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Secondary Antibodies

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Secondary Antibodies

Catalog No. Host Pres.  
R1317AP

Goat IgG (H+L chain)

Western Blot of Rabbit anti-Goat Antibody Alkaline Phosphatase Conjugated: Lane 1: Goat IgG, Load: 100 ng per lane Secondary antibody Alkaline Phosphatase Conjugated Rabbit Anti-Goat Antibody at 1/1000 for 60 min at RT Block: MB-070 30 min RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Rabbit AP  
1 mg / €190.00
  Acris Antibodies GmbH
 
R1317F

Goat IgG (H+L chain)

Western Blot of Rabbit anti-Goat IgG (H&L) Antibody Fluorescein Conjugated: Lane 1: Goat IgG, Load: 50 ng per lane Secondary antibody: Goat IgG (H&L) Antibody Fluorescein Conjugated at 1/1,000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Rabbit FITC  
2 mg / €180.00
  Acris Antibodies GmbH
 
R1317HRP

Goat IgG (H+L chain)

Western Blot of Rabbit anti-GOAT IgG (H&L) Antibody Peroxidase Conjugated:  Lane 1: Goat IgG, Load: 100 ng per lane Secondary antibody GOAT IgG (H&L) Antibody Peroxidase Conjugated at 1/1,000 for 60 min at RT Block: MB-070 for 30 min at RT Rabbit HRP  
2 mg / €190.00
  Acris Antibodies GmbH
 
R1318HRP

Goat IgG (H+L chain), adsorbed

Western Blot of HRP conjugated anti-Goat IgG antibody R1318HRP showing detection of 50ng of Goat IgG (Lane 1) but not Human IgG (Lane 2). Samples were separated by 4-20% SDS-PAGE under reducing conditions and transferred to nitrocellulose membrane. The blot was blocked overnight at 4°C in 5% BSA in TBS. A 1/5,000 dilution of antibody in Blocking Buffer for Fluorescent Western Blotting was used to probe the membrane at RT for 1 h. The image was developed using Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate (p/n Femtomax-020) for one minute. Rabbit HRP  
1 mg / €210.00
  Acris Antibodies GmbH
 
SP1025B

Hamster IgG (adsorbed)

Detection of Hamster anti Human EMR1 (MCA2674) in a Flow Cytometric analysis of Human eosinophils using Goat anti Hamster IgG -Biotin (Cat.-No SP1025B) visualised by Streptavidin:RPE (STAR4A) Goat Biotin  
0.8 mg / €660.00
  Acris Antibodies GmbH
+1 additional image
 
R1253HRP

Mouse IgG (H+L chain)

Western Blot of Mouse IgG Antibody Peroxidase Conjugated detecting mouse IgG. Mouse IgG (1.0 mg) was separated on a 4-20% gradient gel and then transferred to Whatman Protran BA 85 nitrocellulose (0.45 µm pore size) and blocked with 5% BLOTTO (p/n B501-0500) o/n at 4°C. The membrane was then probed with a 1/2,500 dilution of HRP Rabbit anti-Mouse IgG [H&L] R1253HRP for 45 min at RT. FemtoMax Super Sensitive Chemiluminescent HRP Substrate Was added and the image was captured using a BioSpectrum Imaging System with a BioChemi 500 -28C cooled 4.0 Megapixel CCD Camera from UVP (www.uvp.com, Upland, CA). Binning: 5X5. Exposure time: 2 min. Aperture open. No emission or excitation filters used. Rabbit HRP  
2 mg / €190.00
  Acris Antibodies GmbH
 
SP1041F

Mouse IgG (H+L chain), adsorbed

Detection of Mouse anti Bovine CD80 Antibody in a flow cytometric analysis of bovine peripheral blood monocytes using Goat anti Mouse IgG Antibody -FITC Cat.-No SP1041F Goat FITC  
0.5 mg / €310.00
  Acris Antibodies GmbH
+3 additional images
 
