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Home » Reviews » Antibody Panel to Cathepsin Protease Family - Product Review 26

Antibody Panel to Cathepsin Protease Family - Product Review 26

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Introduction: The Cathepsins are a group of lysosomal thiol proteinases or endopeptidases found in extracts of various tissues.

Cathepsins, with the exception of Cathepsin S, are active under acidic, but unstable in neutral or alkaline conditions. Cathesin S is active at a neutral pH. The Cathepsins are synthesised by the conversion of a precursor into the mature glycoprotein. Cathepsin activity is regulated by the binding of endogenous protein inhibitors, called cystatins of which Stefin A and B are family members. They have been shown to be important in main physiological and pathological processes and to be of prognostic significance in a number of cancers.

In breast, lung, brain, head and neck cancer a significant prognostic value has been found measuring Cathepsin B activity either by protein assay or immunohistochemical determination. Serum levels of Cathepsin H appeared as prognostic markers for melanoma patients and predicted the effectiveness of the chemoimmunotherapy of these patients. In breast cancer over-expression of Cathepsin L is defined of one of the strongest prognostic factors for a subgroup of patients with node negative breast tumors.

Table 1: Cathepsin Protease Overview

Cathepsin

Molecular Weight* kDa

Cellular Localisation

Tissue Specificity

Function

Cathepsin B

37.8

Lysosomes

no

Thiol Protease

Cathepsin C

51.8

Lysosomes

no

Thiol Protease

Cathepsin D

44.5

Lysosomes

no

Acid Protease

Cathepsin E

43.3

Endosomes

no

Acid Protease

Cathepsin F

53.3

Lysosomes

heart, muscle, brain testis, ovary

Thiol Protease

Cathepsin G

29.0

Membranes

no

Vimentin specific protease

Cathepsin H

37.4

Lysosomes

no

Aminopeptidase

Cathepsin K

37.0

Lysosomes

osteoclasts

Endopeptidase

Cathepsin L

37.5

Lysosomes

no

Thiol Protease

Cathepsin L2/U/V

37.3

Lysosomes

thymus, testis, cornea

Cysteine Protease

Cathepsin O

36.0

Lysosomes

no

Endopeptidase

Cathepsin S

37.5

Lysosomes

no

Thiol Protease

Cathepsin W

42.1

Lysosomes

NK cells,
cytotoxic T-Cells

?

Cathepsin Z

33.9

Lysosomes

no

Carboxydipeptidase

* molecular weight of the unprocessed precursor

Antibody Tools for Detection of Cathepsin Proteases

Antibody products for the detection of most of the above listed Cathepsins are available at Acris Antibodies. These antibodies are suitable for various immunological procedures like immunohistochemistry, ELISA, immunoprecipitation or Western blotting.



Staining of breast carcinoma paraffin sections using anti Cathepsin D monoclonal antibody code BM104

Staining of breast carcinoma paraffin sections using anti Cathepsin D monoclonal antibody code BM104

Staining of human lymph node tissue using anti Cathepsin S monoclonal antibody code SM1669P Intense staining of antigen presenting cells in trabecular and subcapsular sinuses can be seen.

Staining of human lymph node tissue using anti Cathepsin S monoclonal antibody code SM1669P Intense staining of antigen presenting cells in trabecular and subcapsular sinuses can be seen.

Staining of mouse ventral spinal cord using anti Cathepsin D polyclonal antibody code GT15042

Staining of mouse ventral spinal cord using anti Cathepsin D polyclonal antibody code GT15042

  


Literature:

Ref. 1
Obermajer N, Doljak B, Kos J. Cysteine cathepsins: regulators of antitumour immune response. Expert Opin Biol Ther. 2006 Dec;6(12):1295-309.
Ref. 2
Nomura T, Katunuma N. Involvement of cathepsins in the invasion, metastasis and proliferation of cancer cells. J Med Invest. 2005 Feb;52(1-2):1-9.


Available Antibodies to Cathepsin Proteases
Cathepsin B, Cathepsin D, Cathepsin F, Cathepsin G, Cathepsin H, Cathepsin K, Cathepsin L, Cathepsin L2 (U, V), Cathepsin W, Cathepsin Z ? antibody and Protective Protein / Cathepsin A (PPCA) antibody



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Antibodies

Catalog No. Host Clone/Iso. Pres.   React. Applications  
12216-1-AP

Cathepsin B antibody

Human brain were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB Antibody) at dilution of 1:1000 Rabbit Aff - Purified Hu, Ms E, WB
0.15 ml / €255.00
  IgG   ProteinTech Group, Inc.
 
