Antibody Panel to Actin - Product Review 08

Introduction

Actin is an abundant cytoskeletal protein found in all cells.

The protein's 42kD peptide chain assumes two physical forms: globular actin (G-actin), which may serve as a cytoplasmic storage pool, and fibrous actin (F-actin) which, in conjunction with myosin, generates muscle contraction. In non-muscle cells, actin appears to be involved in a variety of functions such as cell motility, exocytosis and phagocytosis.

Antibody Tools for Detection of Actin

Acris Antibodies offers a broad panel of antibodies to actin which can be used in various detection methods like immunohistochemistry, ELISA or Western blotting. Table 1 shows a list of all available antibodies to actin.

Picture Gallery

Staining of human muscle using DM002 anti alpha actin monoclonal antibody Staining of human leiomyoma using DM001 anti alpha smooth muscle monoclonal antibody

Fig.1: Staining of human muscle using DM002 anti alpha/gamma actin monoclonal antibody.

Fig.2: Staining of human leiomyoma using DM001 anti alpha smooth muscle monoclonal antibody.

Available Antibody Panel to Actin

Anti- Actin, F-Actin, G-Actin, alpha Actin, beta Actin antibody

9 Item(s)

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Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
AP00310PU-N

Actin beta / ACTB (Loading Control) antibody

Western blot analysis of β-Actin in Jurkat cell lysate (left lane) and mouse intestine tissue lysate (right lane). Rabbit Aff - Purified Hu, Ms, Rb WB
0.1 mg / €320.00
    Acris Antibodies GmbH
 
AP07129PU-N

Actin beta / ACTB antibody

Prostate: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €380.00
    Acris Antibodies GmbH
 
AP07457PU-N

Actin beta / ACTB antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €380.00
  IgG   Acris Antibodies GmbH
 
AP07540PU-N

Actin beta / ACTB antibody

Skeletal muscle, vessel: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €380.00
    Acris Antibodies GmbH
 
AP08869PU-N

Actin beta / ACTB antibody

Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €380.00
  IgG   Acris Antibodies GmbH
 
AP08996PU-N

Actin beta / ACTB (N-term) antibody

Leiomyoma stained with Anti-beta Actin Antibody Rabbit Aff - Purified Bov, Can, Chk, Fe, GP, Hu, Ms, Por, Rb, Rt, Sh P, WB
50 µg / €380.00
    Acris Antibodies GmbH
 
AP09293PU-N

Actin beta / ACTB (359-368) (Loading Control) antibody

Western blot using Affinity Purified anti-beta Actin antibody shows detection of a predominant band at ~42 kDa corresponding to beta Actin (arrowhead) in various whole cell lysates. Lysates are as follows: human embryonic kidney 293 (lane 2), human lung carcinoma A549 (lane 3) and mouse brain (lane 4) all using the 800 nm channel (green). ~ 35 µg of each lysate was separated on a 4-20% Tris-glycine gel by SDSPAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Incubation was overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Molecular weight markers are shown in lane 1 using the 700 nm channel (red). IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit Aff - Purified Hu E, P, WB
0.2 mg / €300.00
  IgG   Acris Antibodies GmbH
 
AP15333PU-N

Actin beta / ACTB (Loading Control) antibody

Leiomyoma stained with Anti-beta Actin Rabbit Purified Bov, Can, Chk, Fe, GP, Hu, Ms, Por, Rb, Rt, Sh P, WB
1 ml / €370.00
    Acris Antibodies GmbH
 
AP15333PU-S

Actin beta / ACTB (Loading Control) antibody

Leiomyoma stained with Anti-beta Actin Rabbit Purified Bov, Can, Chk, Fe, GP, Hu, Ms, Por, Rb, Rt, Sh P, WB
0.1 ml / €170.00
    Acris Antibodies GmbH
 

9 Item(s)

per page