R1253B Mouse IgG (H+L chain)

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2 mg / €190.00
Delivery Time: 24 hours

R1253B

Product Description for Mouse IgG

Properties: (H+L chain)
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Mouse IgG

Product Category Secondary Antibodies
Quantity 2 mg
Synonyms Mouse Immunoglobulin G
Presentation Biotin
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG whole molecule.
Application Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  • Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
Storage Store vial at 2-8°C prior to restoration.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Rabbit IgG molecule
Purification:
Immunoaffinity Chromatography
Buffer System:
0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
10 mg/ml BSA (IgG and Protease free)
Purity:
and
State:
Lyohilized purified Ig fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Targets