R1328HRP Hamster IgG (F(ab)2 specific)

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1 mg / €220.00
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R1328HRP

Product Description for Hamster IgG

Properties: (F(ab)2 specific)
Presentation: HRP

Properties for Hamster IgG

Product Category Secondary Antibodies
Quantity 1 mg
Synonyms Hamster Immunoglobulin G
Presentation HRP
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Immunogen:
Hamster IgG F(ab')2 fragment
Application Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Hamster IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:30,000 of the reconstitution concentration is suggested for this product.
Caution Do Not Use Sodium Azide as preservative.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Hamster IgG, Hamster IgG F(ab')2 and Hamster Serum.
No reaction was observed against Hamster IgG F(c).
Targets