GT26000-1000

Mouse IgG

HeLa cells were stained with alpha Tubulin mouse mAb (Clone 5-B-1-2) and Chromeo 488 Goat anti-mouse IgG. The nuclei have been counterstained with DAPI.  Goat Chromeo™ sity 488  
1 mg / €280.00
  Neuromics Antibodies
+1 additional image
 
GT26003-1000

Mouse IgG

The Chromeo 546 Goat anti-Mouse IgG secondary antibody was used to stain EGFR in an urothel cell line. This data was generously provided by Dr. Brockhoff, Institut for Pathology, University Regensburg, Germany. Goat Chromeo™ sity 546  
1 mg / €280.00
  Neuromics Antibodies
 
GT26005-1000

Mouse IgG

Spectrum of CHROMEO™sity  642 conjugated Goat anti-Mouse IgG. Goat Chromeo™ sity 642  
1 mg / €280.00
  Neuromics Antibodies
 
R1364HRP

Rabbit IgG (H+L chain)

Western Blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody: Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH/3T3 Whole Cell Lysate. Load: 10 µg per lane. Primary antibody: Beta Actin Antibody at 1/1000 for overnight at 4°C. Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT . Predicted/Observed size: 42 kDa. Goat HRP  
2 mg / €180.00
  Acris Antibodies GmbH
 
R1454HRP

Rabbit IgG (H+L chain, adsorbed)

Western Blot showing detection of 0.1 µg of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBPp27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1/1000 at RT for 30 min. HRP-conjugated Goat-Anti-Rabbit R1454HRP secondary antibody was used at 1/40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad). Goat HRP  
1 mg / €250.00
  Acris Antibodies GmbH
+1 additional image
 
SP2041HRP

Rabbit IgG (H+L chain)

Detection of Rabbit anti Human XRCC4 Antibody Cat.-No SP1141 in a Western blot analysis of a HeLa cell nuclear extract using Sheep anti Rabbit IgG Antibody -HRP Cat.-No SP2041HRP Sheep HRP  
1 mg / €510.00
  Acris Antibodies GmbH
+2 additional images
 
GT26001-1000

Rabbit IgG

Spectrum of CHROMEO™sity  488 conjugated Goat anti-Rabbit IgG. Goat Chromeo™ sity 488  
1 mg / €280.00
  Neuromics Antibodies
 
GT26002-1000

Rabbit IgG

HeLa cells were stained with rabbit alpha Tubulin polyclonal antibody and Chromeo 494 Goat anti-rabbit IgG. Goat Chromeo™ sity 494  
1 mg / €280.00
  Neuromics Antibodies
+1 additional image
 
GT26004-1000

Rabbit IgG

Spectrum of CHROMEO™sity  546 conjugated Goat anti-Rabbit IgG. Goat Chromeo™ sity 546  
1 mg / €280.00
  Neuromics Antibodies
 
GT26006-1000

Rabbit IgG

CD39 distribution in rat pancreatic ducts. J Physiol (2003), 551.3, pp. 881-892 © Copyright 2003 The Physiological Society. DOI: 10.1113/jphysiol.2003.049411 Goat Cy5  
1 mg / €280.00
  Neuromics Antibodies
 

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Product Type
  1. Secondary Antibodies (17)
Clonality
  1. Polyclonal (17)
Presentation
  1. HRP (6)
  2. Chromeo™ sity 488 (2)
  3. Chromeo™ sity 546 (2)
  4. FITC (2)
  5. AP (1)
  6. Biotin (1)
  7. Chromeo™ sity 494 (1)
  8. Chromeo™ sity 642 (1)
  9. Cy5 (1)
Host
  1. Goat (11)
  2. Rabbit (5)
  3. Sheep (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (8)
  2. Flow cytometry (F) (2)
  3. Paraffin Sections (P) (1)
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  1. Acris Antibodies GmbH (10)
  2. Neuromics Antibodies (7)
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