Product Description for Chicken IgG / Chicken IgY
Properties: (Fc specific)
Properties for Chicken IgG / Chicken IgY
|Product Category||Secondary Antibodies|
|Synonyms||Chicken Immunoglobulin G, Chicken Immunoglobulin Y|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Application||Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
|Concentration||1.5 mg/ml (by UV absorbance at 280 nm)|
|General Readings||Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)|
|Storage||Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.