (right lane). Rabbit Aff - Purified Hu, Ms, Rb WB
0.1 mg / €320.00
    Acris Antibodies GmbH
 
AP07457PU-N

Actin beta / ACTB antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €380.00
  IgG   Acris Antibodies GmbH
 
AP07540PU-N

Actin beta / ACTB antibody

Skeletal muscle, vessel: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €380.00
    Acris Antibodies GmbH
 
AP08869PU-N

Actin beta / ACTB antibody

Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €380.00
  IgG   Acris Antibodies GmbH
 
AP08996PU-N

Actin beta / ACTB (N-term) antibody

Leiomyoma stained with Anti-beta Actin Antibody Rabbit Aff - Purified Bov, Can, Chk, Fe, GP, Hu, Ms, Por, Rb, Rt, Sh P, WB
50 µg / €380.00
    Acris Antibodies GmbH
+1 additional image
 
AP09293PU-N

Actin beta / ACTB (359-368) (Loading Control) antibody

Western blot using Affinity Purified anti-beta Actin antibody shows detection of a predominant band at ~42 kDa corresponding to beta Actin (arrowhead) in various whole cell lysates. Lysates are as follows: human embryonic kidney 293 (lane 2), human lung carcinoma A549 (lane 3) and mouse brain (lane 4) all using the 800 nm channel (green). ~ 35 µg of each lysate was separated on a 4-20% Tris-glycine gel by SDSPAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Incubation was overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Molecular weight markers are shown in lane 1 using the 700 nm channel (red). IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit Aff - Purified Hu E, P, WB
0.2 mg / €300.00
  IgG   Acris Antibodies GmbH
+1 additional image
 
NB100-78420

Actin beta / ACTB antibody

western blot: beta actin Antibody [NB100-78420] -Hela cell extract Poly6221. Proteins were visualized using a HRP donkey anti-rabbit antibody and a chemiluminescence detection system. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.2 ml / €390.00
  IgG   Novus Biologicals Inc.
+2 additional images
 

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