R1323HRP Guinea pig IgG (Fc specific) antibody

Search for all "Guinea pig IgG"

2 mg / €230.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

R1323HRP

Product Description for Guinea pig IgG

Properties: (Fc specific)
Presentation: HRP

Properties for Guinea pig IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Guinea pig Immunoglobulin G
Presentation HRP
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Guinea Pig IgG F(c) fragment.
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 is suggested for this product.
Caution Do Not Use Sodium Azide as preservative.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml Bovine Serum Albumin (BSA) (IgG and Protease free) as stabilizer.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum.
No reaction was observed against Guinea Pig IgG F(ab')2 fragment.
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