R1254F Goat IgG (H+L chain), adsorbed

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1 mg / €220.00
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R1254F

Product Description for Goat IgG

Properties: (H+L chain), adsorbed
Presentation: FITC

Properties for Goat IgG

Product Category Secondary Antibodies
Quantity 1 mg
Synonyms Goat Immunoglobulin G
Presentation FITC
Clonality Polyclonal
Host Donkey
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Immunogen:
Goat IgG whole molecule.
Application Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings The and Feltkamp, Immunology 18; 865, 1970. (Conjugation)
Storage Store vial at 2-8°C prior to restoration.
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for long term storage.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Fluorescein isothiocyanate , Molecular Weight 390 daltons)
FITC
Absorption emission:
495 nm / 528 nm
Molar radio:
3.9 moles FITC per mole of Donkey IgG.
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Goat IgG and Goat Serum.
No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.
Targets