AP00064CP-N

Cathepsin B Control Peptide

Cathepsin B Purified
50 µg / €180.00
    Acris Antibodies GmbH
 
AP00064PU-N

Cathepsin B antibody

Western blot analysis of Cathepsin B with Jurkat cell (Lane 1) and mouse small intestine tissue (Lane 2) lysate. Rabbit Purified Hu, Ms, Rt P, WB
0.1 mg / €320.00
    Acris Antibodies GmbH
 
AP08517PU-N

Cathepsin B antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Ms, Rt P, WB
50 µg / €410.00
  IgG   Acris Antibodies GmbH
 
SM2106P

Cathepsin B antibody

Cathepsin B Mouse CB131 Supernatant Hu P
1 ml / €490.00
  IgG2b   Acris Antibodies GmbH
 
SP1127P

Cathepsin B antibody

Cathepsin B Sheep Purified Hu ID, WB
1 ml / €390.00
    Acris Antibodies GmbH
 
NB600-1111

Cathepsin B antibody

Cathepsin B Mouse CB131 Hu P
0.5 ml / €390.00
  IgG2b   Novus Biologicals Inc.
 
NBP1-19797

Cathepsin B antibody

Immunohistochemistry: Cathepsin B Antibody [NBP1-19797] - Cathepsin B Antibody [NBP1-19797] - paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
    Novus Biologicals Inc.
+1 additional image
 
NBP1-21424

Cathepsin B antibody

ELISA: CATHEPSIN B Antibody [NBP1-21424] Sheep HRP Hu E
1 ml / €340.00
  IgG   Novus Biologicals Inc.
 
NBP1-25931

Cathepsin B antibody

Cathepsin B Sheep FITC Hu E
0.5 ml / €370.00
    Novus Biologicals Inc.
 
NBP1-25932

Cathepsin B antibody

ELISA: Cathepsin B Antibody [NBP1-25932] - Cathepsin B detected with Sheep anti Cathepsin B Sheep Purified Hu E
1 ml / €330.00
    Novus Biologicals Inc.
 
GT15046-100

Cathepsin B antibody

Cathepsin B Goat Aff - Purified Hu WB
0.1 mg / €430.00
    Neuromics Antibodies
 
GT15047-100

Cathepsin B antibody

Cells were scratch-wounded and stained against cathepsin B and annexin-II heterotetramer subunit p11 after 1 (a), 8 (b) and 24 (c) hours. Fixation was performed with both -20oC cold methanol and acetone for 8 minutes each. Cathepsin B is shown in red, p11 is shown in green. Goat Aff - Purified Ms C, E, WB
0.1 mg / €430.00
    Neuromics Antibodies
 
AM09003PU-N

Cathepsin D antibody

Western blot analysis: Cell lysates of HepG2 (lane 1), Hep3B (lane 2), MDA-MB-231 (lane 3) and MCF7 (lane 4) -each 20 ug- were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system. Mouse 4G2 Purified Hu E, P, WB
0.1 ml / €310.00
  IgG2b   Acris Antibodies GmbH
+6 additional images
 
AM09003PU-S

Cathepsin D antibody

Western blot analysis: Cell lysates of HepG2 (lane 1), Hep3B (lane 2), MDA-MB-231 (lane 3) and MCF7 (lane 4) -each 20 ug- were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system. Mouse 4G2 Purified Hu E, P, WB
50 µl / €230.00
  IgG2b   Acris Antibodies GmbH
+6 additional images
 
AP15400PU-N

Cathepsin D antibody

Human breast carcinoma stained with anti-cathepsin D antibody Rabbit Purified Hu P
1 ml / €370.00
    Acris Antibodies GmbH
 
AP15400PU-S

Cathepsin D antibody

Human breast carcinoma stained with anti-cathepsin D antibody Rabbit Purified Hu P
0.1 ml / €170.00
    Acris Antibodies GmbH
 
BM104

Cathepsin D antibody

Cathepsin D Mouse C5 Supernatant Hu C, P
0.2 ml / €300.00
  IgG2b   Acris Antibodies GmbH
 
BP2197

Cathepsin D antibody

Cathepsin D Rabbit Purified Hu E, WB
1 ml / €350.00
    Acris Antibodies GmbH
 
DP004

Cathepsin D antibody

Cathepsin D Rabbit Purified Hu C, P
1 ml / €430.00
    Acris Antibodies GmbH
 

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