R1318HRP Goat IgG (H+L chain), adsorbed

Search for all "Goat IgG"

1 mg / €210.00
Delivery Time: 5-7 working days

Product Description for Goat IgG

Properties: (H+L chain), adsorbed
Presentation: HRP

Properties for Goat IgG

Product Category Secondary Antibodies
Quantity 1 mg
Synonyms Goat Immunoglobulin G
Presentation HRP
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Immunogen:
Goat IgG whole molecule
Application Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  • Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)
Storage Store vial at 2-8°C prior to restoration.
Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Buffer System:
0.02 MPotassium Phosphate, 0.15M Sodium Chloride, pH 7.2, with 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
Purity:
and
State:
Lyophilized purified Ig fraction
Reconstitution:
Restore with 1.0 ml deionized water or equivalent.
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum.
No reaction was observed against Human Serum Proteins.
Targets